CAJ | 학술논문

背景：星形胶质细胞除了被动地起代谢和结构支持的作用外，还能够以更复杂的方式与神经元相互作用，主动地参与中枢神经系统的信息处理。目的：通过观察星形胶质细胞纤维生长和突触形成的方向性和特异性，探索星形胶质细胞在与神经元相互作用中的可能机制。方法：取新生24 h内绿色荧光蛋白转基因小鼠，以原代细胞分离培养和爬片方法获得海马原代细胞，以胶质原纤维酸性蛋白荧光免疫组化方法特异性标记星形胶质细胞，在共聚焦显微镜下观察结果。结果与结论：星形胶质细胞纤维向距离较远的锥体细胞定向伸长，并且终止于锥体细胞的胞体，末端与锥体细胞的胞体形成突触样结构；而且并不是所有的突起都伸向临近的细胞，其突触样结构的形成也不全是在最临近的位点。表明星形胶质细胞纤维伸长和突触形成具有方向性和特异性，为进一步探索星形胶质细胞与锥体细胞之间的信号传导提供依据。
배경：성형효질세포제료피동지기대사화결구지지적작용외，환능구이경복잡적방식여신경원상호작용，주동지삼여중추신경계통적신식처리。목적：통과관찰성형효질세포섬유생장화돌촉형성적방향성화특이성，탐색성형효질세포재여신경원상호작용중적가능궤제。방법：취신생24 h내록색형광단백전기인소서，이원대세포분리배양화파편방법획득해마원대세포，이효질원섬유산성단백형광면역조화방법특이성표기성형효질세포，재공취초현미경하관찰결과。결과여결론：성형효질세포섬유향거리교원적추체세포정향신장，병차종지우추체세포적포체，말단여추체세포적포체형성돌촉양결구；이차병불시소유적돌기도신향림근적세포，기돌촉양결구적형성야불전시재최림근적위점。표명성형효질세포섬유신장화돌촉형성구유방향성화특이성，위진일보탐색성형효질세포여추체세포지간적신호전도제공의거。
BACKGROUND:Studies have shown that astrocytes participate not only in the passive metabolic and structural support, but also actively in the central nervous system information processing. OBJECTIVE:By observing the directionality and specificity of astrocyte fiber growth and synapse formation, to explore the possible mechanisms of astrocytes and neurons in the interaction. METHODS: Primary cels from the hippocampi of green fluorescent protein transgenic newborn mice were isolated and cultured, glial fibrilary acidic protein was used as specific marker for astrocytes in immunofluorescence staining, and then the results were observed and scanned by confocal microscope. RESULTS AND CONCLUSION:The elongation of astrocyte fibers was directional and specific, and terminated to the pyramidal cels nearby. The end of astrocyte fibers and pyramidal cels contributed to the synapse formation. However, not al the projections were toward the adjacent cels, and synapse-like structure was not only formed nearby. The results implicated that both astrocytes fiber elongation and synapse formation have the directionality and specificity, providing evidence for further studies on signal transduction between astrocytes and pyramidal cels.