CAJ | 학술논문

目的：：观察直接心肌注入 microRNA-21基因腺病毒对大鼠心肌梗死面积的影响。方法：40只健康SD 大鼠,随机分为五组：假手术组、模型组、生理盐水组、腺病毒载体组、microRNA-21腺病毒组,每组8只。假手术组只开胸、穿线,不结扎,也不注射任何物质。余4组通过结扎冠状动脉左前降支复制心肌梗死模型,其中模型组成模后不做其它处理,另3组成模后分别注入不同目标物质。饲养3天后一并处死,摘取心脏,采用实时荧光定量 PCR 检测左心室心肌 microRNA-21表达量,采用 TTC 染色法测量心肌梗死面积。统计学分析各组上述指标间的差异。结果：microRNA-21腺病毒组 microRNA-21表达量(0.72±0.01)较其它四组明显增加(P <0.01),其它四组间差异无统计学意义(P >0.05)；假手术组未见心肌梗死,microRNA-21腺病毒组心肌梗死面积(31.27±1.60%)较其它三组明显减小(P <0.05),其它三组心肌梗死面积差异均无统计学意义(P >0.05)。结论：直接心肌注射 microRNA-21基因腺病毒可实现心肌 microRNA-21基因的过表达,进而减小心肌梗死面积。
목적：：관찰직접심기주입 microRNA-21기인선병독대대서심기경사면적적영향。방법：40지건강SD 대서,수궤분위오조：가수술조、모형조、생리염수조、선병독재체조、microRNA-21선병독조,매조8지。가수술조지개흉、천선,불결찰,야불주사임하물질。여4조통과결찰관상동맥좌전강지복제심기경사모형,기중모형조성모후불주기타처리,령3조성모후분별주입불동목표물질。사양3천후일병처사,적취심장,채용실시형광정량 PCR 검측좌심실심기 microRNA-21표체량,채용 TTC 염색법측량심기경사면적。통계학분석각조상술지표간적차이。결과：microRNA-21선병독조 microRNA-21표체량(0.72±0.01)교기타사조명현증가(P <0.01),기타사조간차이무통계학의의(P >0.05)；가수술조미견심기경사,microRNA-21선병독조심기경사면적(31.27±1.60%)교기타삼조명현감소(P <0.05),기타삼조심기경사면적차이균무통계학의의(P >0.05)。결론：직접심기주사 microRNA-21기인선병독가실현심기 microRNA-21기인적과표체,진이감소심기경사면적。
Objective:To investigate the effects on myocardial infarction(MI)size through injecting gene ade-novirus carrying micro RNA-21 (miR-21)into the myocardium directly.Method:Forty male mice were divided into sham group,model group,saline group,adenovirus vector group,miR-21 adenovirus group.The sham group was only opened and threaded without ligation and infusion.The remaining four groups were subjected to MI by left an-terior descending artery (LAD)ligation,model group was left untreated,while the other three groups were infused different target materials into surrounding areas of MI after building model successfully.Then executed the mice and spayed the heart at 3th day concurrent.Expression of miR-21 in surrounding areas of left ventricular MI was detec-ted with real-time PCR and MI size was measured with TTC dyeing.Results:The expression of micrornas-21 of in-jection with microras-21 adenovirus vector group (0.72 ± 0.01 )was significantly increased than the other four groups(P <0.01).There was no obvious differences among the other four groups;sham group had no myocardial infarction,while myocardial infarction size of micrornas-21 adenovirus vector group (31.27 ± 1.60%)significantly decreased than the other three groups (P <0.05).There were no significant statistical differences among them.Con-clusion:Intramyocardial injections by directly injection with miR-21 gene adenovirus vectors can realize overexpres-sion of miR-21 gene,the gene expression of miR-21 in three days after myocardial infarction has the effect that re-duce MI size.