CAJ | 학술논문

目的：观察四神丸对脾肾阳虚型溃疡性结肠炎大鼠中血清转化生长因子β1（ TGFβ1）、白介素6（IL－6）及结肠组织中Toll样受体4（TLR－4） mRNA含量的变化，探讨四神丸对脾肾阳虚型溃疡性结肠炎的作用机制。方法：采用番泻叶灌胃＋氢化可的松注射液腹腔注射＋2、4、6－三硝基苯磺酸（ TN-BS）／乙醇法灌肠建立脾肾阳虚型溃疡性结肠炎大鼠模型。将60只wistar大鼠随机分为空白组、模型组、四神丸组及柳氮磺嘧啶（ SASP ）组，经药物干预后分别采用酶联免疫法检测各组大鼠血清中的TGFβ1、IL－6含量及半定量反转录－聚合酶链反应检测各组大鼠结肠组织中的TLR－4 mRNA含量。结果：与空白组比较，模型组大鼠中TGFβ1含量降低（P＜0．05），IL－6含量升高（P＜0．05）；与模型组比较，各治疗组大鼠中TGFβ1含量升高（P＜0．05），IL－6含量降低（P＜0．05）。与空白组比较，模型组大鼠结肠组织TLR－4 mRNA表达显著增强（P＜0．05）；各治疗组TLR－4 mRNA表达减弱（P＜0．05）。结论：四神丸能够通过下调IL－6和升高TGFβ1的表达，降低结肠组织中TLR－4 mRNA的含量，抑制炎症反应，调节肠上皮免疫系统，恢复肠粘膜微环境的免疫稳态，达到治疗UC的目的。
목적：관찰사신환대비신양허형궤양성결장염대서중혈청전화생장인자β1（ TGFβ1）、백개소6（IL－6）급결장조직중Toll양수체4（TLR－4） mRNA함량적변화，탐토사신환대비신양허형궤양성결장염적작용궤제。방법：채용번사협관위＋경화가적송주사액복강주사＋2、4、6－삼초기분광산（ TN-BS）／을순법관장건립비신양허형궤양성결장염대서모형。장60지wistar대서수궤분위공백조、모형조、사신환조급류담광밀정（ SASP ）조，경약물간예후분별채용매련면역법검측각조대서혈청중적TGFβ1、IL－6함량급반정량반전록－취합매련반응검측각조대서결장조직중적TLR－4 mRNA함량。결과：여공백조비교，모형조대서중TGFβ1함량강저（P＜0．05），IL－6함량승고（P＜0．05）；여모형조비교，각치료조대서중TGFβ1함량승고（P＜0．05），IL－6함량강저（P＜0．05）。여공백조비교，모형조대서결장조직TLR－4 mRNA표체현저증강（P＜0．05）；각치료조TLR－4 mRNA표체감약（P＜0．05）。결론：사신환능구통과하조IL－6화승고TGFβ1적표체，강저결장조직중TLR－4 mRNA적함량，억제염증반응，조절장상피면역계통，회복장점막미배경적면역은태，체도치료UC적목적。
Objective:To observe the influence of Sishen pill on serum of transforming growth factor beta 1 (TGFβ1), in-terleukin 6 (IL-6) and colonic tissue of toll -like receptor 4 (TLR-4) mRNA content changes and identify its under-lying mechanism of action .Methods:Use lavage of senna +intraperitoneal injection of hydrocortisone injection +enema of 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)/ethanol to establish the rat models of ulcerative colitis with spleen -kidney deficiency type .60 wistar rats were randomly divided into a blank group ,a model group , a Sishen pill group and a salazosulfadimidine (SASP) group.After drug intervention, serums of TGF β1 and IL-6 were detected by ELISA, and the colonic tissue of TNF -αmRNA was detected by Sq RT -PCR.Results: Compared with those in the blank group,in the model group rats’ TGFβ1 was decreased (P<0.05),but IL-6 content was increased (P<0.05);com-pared with those in the model group ,in the treatment group TGF β1 content was increased (P<0.05),but IL-6 was decreased (P<0.05).Compared with that in the blank group , the expression of the model group ’s TLR-4 mRNA of colonic tissue was significantly enhanced (P<0.05);the expression of each treatment group ’s TLR-4 mRNA was de-creased (P<0.05).Conclusion:Sishen pill can decrease the expression of IL -6, increase the expression of TGF β1, reduce content of TLR-4 mRNA in colonic tissue ,and then it can inhibit the reaction of inflammation , regulate intesti-nal epithelial immune system , restore intestinal mucosal immune steady -state microenvironment , then achieve the pur-pose of the treatment of UC .