CAJ | 학술논문

以番茄籽为原料提取番茄籽油，通过响应面法优化微波辅助复合酶法提取番茄籽油（MEOs）工艺，并建立回归模型；同时研究了 MEOs 的理化特性、脂肪酸组成和抗氧化活性，并与传统索氏提取番茄籽油（SEOs）进行比较。结果表明，采用复合酶（纤维素酶∶果胶酶∶蛋白酶＝1∶1∶1）的最佳提取工艺为酶添加量1．5％、温度35℃、时间40 min、微波功率500 W，在此条件下 MEOs 得率为29．65％；MEOs 与 SEOs 在理化特性方面没有显著区别，但 MEOs 含有较高的生育酚，同时具有较高的氧化稳定性。MEOs 表现出较强的抗氧化活性，在 DPPH·和β－胡萝卜素－亚油酸测试体系中其 IC50分别为9．52和10．64 mg／mL。MEOs 是一种理想的天然保健食品原料，具有较好的开发利用前景。
이번가자위원료제취번가자유，통과향응면법우화미파보조복합매법제취번가자유（MEOs）공예，병건립회귀모형；동시연구료 MEOs 적이화특성、지방산조성화항양화활성，병여전통색씨제취번가자유（SEOs）진행비교。결과표명，채용복합매（섬유소매∶과효매∶단백매＝1∶1∶1）적최가제취공예위매첨가량1．5％、온도35℃、시간40 min、미파공솔500 W，재차조건하 MEOs 득솔위29．65％；MEOs 여 SEOs 재이화특성방면몰유현저구별，단 MEOs 함유교고적생육분，동시구유교고적양화은정성。MEOs 표현출교강적항양화활성，재 DPPH·화β－호라복소－아유산측시체계중기 IC50분별위9．52화10．64 mg／mL。MEOs 시일충이상적천연보건식품원료，구유교호적개발이용전경。
Response surface methodology has been applied to optimize the microwave -assisted aqueous enzy-matic extraction of oil from tomato seeds (MEOs).The physicalchemical compositions,fatty acid compositions and antioxidant activities of MEOs have been compared with that features of soxhlet -extracted oil (SEOs).The optimal extraction conditions was found to have enzyme concentration of 1.5% (cellulase,pectinase and proteinase:1∶1∶1), temperature of 35 ℃,time of 40 min and irradiation power of 500 W.On the conditions,the extraction yield of MEOs could reach 29.65%.Moreover,there were no significant variations in physicochemical properties of MEOs and SEOs,while MEOs had the higher tocopherols and exhibited the better oxidation stability.MEOs showed the stronger antioxidant activities with IC50 values of 9.52 and 10.64 mg/mL according to DPPH radical scavenging assay and β-carotene /linoleic acid bleaching test.MEOs shall be a fine nutritional material which has wide prospects in health food fields.