CAJ | 학술논문

背景：已有研究证实羧甲基壳聚糖对许旺细胞增殖、分泌具有促进作用，但其对许旺细胞内环磷腺苷介导蛋白激酶A信号通路的影响仍需要进一步研究。目的：观察羧甲基壳聚糖对大鼠许旺细胞内环磷腺苷/蛋白激酶A信号通路的影响。方法：取第2代新生大鼠许旺细胞，以1×109 L-1的细胞浓度接种于6孔板，分4组培养，空白对照组加入PBS，实验组分别加入50，100，200 mg/L的羧甲基壳聚糖溶液培养。培养24 h后，检测许旺细胞内环磷腺苷浓度、蛋白激酶A活性及环磷腺苷反应元件结合蛋白mRNA的表达。结果与结论：与空白对照组比较，羧甲基壳聚糖呈剂量依赖性提高许旺细胞内环磷腺苷浓度(P<0.05)，呈剂量依赖性增强许旺细胞内蛋白激酶A活性(P<0.05)，呈剂量依赖性提高许旺细胞内环磷腺苷反应元件结合蛋白mRNA的表达(P<0.05)。表明羧甲基壳聚糖可增加许旺细胞内环磷腺苷浓度、促进蛋白激酶A活性，从而激活环磷腺苷/蛋白激酶A信号通路。
배경：이유연구증실최갑기각취당대허왕세포증식、분비구유촉진작용，단기대허왕세포내배린선감개도단백격매A신호통로적영향잉수요진일보연구。목적：관찰최갑기각취당대대서허왕세포내배린선감/단백격매A신호통로적영향。방법：취제2대신생대서허왕세포，이1×109 L-1적세포농도접충우6공판，분4조배양，공백대조조가입PBS，실험조분별가입50，100，200 mg/L적최갑기각취당용액배양。배양24 h후，검측허왕세포내배린선감농도、단백격매A활성급배린선감반응원건결합단백mRNA적표체。결과여결론：여공백대조조비교，최갑기각취당정제량의뢰성제고허왕세포내배린선감농도(P<0.05)，정제량의뢰성증강허왕세포내단백격매A활성(P<0.05)，정제량의뢰성제고허왕세포내배린선감반응원건결합단백mRNA적표체(P<0.05)。표명최갑기각취당가증가허왕세포내배린선감농도、촉진단백격매A활성，종이격활배린선감/단백격매A신호통로。
BACKGROUND:It has been confirmed that carboxymethylated chitosan has an promoting effect on Schwann cel proliferation and secretion, but its impact on the cyclic adenosine monophosphate-mediated protein kinase A signaling pathway in schwann cel stil needs further study. OBJECTIVE:To investigate the effect of carboxymethylated chitosan on cyclic adenosine monophosphate/ protein kinase A signaling pathway in rat schwann cels. METHODS:The Schwann cels of the second generation neonatal rats were obtained and seeded in 6-wel plate at a concentration of 1×109/L. These Schwann cels were cultured and divided into four groups. The Schwann cels in the control group were cultured by adding PBS. The Schwann cels in the experimental groups were cultured by adding 50, 100 and 200 mg/L of carboxymethyl chitosan solution, respectively. After 24 hours, the concentration of cyclic adenosine monophosphate, protein kinase A activity and cyclic adenosine monophosphate response element binding protein mRNA expression were detected. RESULTS AND CONCLUSION:Compared with the control group, carboxymethyl chitosan increased cyclic adenosine monophosphate concentrations, the activity of protein kinase A and cyclic adenosine monophosphate response element binding protein mRNA expression within the Schwann cels in a dose-dependent manner (P < 0.05). These results demonstrate that carboxymethyl chitosan can increase the concentration of cyclic adenosine monophosphate within the Schwann cels and promote protein kinase A activity, thereby activating cyclic adenosine monophosphate/protein kinase A signaling pathway.