CAJ | 학술논문

目的：研究红花多糖（ SPS ）对荷瘤鼠的移植瘤组织中Ang－2、PTEN蛋白表达的影响及意义。方法：选择昆明种小鼠80只制备荷瘤小鼠，随机分为4组，每组20只。对照组：模型组；实验组：环磷酰胺药物组、红花多糖＋环磷酰胺药物联合组、受试药物红花多糖组。用药方法：（1）模型对照组给荷瘤鼠灌服生理盐水；（2）环磷酰胺药物组灌服环磷酰胺；（3）红花多糖＋环磷酰胺药物联合组灌服环磷酰胺和红花多糖；（4）红花多糖组：红花多糖灌服荷瘤鼠。给药10天后停药，取肿瘤组织，称取湿重，计算抑瘤率。然后用S－P免疫组织化学法测定对照组、实验组荷瘤鼠移植瘤组织中Ang－2、PTEN蛋白的表达情况。结果：荷瘤鼠灌服生理盐水的模型对照组移植瘤组织中Ang－2的免疫组化阳性表达率显著高于各实验组（P＜0．05），而PTEN蛋白的阳性表达率显著低于各实验组（P＜0．01）。红花多糖、环磷酰胺联合用药组的抑瘤率最高，其次为环磷酰组、红花多糖组。结论：红花多糖具有抑制肿瘤组织生长的作用，可降低肿瘤组织中Ang－2的表达，从而增强PTEN蛋白的表达。
목적：연구홍화다당（ SPS ）대하류서적이식류조직중Ang－2、PTEN단백표체적영향급의의。방법：선택곤명충소서80지제비하류소서，수궤분위4조，매조20지。대조조：모형조；실험조：배린선알약물조、홍화다당＋배린선알약물연합조、수시약물홍화다당조。용약방법：（1）모형대조조급하류서관복생리염수；（2）배린선알약물조관복배린선알；（3）홍화다당＋배린선알약물연합조관복배린선알화홍화다당；（4）홍화다당조：홍화다당관복하류서。급약10천후정약，취종류조직，칭취습중，계산억류솔。연후용S－P면역조직화학법측정대조조、실험조하류서이식류조직중Ang－2、PTEN단백적표체정황。결과：하류서관복생리염수적모형대조조이식류조직중Ang－2적면역조화양성표체솔현저고우각실험조（P＜0．05），이PTEN단백적양성표체솔현저저우각실험조（P＜0．01）。홍화다당、배린선알연합용약조적억류솔최고，기차위배린선조、홍화다당조。결론：홍화다당구유억제종류조직생장적작용，가강저종류조직중Ang－2적표체，종이증강PTEN단백적표체。
Objective:To investigate the effect of Safflower Polysaccharide on the expression of Ang -2 and PTEN protein of mice transplanted with Carcinoma cells and its clinical significance .Methods:Preparation of 80 Kunming mice trans-planted with Carcinoma cells were randomly divided into 4 groups with 20 in eachh group .They were in each group a model control group,model control group: Cyclophosphamide (CTX) drug group、SPS and CTX drug group、SPS group tested drugs.Drugs for:(1)The control group:tumor-bearing mice were fed with normal saline;(2)CTX drug group:tumor-bearing mice were fed with CTX;( 3 ) SPS and CTX drug group: tumor-bearing mice were fed with SPS and CTX;(4)SPS drug group:tumor-bearing mice were fed with SPS.After tumor-bearing mice were fed for 10 days, tumor-bearing mice were killed and the tumor tissue was gained ,and then they were weighed wet weight and the inhibi-tory rate was calculated .Then,we determined expression in the model control group and the experimental group according to Ang-2 and PTEN protein with SP immunohistochemistry .Results:The Ang-2 immunohistochemical expression rate of tumor-bearing mice fed with normal saline in the group was significantly higher than the experimental groups ( P<0.05 ) ,but the PTEN protein immunohistochemical expression rate was significantly lower than the experimental groups (P<0.01).It was shown that the inhibitive rate of SPS and CTX combination therapy group was the highest , followed by CTX drug group , SPS drug group .Conclusion:SPS could inhibit the tumor growth and reduce Ang -2 expression of the tumor tissue , and could enhance PTEN protein expression of the tumor tissue .