CAJ | 학술논문

为评价超微粉碎对苦荞多酚及抗氧化活性的影响，分别采用 NaNO2－Al （NO3）3法、Folin －Ciocalteu法与高效液相色谱法测定不同粒径苦荞微粉中黄酮、酚与芦丁的含量，DPPH 自由基清除法、ABTS ＋·清除法、总抗氧化能力检测试剂盒评价其抗氧化能力，并分析了酚、黄酮、芦丁含量与抗氧化能力之间的相关性。结果表明：随着微粉化程度的增加，黄酮、酚、芦丁含量及抗氧化能力均呈现先减弱后增强的趋势，但减弱幅度较小，且微粉化程度较高时能较好的保留与提高多酚含量及增加抗氧化活性，对结合态芦丁含量影响不显著；苦荞粉中酚、黄酮、芦丁含量与抗氧化能力之间具有显著的相关性（P ＜0．01）。超微粉碎是一种有效提升苦荞加工食用品质，释放苦荞多酚类植物活性成分的较好加工方式。
위평개초미분쇄대고교다분급항양화활성적영향，분별채용 NaNO2－Al （NO3）3법、Folin －Ciocalteu법여고효액상색보법측정불동립경고교미분중황동、분여호정적함량，DPPH 자유기청제법、ABTS ＋·청제법、총항양화능력검측시제합평개기항양화능력，병분석료분、황동、호정함량여항양화능력지간적상관성。결과표명：수착미분화정도적증가，황동、분、호정함량급항양화능력균정현선감약후증강적추세，단감약폭도교소，차미분화정도교고시능교호적보류여제고다분함량급증가항양화활성，대결합태호정함량영향불현저；고교분중분、황동、호정함량여항양화능력지간구유현저적상관성（P ＜0．01）。초미분쇄시일충유효제승고교가공식용품질，석방고교다분류식물활성성분적교호가공방식。
To evaluate the influence of polyphenols and antioxidant activity of buckwheat after micronization.. Flavonoids content,phenol content and rutin content with different particle size was determined by NaNO2 -Al (NO3 )3 colorimetry,Folin -Ciocalteu method and HPLC respectively,Antioxidative activity was evaluated by DP-PH,ABTS +· scavenging activity method and total antioxidant capacity assay kit;the correlation between total phe-nols,flavonoids and antioxidant capacity has also been analyzed.The results showed that the contents of phenolics, flavonoids and rutin and antioxidant activity were all first decreased and then increased along with the increase of de-gree of micronization,while they could be weakened to a lesser extent;holding a higher degree of micronization could better retain and increase the content of polyphenol and antioxidant activity,but the bound rutin could not be affected by micronization.Total phenol and flavonoids content in buckwheat had significant correlation with antioxidant capac-ity (P <0.01).Micronization was an effective method to promote the processing quality,and release polyphenolic phytochemicals of buckwheat.