微循环学杂志
微循環學雜誌
미순배학잡지
Chinese Journal of Microcirculation
2015年
4期
11-14
,共4页
刘蜜%宋丹丹%李玉珍%徐菲菲%陶天琪%李冬%刘秀华%郭渝成
劉蜜%宋丹丹%李玉珍%徐菲菲%陶天琪%李鼕%劉秀華%郭渝成
류밀%송단단%리옥진%서비비%도천기%리동%류수화%곽투성
创伤后应激障碍%肠系膜%软脑膜%微循环%大鼠
創傷後應激障礙%腸繫膜%軟腦膜%微循環%大鼠
창상후응격장애%장계막%연뇌막%미순배%대서
Post-traumatic stress disorder%Mesentery%Cerebral piamater%Microcirculation%Rat
目的：：研究创伤后应激障碍(PTSD)大鼠肠系膜和软脑膜微循环的改变及其机制。方法：成年健康雄性 SD 大鼠18只,随机分为正常对照组(n=6)和 PTSD 组(n=12)。采用单程延长刺激法复制 PTSD 大鼠模型,通过旷场实验检测大鼠的行为学改变；采用活体微循环观测技术观察大鼠肠系膜及软脑膜微血管内径和血流速度的改变；采用 ELISA 检测大鼠血浆去甲肾上腺素(NA)、内皮型一氧化氮合酶(eNOS)和内皮素-1(ET-1)水平。结果：与正常对照组比较,PTSD 组大鼠在旷场中穿行格数及直立次数显著减少(P <0.01)；肠系膜及软脑膜细动脉、细静脉管径明显变细(P <0.05),血流速度趋缓；血浆 NA 和 ET-1含量显著升高(P <0.01),但 eNOS 水平无显著变化(P >0.05)。结论：PTSD 大鼠存在肠系膜和软脑膜微循环障碍。
目的：：研究創傷後應激障礙(PTSD)大鼠腸繫膜和軟腦膜微循環的改變及其機製。方法：成年健康雄性 SD 大鼠18隻,隨機分為正常對照組(n=6)和 PTSD 組(n=12)。採用單程延長刺激法複製 PTSD 大鼠模型,通過曠場實驗檢測大鼠的行為學改變；採用活體微循環觀測技術觀察大鼠腸繫膜及軟腦膜微血管內徑和血流速度的改變；採用 ELISA 檢測大鼠血漿去甲腎上腺素(NA)、內皮型一氧化氮閤酶(eNOS)和內皮素-1(ET-1)水平。結果：與正常對照組比較,PTSD 組大鼠在曠場中穿行格數及直立次數顯著減少(P <0.01)；腸繫膜及軟腦膜細動脈、細靜脈管徑明顯變細(P <0.05),血流速度趨緩；血漿 NA 和 ET-1含量顯著升高(P <0.01),但 eNOS 水平無顯著變化(P >0.05)。結論：PTSD 大鼠存在腸繫膜和軟腦膜微循環障礙。
목적：：연구창상후응격장애(PTSD)대서장계막화연뇌막미순배적개변급기궤제。방법：성년건강웅성 SD 대서18지,수궤분위정상대조조(n=6)화 PTSD 조(n=12)。채용단정연장자격법복제 PTSD 대서모형,통과광장실험검측대서적행위학개변；채용활체미순배관측기술관찰대서장계막급연뇌막미혈관내경화혈류속도적개변；채용 ELISA 검측대서혈장거갑신상선소(NA)、내피형일양화담합매(eNOS)화내피소-1(ET-1)수평。결과：여정상대조조비교,PTSD 조대서재광장중천행격수급직립차수현저감소(P <0.01)；장계막급연뇌막세동맥、세정맥관경명현변세(P <0.05),혈류속도추완；혈장 NA 화 ET-1함량현저승고(P <0.01),단 eNOS 수평무현저변화(P >0.05)。결론：PTSD 대서존재장계막화연뇌막미순배장애。
Objective:To investigate the effect of post-traumatic stress disorder (PTSD)on microcirculation of mesentery and cerebral pia mater in rats and the underlying mechanisms.Method:Sprague-Dawley (SD)rats were randomly divided into two groups:control group (n=6)and PTSD group (n=12).The PTSD model was rep-licated by using the single prolonged stress method.On the 14th day post-stress,the changes of animals'behavior was evaluated via open-field;the microcirculation of mesentery and cerebral pia mater of rats was observed using the microscope;the plasma noradrenaline (NA),endothelial nitric oxide synthase (eNOS)and endothelin-1 (ET-1 ) were measured by using enzyme linked immunosorbent assay (ELISA).Results:Compared with the control rats,the numbers of crossing and rearing of the PTSD rats were decreased significantly in the open-field (P <0.01);the di-ameters of arteriole and veinule in the mesentery and cerebral pia mater from the PTSD rats were significantly thin-ner (P <0.05),the blood flow velocity showed the trend of slowed down;the plasma levels of NA and ET-1 were elevated significantly (P <0.01);there was no significant difference in the plasma level of eNOS (P >0.05).Con-clusion:PTSD induces microcirculation dysfunction in mesentery and cerebral pia mater in rats.
