CAJ | 학술논문

目的：建立戒酒灵中葫芦素B含量的测定方法。方法：采用超声提取戒酒灵中的葫芦素B，高效液相色谱法测定含量。色谱柱：COSMOSIL C18（250mm ×4．6mm，5μm ）；流动相：乙腈（ A）和0．2％醋酸－水溶液（B）梯度洗脱：30％～40％A（0．0～15．0min），40％～45％A（15．0～40．0min）；流速：1mL／min；检测波长：234nm；柱温：25℃。结果：葫芦素B的进样量在0．01574～0．3148mg／mL范围内线性关系良好（r＝0．9999），平均加样回收率（n＝6）为97．73％，RSD1．29％。结论：该方法可作为戒酒灵的质量控制，科学可行。
목적：건립계주령중호호소B함량적측정방법。방법：채용초성제취계주령중적호호소B，고효액상색보법측정함량。색보주：COSMOSIL C18（250mm ×4．6mm，5μm ）；류동상：을정（ A）화0．2％작산－수용액（B）제도세탈：30％～40％A（0．0～15．0min），40％～45％A（15．0～40．0min）；류속：1mL／min；검측파장：234nm；주온：25℃。결과：호호소B적진양량재0．01574～0．3148mg／mL범위내선성관계량호（r＝0．9999），평균가양회수솔（n＝6）위97．73％，RSD1．29％。결론：해방법가작위계주령적질량공제，과학가행。
Objective:To establish a method for the determination of the contents of cucurbitacin B from JJL .Methods:Ul-trasonic extraction was used to get cucurbitacin B in JJL ,with content determination by HPLC .Chromatographic column:COSMOSIL C18 (250mm ×4.6mm,5μm);mobile phase:acetonitrile (A) and 0.2% acetic acid water solution (B) gradient elution:30%~40%A (0.0~15.0min), 40%~45%A (15.0~40.0min); flow rate:1mL/min; the de-tection wavelength:234 nm;column temperature:25 ℃.Results:Cucurbitacin B in the 0.015 74~0 .314 8 mg/mL range had a good linear relationship (R=0.999 9),and the average recovery was 97.73%(n=6), RSD1.29%.Con-clusion:This method can be used as the quality control of JJL ,which is scientific and feasible .