当代临床医刊
噹代臨床醫刊
당대림상의간
The Medical Journal of Thepresent Clinical
2015年
4期
1477-1478,1479
,共3页
钙敏感受体%扩张型心肌病%阿霉素%大鼠
鈣敏感受體%擴張型心肌病%阿黴素%大鼠
개민감수체%확장형심기병%아매소%대서
calcium-sensing receptors%dilated cardiomyopathy%doxorubicin%rats
目的：观察阿霉素（doxorubicin ，DOX）诱导大鼠扩张型心肌病（ dilated cardiomyopathy ，DCM）钙敏感受体（calcium－sensing receptor， CaSR）表达的变化，探讨钙敏感受体在扩张型心肌病中的作用。方法40只雄性Wistar大鼠，随机分为对照组（n＝10）和模型组（n ＝30），模型组大鼠分为3周组、6周组和9周组。采用腹腔注射阿霉素（25．mg · kg－1· w－1）建立大鼠DCM模型。应用超声心动图检测各组大鼠左心室内径以及心功能。 HE染色观察大鼠心肌超微结构变化。 Western blot和免疫组化法检测心肌组织CaSR蛋白的表达情况。结果（1）与对照组相比，9周组大鼠左室舒张末期内径（LVDD）明显增加（P ＜0．01），左室射血分数（LVEF）明显降低，；（2）随着病程延长模型组大鼠心肌CaSR表达明显降低（P＜0．01）。结论在大鼠扩张型心肌病的发生过程中， CaSR 表达逐渐降低，可能导致钙稳态紊乱参与DCM的发生。
目的：觀察阿黴素（doxorubicin ，DOX）誘導大鼠擴張型心肌病（ dilated cardiomyopathy ，DCM）鈣敏感受體（calcium－sensing receptor， CaSR）錶達的變化，探討鈣敏感受體在擴張型心肌病中的作用。方法40隻雄性Wistar大鼠，隨機分為對照組（n＝10）和模型組（n ＝30），模型組大鼠分為3週組、6週組和9週組。採用腹腔註射阿黴素（25．mg · kg－1· w－1）建立大鼠DCM模型。應用超聲心動圖檢測各組大鼠左心室內徑以及心功能。 HE染色觀察大鼠心肌超微結構變化。 Western blot和免疫組化法檢測心肌組織CaSR蛋白的錶達情況。結果（1）與對照組相比，9週組大鼠左室舒張末期內徑（LVDD）明顯增加（P ＜0．01），左室射血分數（LVEF）明顯降低，；（2）隨著病程延長模型組大鼠心肌CaSR錶達明顯降低（P＜0．01）。結論在大鼠擴張型心肌病的髮生過程中， CaSR 錶達逐漸降低，可能導緻鈣穩態紊亂參與DCM的髮生。
목적：관찰아매소（doxorubicin ，DOX）유도대서확장형심기병（ dilated cardiomyopathy ，DCM）개민감수체（calcium－sensing receptor， CaSR）표체적변화，탐토개민감수체재확장형심기병중적작용。방법40지웅성Wistar대서，수궤분위대조조（n＝10）화모형조（n ＝30），모형조대서분위3주조、6주조화9주조。채용복강주사아매소（25．mg · kg－1· w－1）건립대서DCM모형。응용초성심동도검측각조대서좌심실내경이급심공능。 HE염색관찰대서심기초미결구변화。 Western blot화면역조화법검측심기조직CaSR단백적표체정황。결과（1）여대조조상비，9주조대서좌실서장말기내경（LVDD）명현증가（P ＜0．01），좌실사혈분수（LVEF）명현강저，；（2）수착병정연장모형조대서심기CaSR표체명현강저（P＜0．01）。결론재대서확장형심기병적발생과정중， CaSR 표체축점강저，가능도치개은태문란삼여DCM적발생。
Objce tive observation of doxorubicin (doxorubicin,DOX) in rats induced by dilated cardiomyopathy (dilated car-diomyopathy, DCM) calcium-sensing receptor (calcium-sensing receptor, CaSR)the expression change of calcium -sensing re-ceptor in the function of dilated cardiomyopathy.Methods Forty male Wistar rats were randomly divided into two groups (n=10) and the model group (n =30), models of rats were divided into 3 weeks, 6 weeks and 9weeks group .By intraperitoneal injection of ADRIAMYCIN (2.5mg · kg-1 · w-1 ) establishing rat model of DCM.Echocardiography in detecting left ventricular diameter as well as cardiac function in each group.HE staining observation of changes of myocardial ultrastructure in rats.Western blot and Im-munohistochemistry assay for detection of myocardial tissue expression of CaSR.Resu lts compared with the control group,9-week rats left ventricular end-diastolic diameter (LVDD) were significantly increased (p <0.01), left ventricular ejection fraction (LVEF) was significantly reduced, second as duration model of rat myocardial CaSR expression significantly reduced (P<0.01). Findings in rats with dilated cardiomyopathy during CaSR expression decreases , which might lead to disturbance of Calcium Homeo-stasis in participating in DCM.Enter text you want to translate!
