CAJ | 학술논문

目的：：确定特应性皮炎( AD)患儿外周血CD4＋T细胞中miR－155的表达与疾病严重程度的相关性。方法：采用实时荧光定量聚合酶链反应( Real－time－PCR)检测45例AD患儿和38例健康者对照外周血CD4＋T细胞中miR－155的表达,ELISA法检测血清IL－5和IFN－γ表达,并进行SCO-RAD评分。结果： AD组miR－155的表达较对照组明显升高( P<0.05)；AD患者组miR－155的表达与SCORAD评分、血清IL－5表达水平呈正相关(均P<0.05),与血清IFN－γ表达水平呈负相关(P<0.05)。结论： AD患儿外周血CD4＋T细胞中miR－155表达与疾病严重程度正相关,可作为评定AD严重程度的一个指标。
목적：：학정특응성피염( AD)환인외주혈CD4＋T세포중miR－155적표체여질병엄중정도적상관성。방법：채용실시형광정량취합매련반응( Real－time－PCR)검측45례AD환인화38례건강자대조외주혈CD4＋T세포중miR－155적표체,ELISA법검측혈청IL－5화IFN－γ표체,병진행SCO-RAD평분。결과： AD조miR－155적표체교대조조명현승고( P<0.05)；AD환자조miR－155적표체여SCORAD평분、혈청IL－5표체수평정정상관(균P<0.05),여혈청IFN－γ표체수평정부상관(P<0.05)。결론： AD환인외주혈CD4＋T세포중miR－155표체여질병엄중정도정상관,가작위평정AD엄중정도적일개지표。
Objective:To determine the correlation of miR-155 in the peripheral blood CD4+T cells from children with atopic dermatitis ( AD) with disease severity. Methods:The expression levels of miR-155 of 45 children with AD and 38 healthy controls were detected by Real time-PCR. The levels of serum IL-5 and IFN-γ were tested by ELISA. SCORAD of the patients were assessed. Results:The expression level of miR-155 of children with AD was higher than that in healthy controls ( P<0.05) . The expression level of miR-155 was positively correlated with the SCORAD and serum IL-5 level ( P<0.05) and negatively correlated with serum IFN-γ (P<0.05). Conclusion: miR-155 is positively correlated with the disease severity in children with AD, which can be used for a marker of disease severity in AD.