CAJ | 학술논문

药用植物灯盏花在使用中会与飞蓬属或紫菀属植物混淆，对灯盏花 matK 基因生物信息学分析及灯盏花植物形态的研究，实现灯盏花与混淆植物的鉴定。采用 PCR 方法扩增并克隆了灯盏花 matK 基因，对该基因进行生物信息学分析，结果显示，灯盏花 matK 基因总长为1317 bp，编码438个氨基酸，无信号肽，无跨膜结构，表现为亲水性，二级结构以α螺旋、β折叠为主，亚细胞定位预测 matK 基因位于真核生物的叶绿体中，有8个蛋白结合区和3个多核苷酸结合区，用植物形态、氨基酸序列变异和系统进化树相结合，可以达到鉴别灯盏花与混淆物种作用。
약용식물등잔화재사용중회여비봉속혹자완속식물혼효，대등잔화 matK 기인생물신식학분석급등잔화식물형태적연구，실현등잔화여혼효식물적감정。채용 PCR 방법확증병극륭료등잔화 matK 기인，대해기인진행생물신식학분석，결과현시，등잔화 matK 기인총장위1317 bp，편마438개안기산，무신호태，무과막결구，표현위친수성，이급결구이α라선、β절첩위주，아세포정위예측 matK 기인위우진핵생물적협록체중，유8개단백결합구화3개다핵감산결합구，용식물형태、안기산서렬변이화계통진화수상결합，가이체도감별등잔화여혼효물충작용。
In its application,the medicinal plant Erigeron breviscapus will easily get mixed with plants of the Eeri-geron genus or Asters.This research focuses on the bioinformatic analysis of the matK gene of Erigeron breviscapus and its phytomorphology.The matK gene of Erigeron breviscapus was amplified and cloned by the PCR method be-fore its bioinformatic analysis.The results showed that the full length sequence of the matK gene of Erigeron brevis-capus was 1,317bp,and 438 encoded amino acids,with no signal peptide or transmembrane protein,and some hy-drophilic property.Its secondary structure is primarily an alpha helix and beta fold,and the subcellular localization confirmed the existence of the matK gene in the chloroplastid of eukaryotes with eight protein-binding sites and three polynucleotides.The combination of the methods of phytomorphology,the sequence variation of amino acids and the phylogenetic tree can distinguish Erigeron breviscapus from other plants.