CAJ | 학술논문

目的：探讨中药丹皮酚对人甲状腺未分化癌FRO细胞相关lncRNA表达的影响。方法：选取不同浓度的丹皮酚作用于人甲状腺未分化癌FRO 细胞，采用MTT法检测细胞的存活率，对细胞形态学的改变采用倒置相差显微镜观察，qRT－PCR检测不同浓度的中药丹皮酚作用于FRO细胞后H19、HOT、PCA三种癌症相关lncRNA的表达水平。结果：不同浓度的丹皮酚能够在不同程度上抑制甲状腺未分化癌FRO细胞的生长，经过药物处理后48h，检测发现FRO细胞中H19、HOTAIR、PCA3的表达显著增高，且随浓度增加而逐渐升高。结论：丹皮酚可以抑制甲状腺未分化癌细胞的生长，不同浓度的丹皮酚对甲状腺未分化癌细胞中的lncRNA表达有影响，说明中药提取物丹皮酚可能通过lncRNA对肿瘤的发生发展产生影响，相关机制需要进一步研究。
목적：탐토중약단피분대인갑상선미분화암FRO세포상관lncRNA표체적영향。방법：선취불동농도적단피분작용우인갑상선미분화암FRO 세포，채용MTT법검측세포적존활솔，대세포형태학적개변채용도치상차현미경관찰，qRT－PCR검측불동농도적중약단피분작용우FRO세포후H19、HOT、PCA삼충암증상관lncRNA적표체수평。결과：불동농도적단피분능구재불동정도상억제갑상선미분화암FRO세포적생장，경과약물처리후48h，검측발현FRO세포중H19、HOTAIR、PCA3적표체현저증고，차수농도증가이축점승고。결론：단피분가이억제갑상선미분화암세포적생장，불동농도적단피분대갑상선미분화암세포중적lncRNA표체유영향，설명중약제취물단피분가능통과lncRNA대종류적발생발전산생영향，상관궤제수요진일보연구。
Objective:To investigate whether the traditional Chinese medicine Paeonol can affect the expressive level of lncRNA in human thyroid cancer cell line FRO .Methods:Human thyroid cancer cell line FRO was incubated with dif-ferent concentrations of paeonol for 48 h.Then cell survival rate was measured by MTT assay and cell morphology change was observed by phase contrast microscope .The expressive level of cancer -related lncRNA (H19,HOT,PCA) of hu-man thyroid cancer cell line FRO was measured by qRT -PCR.Results:Paeonol could inhibit the growth of FRO to dif-ferent degree.After treated with Paeonol for 24 hours, the expression level of cancer -related lncRNA in FRO was in-creased .In addition , the lncRNA expression increased gradually with the increase of Paeonol concentration .Conclusion:Paeonol can inhibit the growth of FRO .Different concentrations of Paeonol have different effects on the changes of the ex -pressive levels of lncRNA in FRO .Traditional Chinese medicine Paeonol may influence the progression of human thyroid cancer by changing the expression levels of cancer -related lncRNA .Further investigation on the mechanism is needed .