CAJ | 학술논문

基于1H NMR的代谢组学方法对灌胃给药赭石的成年Wistar大鼠血清进行分析，运用主成分分析模式识别方法对所得数据进行处理，并对给药大鼠血清进行生化指标检测．研究结果表明，大鼠体内?-羟异丁酸、乙酸、丙酮、胆碱、甘油磷脂酰胆碱、葡萄糖、乳酸、低密度脂蛋白、极低密度脂蛋白和脂质等内源性代谢物浓度发生明显变化，可作为赭石的特征代谢物．2 g/kg和5 g/kg体重剂量赭石使大鼠机体产生大量活性氧化物(ROS)，造成过氧化损伤，导致能量代谢和糖代谢紊乱，糖酵解反应增强，并且对肝功能造成了影响．
기우1H NMR적대사조학방법대관위급약자석적성년Wistar대서혈청진행분석，운용주성분분석모식식별방법대소득수거진행처리，병대급약대서혈청진행생화지표검측．연구결과표명，대서체내?-간이정산、을산、병동、담감、감유린지선담감、포도당、유산、저밀도지단백、겁저밀도지단백화지질등내원성대사물농도발생명현변화，가작위자석적특정대사물．2 g/kg화5 g/kg체중제량자석사대서궤체산생대량활성양화물(ROS)，조성과양화손상，도치능량대사화당대사문란，당효해반응증강，병차대간공능조성료영향．
Wistar rats were intragastrically administered with different doses (0, 2, 5 g/kg body weight) of haematitum.1H NMR-based metabonomic techniques were used to analyze the metabolic profiles of the serum samples collected from the treated rats. Significant treatment-related changes were observed for the levels of?-hydroxybutyate, acetate, acetone, choline, glycerophosphorylcholine, glucose, lactate, low density lipoprotein, very low density lipoprotein and lipids. Such biochemical changes indicated that haematitum treatment at the dose of 2 or 5 g/kg body weight gave rise to reactive oxygen species (ROS) and caused peroxidation damage. It is concluded that haematitum treatment affects glucose, lipid and energy metabolism in rats.