福建医科大学学报
福建醫科大學學報
복건의과대학학보
Journal of Fujian Medical University
2015年
4期
207-210
,共4页
乙型肝炎病毒%基因型%熔解曲线法%肝纤维化%HBV DNA
乙型肝炎病毒%基因型%鎔解麯線法%肝纖維化%HBV DNA
을형간염병독%기인형%용해곡선법%간섬유화%HBV DNA
hepatitis B virus%genotype%melting curve analysis%liver fibrosis%HBV DNA
目的：分析福州市乙型肝炎病毒(HBV)感染者病毒基因型分布情况及其与部分临床指标的关系。方法收集346份 HBV DNA 阳性标本,利用 PCR 熔解曲线法检测其基因型,同时进行 HBeAg 定量的检测,其中91例经肝脏穿刺确诊为肝纤维化。根据分型结果探讨基因型与肝纤维化、HBeAg 阳性率以及 HBV DNA 水平的关系。结果346例 HBV 感染者中,检出 B 型193例,占55.78%；检出 C 型95例,占27.46%；检出 B/C 混合型58例,占16.76%。91例肝纤维化标本中,检出 C 型39例,占42.86%(39/91),显著高于 C 型在全部研究对象中所占的比例(27.46%,82/346)；检出 B 型38例,占41.76%(38/91),低于 B 型在全部研究对象中所占的比例(55.78%,193/346)。对346例标本进行 HBV DNA 定量检测,发现 C 型的病毒载量明显高于 B 型和 B/C 混合型；HBeAg 定量检测发现 C 型的 HBeAg 阳性率(87.37%,83/95)亦显著高于 B 型(68.39%,132/193)和 B/C 混合型(41.38%,24/58),差别均有统计学意义(P <0.05)。结论福州市乙型肝炎患者中以 B 型 HBV 感染为主；肝纤维化、HBeAg 阳性率及 HBV DNA 病毒载量与乙型肝炎患者感染 HBV 的基因型相关；C 型与肝纤维化有关。
目的：分析福州市乙型肝炎病毒(HBV)感染者病毒基因型分佈情況及其與部分臨床指標的關繫。方法收集346份 HBV DNA 暘性標本,利用 PCR 鎔解麯線法檢測其基因型,同時進行 HBeAg 定量的檢測,其中91例經肝髒穿刺確診為肝纖維化。根據分型結果探討基因型與肝纖維化、HBeAg 暘性率以及 HBV DNA 水平的關繫。結果346例 HBV 感染者中,檢齣 B 型193例,佔55.78%；檢齣 C 型95例,佔27.46%；檢齣 B/C 混閤型58例,佔16.76%。91例肝纖維化標本中,檢齣 C 型39例,佔42.86%(39/91),顯著高于 C 型在全部研究對象中所佔的比例(27.46%,82/346)；檢齣 B 型38例,佔41.76%(38/91),低于 B 型在全部研究對象中所佔的比例(55.78%,193/346)。對346例標本進行 HBV DNA 定量檢測,髮現 C 型的病毒載量明顯高于 B 型和 B/C 混閤型；HBeAg 定量檢測髮現 C 型的 HBeAg 暘性率(87.37%,83/95)亦顯著高于 B 型(68.39%,132/193)和 B/C 混閤型(41.38%,24/58),差彆均有統計學意義(P <0.05)。結論福州市乙型肝炎患者中以 B 型 HBV 感染為主；肝纖維化、HBeAg 暘性率及 HBV DNA 病毒載量與乙型肝炎患者感染 HBV 的基因型相關；C 型與肝纖維化有關。
목적：분석복주시을형간염병독(HBV)감염자병독기인형분포정황급기여부분림상지표적관계。방법수집346빈 HBV DNA 양성표본,이용 PCR 용해곡선법검측기기인형,동시진행 HBeAg 정량적검측,기중91례경간장천자학진위간섬유화。근거분형결과탐토기인형여간섬유화、HBeAg 양성솔이급 HBV DNA 수평적관계。결과346례 HBV 감염자중,검출 B 형193례,점55.78%；검출 C 형95례,점27.46%；검출 B/C 혼합형58례,점16.76%。91례간섬유화표본중,검출 C 형39례,점42.86%(39/91),현저고우 C 형재전부연구대상중소점적비례(27.46%,82/346)；검출 B 형38례,점41.76%(38/91),저우 B 형재전부연구대상중소점적비례(55.78%,193/346)。대346례표본진행 HBV DNA 정량검측,발현 C 형적병독재량명현고우 B 형화 B/C 혼합형；HBeAg 정량검측발현 C 형적 HBeAg 양성솔(87.37%,83/95)역현저고우 B 형(68.39%,132/193)화 B/C 혼합형(41.38%,24/58),차별균유통계학의의(P <0.05)。결론복주시을형간염환자중이 B 형 HBV 감염위주；간섬유화、HBeAg 양성솔급 HBV DNA 병독재량여을형간염환자감염 HBV 적기인형상관；C 형여간섬유화유관。
Objective To understand the distribution of HBV genotype in Fuzhou,and investigate the association between HBV genotype and liver fibrosis,HBeAg and HBV DNA. Methods 346 sam-ples were genotyped by PCR melt curve analysis;all of them were HBV DNA≥10 3 copies/mL and HBeAg are detected. 91 of them were diagnosed as liver fibrosis by liver biopsy. Based on the laboratory re-sults we analysed the relationship between HBV genotypes with liver fibrosis,HBeAg positive incidence, and HBV DNA level. Results HBV genotype B,C and genotype B/C were detected in 346 serum sam-ples,at rate of 55.78%(1 93/346),27.46%(95/346),1 6.76%(58/346)of total number of specimens re-spectively. Of the 91 samples that were diagnosed as liver fibrosis,39 samples were with genotype C,ac-counting for 42.86%(39/91 ),which was higher than the proportion(27.46%)of genotype C among the 346 samples. 38 samples were genotype B,accounting for 41.76%(38/91 ),which was lower than the proportion(55.78%)of genotype B among the 346 samples. All of 346 samples were tested for HBV DNA. The HBV DNA detetion results showed that the HBV DNA of genotype C is higher significantly than that of genotype B and B/C(P <0.05),the HBeAg positive rate of genotype C(87.37%)is higher sig-nificantly than that of genotype B(68.39%)and B/C(41.38%)(P <0.05). Conclusion The main geno-type of HBV is B in Fuzhou population,the genotype of HBV is associated withto liver fibrosis,HBeAg positive incidence,and HBV DNA level. Genotype C is closely associated with the severity of liver dis-ease.
