CAJ | 학술논문

该文应用核磁共振（NMR）和高效液相色谱-质谱（HPLC-MS）技术系统分析了牡丹不同根部位的代谢物组成，共鉴定了包括蔗糖、苯乙酰类（4种）、酚酸类（6种）、单萜苷类（2种）、黄酮类（1种）和不饱和脂肪酸（2种）共16种代谢物。在牡丹根中，丹皮酚以及丹皮酚苷等苯乙酰类成分和蔗糖的含量较高，而酚酸、黄酮和单萜苷类成分的含量较低。基于 NMR 的代谢组分析表明，牡丹不同根部位的代谢物组成具有明显差异：在二级侧根、一级侧根和主根中，丹皮酚及丹皮酚苷类的含量依次较高而蔗糖含量依次降低，这可能与不同根部位的植物生理有关；和侧根相比，主根中具有活性的代谢物含量较高，推测主根的品质优于侧根，这为市场上牡丹根皮的等级划分提供了重要的科学依据。
해문응용핵자공진（NMR）화고효액상색보-질보（HPLC-MS）기술계통분석료모단불동근부위적대사물조성，공감정료포괄자당、분을선류（4충）、분산류（6충）、단첩감류（2충）、황동류（1충）화불포화지방산（2충）공16충대사물。재모단근중，단피분이급단피분감등분을선류성분화자당적함량교고，이분산、황동화단첩감류성분적함량교저。기우 NMR 적대사조분석표명，모단불동근부위적대사물조성구유명현차이：재이급측근、일급측근화주근중，단피분급단피분감류적함량의차교고이자당함량의차강저，저가능여불동근부위적식물생리유관；화측근상비，주근중구유활성적대사물함량교고，추측주근적품질우우측근，저위시장상모단근피적등급화분제공료중요적과학의거。
The distribution of metabolites in the root bark of tree peony from different root parts was systematically analyzed using the combination of NMR and HPLC-MS for the first time. A total of 16 metabolites from their methanol extracts were simultaneously identified and quantified, including 1 primary metabolite (sucrose) and 15 secondary ones such as 4 acetophenones, 6 phenolics, 2 monoterpene glycosides, 1 flavonoid and 2 unsaturated fatty acids. The quantitative data indicated that there are the higher levels of acetophenones and sucrose, and the lower contents of phenolics, monoterpene glycosides, flavonoids and unsaturated fatty acids in the tree peony roots. NMR-based metabolomics revealed that the metabolite compositions were found to be different among the three root parts. The increasing levels of acetophenones and decreasing level of sucrose were observed in second lateral root, the first lateral and axial root in order. The axial roots have higher quality than the lateral roots in tree peony due to the accumulation of bioactive secondary metabolites associated with plant physiology. These findings provided important scientific evidence for grading the root bark of tree peony on the general market.