CAJ | 학술논문

目的：检测B7家族在非霍奇金淋巴瘤（ NHL）中的表达，阐明其在NHL中的作用及意义。方法应用密度梯度离心法分离健康志愿者外周血单个核细胞（ PBMCs），实时荧光定量PCR（ RQ-PCR）检测B7家族PD-L1和PD-L2 mRNA在B细胞淋巴瘤（B-NHL）、T细胞淋巴瘤（T-NHL）及NK/T细胞淋巴瘤（NKTL）细胞系中的表达水平。结果 PD-L1和PD-L2 mRNA在NHL各细胞系中呈不同程度表达，T-NHL及NKTL细胞系中其表达量均明显高于正常PBMCs（P＜0.05），而B-NHL细胞系中PD-L1和PD-L2 mRNA表达量与正常PBMCs相比无明显差异（ P＞0.05）。结论在NHL中， B7家族中PD-L1及PD-L2可能参与了NKTL及T-NHL肿瘤的免疫逃逸，有望成为NKTL及T-NHL肿瘤免疫治疗的新靶点。
목적：검측B7가족재비곽기금림파류（ NHL）중적표체，천명기재NHL중적작용급의의。방법응용밀도제도리심법분리건강지원자외주혈단개핵세포（ PBMCs），실시형광정량PCR（ RQ-PCR）검측B7가족PD-L1화PD-L2 mRNA재B세포림파류（B-NHL）、T세포림파류（T-NHL）급NK/T세포림파류（NKTL）세포계중적표체수평。결과 PD-L1화PD-L2 mRNA재NHL각세포계중정불동정도표체，T-NHL급NKTL세포계중기표체량균명현고우정상PBMCs（P＜0.05），이B-NHL세포계중PD-L1화PD-L2 mRNA표체량여정상PBMCs상비무명현차이（ P＞0.05）。결론재NHL중， B7가족중PD-L1급PD-L2가능삼여료NKTL급T-NHL종류적면역도일，유망성위NKTL급T-NHL종류면역치료적신파점。
ObjectiveTo detect the expression of B7 family molecules and illuminatetheir significance innon-Hodgkin’s lymphoma (NHL).Methods Peripheralbloodmonocytecells (PBMCs) were separated by density gradient centrifugation from peripheral blood samples of healthy volunteers. Real-time fluorescence quantitative PCR (RQ-PCR) was used to determine the mRNA expression levels of PD-L1 and PD-L2 in the cell lines of B cell lymphoma, T cell lymphoma and NK/T cell lymphoma (NKTL).Results PD-L1 and PD-L2 were expressed in NHL cell lines at different mRNA levels. Their expression was significantly higher in T cell lymphoma and NKTL cell lines thaninnormal PBMCs (P<0.05). However, there was no significant difference in theirexpression between B cell lymphoma cell lines and PBMCs (P>0.05).Conclusion B7 family molecules such as PD-L1 and PD-L2 may be involved in the tumor immune escape in NKTL and T cell lymphoma, and they might be novel targets for NHL immunotherapy.