CAJ | 학술논문

目的：本实验以Ⅱ型胶原诱导的类风湿关节炎大鼠（ CIA）为模型，通过观察电针对RA模型大鼠VEGF表达的影响，探讨电针治疗类风湿性关节炎的作用机理。方法：60只雄性的Wistar大鼠，随机取8只为正常组，其余大鼠采用Ⅱ型胶原和弗氏不完全佐剂复制类风湿性关节炎模型。造模后，从造模成功的大鼠中取24只大鼠随机分为模型组、泼尼松组和电针组，每组8只。造模后第21天开始治疗，正常组和模型组用生理盐水（0．1mL／10g）灌胃，泼尼松组用泼尼松药液（0．1mL／10g）灌胃，电针组用0.30cm ×25mm的针灸针针刺大鼠双侧的“足三里”和“昆仑”穴，强度为1～2mA，频率2／100Hz，每次30 min，每日1次，连续治疗10次。分别在造模前、造模第7天、造模第14天、造模第21天和治疗结束后称取大鼠的体质量，并采用ELISA法和蛋白印迹法（ WB ）检测血清和膝关节组织内VEGF的含量。结果：造模后的大鼠可见食量减少，体质量下降，足爪红肿，双侧踝关节肿胀，活动受限；与正常组相比，模型组大鼠的体质量增长变缓（P＜0．05），电针组和泼尼松组与模型组相比差异不明显（P＞0．05）；与正常组相比，模型组血清和膝关节内的VEGF含量均升高（ P＜0．05）；与模型组相比，经电针和泼尼松治疗后的大鼠血清和膝关节内的VEGF含量均将低（ P＜0．05）；电针组与泼尼松组相比，差异不明显（P＞0．05）。结论：（1）电针可以显著改善CIA大鼠的饮食状况，增加体质量，减轻关节肿胀；（2）电针可以减少VEGF的分泌可能就是其中的机制之一。
목적：본실험이Ⅱ형효원유도적류풍습관절염대서（ CIA）위모형，통과관찰전침대RA모형대서VEGF표체적영향，탐토전침치료류풍습성관절염적작용궤리。방법：60지웅성적Wistar대서，수궤취8지위정상조，기여대서채용Ⅱ형효원화불씨불완전좌제복제류풍습성관절염모형。조모후，종조모성공적대서중취24지대서수궤분위모형조、발니송조화전침조，매조8지。조모후제21천개시치료，정상조화모형조용생리염수（0．1mL／10g）관위，발니송조용발니송약액（0．1mL／10g）관위，전침조용0.30cm ×25mm적침구침침자대서쌍측적“족삼리”화“곤륜”혈，강도위1～2mA，빈솔2／100Hz，매차30 min，매일1차，련속치료10차。분별재조모전、조모제7천、조모제14천、조모제21천화치료결속후칭취대서적체질량，병채용ELISA법화단백인적법（ WB ）검측혈청화슬관절조직내VEGF적함량。결과：조모후적대서가견식량감소，체질량하강，족조홍종，쌍측과관절종창，활동수한；여정상조상비，모형조대서적체질량증장변완（P＜0．05），전침조화발니송조여모형조상비차이불명현（P＞0．05）；여정상조상비，모형조혈청화슬관절내적VEGF함량균승고（ P＜0．05）；여모형조상비，경전침화발니송치료후적대서혈청화슬관절내적VEGF함량균장저（ P＜0．05）；전침조여발니송조상비，차이불명현（P＞0．05）。결론：（1）전침가이현저개선CIA대서적음식상황，증가체질량，감경관절종창；（2）전침가이감소VEGF적분비가능취시기중적궤제지일。
Objective:Autoimmune collagen type II-induced arthritis ( CIA ) rat models were used to observe the influ-ence of electro-acupuncture(EA) on VEGF so as to study its underlying mechanism in improving RA .Methods:There were 60 male Wistar rats and randomly choose 8 as normal control group , and the rest were used by infreund ’ s adjuvant and type Ⅱ collagen to induce arthritis .24 rats which were successfully induced were randomly divided into three groups:model group, prednisolone group and electro -acupuncture(EA) group,with 8 in eaeh group.Treatment from the 21st day ,the normal group and model group were given saline solution (0.1mL/10g) to fill the stomach, and pred-nisone group was given prednisone solution (0.1mL/10g) to fill the stomach.The EA(2/100Hz,1~2mA) group was applied to “Zusanli”(ST36) and “Kunlun”(BL60) for 30min, once daily for 10 times.Measure the rats'body weight before and after the establishment , and at the end of the treatment .Then determine the plasma VEGF contents in serum and the knee joint cavity by ELISA and Western blot .Results:After modeling ,paw swelling ,ankle swelling ,food volume and weight had different degrees of reduction;in comparison with rats in the control group , the rats in model group gained weight slowly (P<0.05);compared with model group , the prednisolone group and electro -acupuncture group had no significant difference ( P>0.05);in comparison with the normal group , serum VEGF levels within the knee were increased ( P<0 .05 ); in comparison with the model group , the serum VEGF levels within the knee joint increased markedly after treatment ( P<0 .05 ) , while the prednisolone group and electro -acupuncture group had no obvious difference(P>0.05).Conclusion:(1)EA can improve the CIA rats'diet and joint swelling degree significantly , then has an obviously therapeutic effect on CIA rats;(2)The decreased expression of VEGF may be one of the mechanisms .