CAJ | 학술논문

梅毒的诊断主要依据临床表现,血清学检测和梅毒螺旋体暗视野检查.随着人们对梅毒螺旋体基因组的认识,已经建立了多种基于梅毒螺旋体特异性基因序列的聚合酶链反应检测方法,常规聚合酶链反应、巢式聚合酶链反应、反转录聚合酶链反应、多重聚合酶链反应和实时荧光聚合酶链反应可以检测多种不同类型的临床标本,用于梅毒螺旋体的基因检测.聚合酶链反应扩增的靶基因有tpp47、polA、bmp、tmpA、arp、tpr等,其中tpp47、polA为常用的靶基因.皮损拭子、血液、脑脊液、病理组织、羊水等均可进行聚合酶链反应检测.
매독적진단주요의거림상표현,혈청학검측화매독라선체암시야검사.수착인문대매독라선체기인조적인식,이경건립료다충기우매독라선체특이성기인서렬적취합매련반응검측방법,상규취합매련반응、소식취합매련반응、반전록취합매련반응、다중취합매련반응화실시형광취합매련반응가이검측다충불동류형적림상표본,용우매독라선체적기인검측.취합매련반응확증적파기인유tpp47、polA、bmp、tmpA、arp、tpr등,기중tpp47、polA위상용적파기인.피손식자、혈액、뇌척액、병리조직、양수등균가진행취합매련반응검측.
The diagnosis of syphilis is mainly based on clinical manifestations,serological analysis,and dark-field microscopy for detection of Treponema pallidum.With further clarification of the genomic sequence of Treponema pallidum,several PCR methods based on Treponema pallidum-specific gene sequences have been developed during the last 20 years.Classical PCR,nested-PCR,reverse transcription-PCR,multiplex PCR and real-time fluorescent PCR can be used to amplify Treponema pallidum DNA in different types of clinical specimens,including lesion swabs,blood,cerebrospinal fluid,biopsy tissue,amniotic fluid,and so on.The target genes for PCR amplification include tpp47,polA,bmp,tmpA,arp and tpr,with tpp47 and polA as the most common target genes.