CAJ | 학술논문

背景：目前牵张成骨由于治疗周期长、并发症较多成为临床广泛运用的瓶颈，不能满足临床推广需要。目的：以兔下颌骨牵张动物模型为实验平台，观察全身运用杜仲醇提取物对牵张新骨再生的影响。方法：24只新西兰大白兔随机均分为对照组及实验组，建立兔下颌单侧牵张模型，牵张速率为每12 h 1 mm。在牵张期2次/d，实验组及对照组分别予以灌胃杜仲醇提取物及等量生理盐水，牵张结束后6周处死动物收集标本行成骨检测。结果与结论：两组动物牵张间隙内均可观察到新骨生成。牵张成骨结束后6周下颌骨 CT图像显示实验组兔牵张间隙舌侧骨皮质生成良好，颊侧骨皮质连续，牵张间隙可见均匀骨质生成桥接；下颌骨核素扫描显示实验组兔下颌骨牵张间隙表现为核浓集，强度明显强于对照组；X 射线显示实验组牵张间隙完全桥接，新生成骨质密度均匀，可见上下侧骨皮质形成良好；Micro-CT图像显示实验组骨皮质部分形成较好，骨小梁分布较为均匀，骨小梁明显较对照组粗壮，对照组部分区域仍有囊性变；Micro-CT微结构参数检测显示实验组骨体积分数、骨小梁厚度、骨小梁数量和连接密度均显著高于对照组；组织学观察显示与对照组比较，实验组牵张间隙中有较为成熟的束状骨，骨小梁方向较为规律。实验结果显示全身运用杜仲醇提取物可有效促进兔下颌快速骨牵张的新骨再生。
배경：목전견장성골유우치료주기장、병발증교다성위림상엄범운용적병경，불능만족림상추엄수요。목적：이토하합골견장동물모형위실험평태，관찰전신운용두중순제취물대견장신골재생적영향。방법：24지신서란대백토수궤균분위대조조급실험조，건립토하합단측견장모형，견장속솔위매12 h 1 mm。재견장기2차/d，실험조급대조조분별여이관위두중순제취물급등량생리염수，견장결속후6주처사동물수집표본행성골검측。결과여결론：량조동물견장간극내균가관찰도신골생성。견장성골결속후6주하합골 CT도상현시실험조토견장간극설측골피질생성량호，협측골피질련속，견장간극가견균균골질생성교접；하합골핵소소묘현시실험조토하합골견장간극표현위핵농집，강도명현강우대조조；X 사선현시실험조견장간극완전교접，신생성골질밀도균균，가견상하측골피질형성량호；Micro-CT도상현시실험조골피질부분형성교호，골소량분포교위균균，골소량명현교대조조조장，대조조부분구역잉유낭성변；Micro-CT미결구삼수검측현시실험조골체적분수、골소량후도、골소량수량화련접밀도균현저고우대조조；조직학관찰현시여대조조비교，실험조견장간극중유교위성숙적속상골，골소량방향교위규률。실험결과현시전신운용두중순제취물가유효촉진토하합쾌속골견장적신골재생。
BACKGROUND:Long course of treatment and complications limit the extensive application of distraction osteogenesis, which cannot meet the clinical requirements. OBJECTIVE:To investigate the effect of general administration of eucommia alcohol extract on new bone regeneration based on the rabbit model of mandibular distraction osteogenesis. METHODS:Twenty-four New Zealand white rabbits were randomly divided into experimental group and control group. Unilateral mandibular distraction osteogenesis model was established by 1 mm/12 h distraction protocol. During the distraction period, the rabbits in the experimental group were intragastricaly administered with eucommia alcohol extract and the control animals received the same amount of physiological saline, respectively. Six weeks later, the animals were sacrificed for osteogenic testing. RESULTS AND CONCLUSION: New bone formation was observed in the distracted gap in both groups. However, the amount, mineralization and biomechanical strength of new regenerated bone in the experimental group were obviously greater than those in the control group by histological observation, dual energy X-ray absorptiometry, micro-CT and biomechanical test. General administration of eucommia alcohol extract can markedly promote distraction osteogensis in rabbit mandibular osteodistraction.