CAJ | 학술논문

背景：义眼戴入后结膜囊内的微生态环境被打破，其自洁作用减弱，更利于微生物的生长和定植，义眼表面菌斑生物膜的清洁效果影响佩戴舒适度和患者生活质量，寻求一种有效的清洁方法有其必要性。目的：比较5种清洗方法对义眼表面菌斑生物膜的清洁效果。方法：对84例已行义眼修复患者，取结膜囊内分泌物作细菌培养鉴定。制作自凝树脂、热凝树脂义眼片各50片，每组义眼片随机均分为5组，分别以清水、体积分数75%乙醇、Boston SIMPLUS、Polident及牙膏进行清洁，清洁完成后将试片进行残留生物膜培养，并运用菌落计数法评估不同处理方式的清洁效果。结果与结论：84例标本送检，阳性49例，其中金黄色葡萄球菌分离率14.29%，表皮葡萄球菌分离率占13.10%，麦氏棒杆菌分离率占7.14%。采用清水、Boston SIMPLUS、牙膏清洁时，自凝树脂组金黄色葡萄球菌菌落数高于热凝树脂组(P<0.05)；采用乙醇、Polident清洁时，两组金黄色葡萄球菌菌落数无差异。在自凝树脂中，清水处理组菌落计数高于其他方法处理组(P<0.05)，乙醇处理组菌落计数低于Boston SIMPLUS组(P<0.05)，其余组间比较差异均无显著性意义；在热凝树脂中，清水处理组菌落计数高于其他方法处理组(P<0.05)，其余组间比较差异均无显著性意义。表明乙醇、Boston SIMPLUS、Polident及牙膏清洗方法对两种义眼材料表面金黄色葡萄球菌生物膜的清除效果均优于清水冲洗，综合多因素，为避免义眼戴用后结膜囊内微生物感染的发生，鼓励选用Polident及Boston SIMPLUS清洁义眼片。
배경：의안대입후결막낭내적미생태배경피타파，기자길작용감약，경리우미생물적생장화정식，의안표면균반생물막적청길효과영향패대서괄도화환자생활질량，심구일충유효적청길방법유기필요성。목적：비교5충청세방법대의안표면균반생물막적청길효과。방법：대84례이행의안수복환자，취결막낭내분비물작세균배양감정。제작자응수지、열응수지의안편각50편，매조의안편수궤균분위5조，분별이청수、체적분수75%을순、Boston SIMPLUS、Polident급아고진행청길，청길완성후장시편진행잔류생물막배양，병운용균락계수법평고불동처리방식적청길효과。결과여결론：84례표본송검，양성49례，기중금황색포도구균분리솔14.29%，표피포도구균분리솔점13.10%，맥씨봉간균분리솔점7.14%。채용청수、Boston SIMPLUS、아고청길시，자응수지조금황색포도구균균락수고우열응수지조(P<0.05)；채용을순、Polident청길시，량조금황색포도구균균락수무차이。재자응수지중，청수처리조균락계수고우기타방법처리조(P<0.05)，을순처리조균락계수저우Boston SIMPLUS조(P<0.05)，기여조간비교차이균무현저성의의；재열응수지중，청수처리조균락계수고우기타방법처리조(P<0.05)，기여조간비교차이균무현저성의의。표명을순、Boston SIMPLUS、Polident급아고청세방법대량충의안재료표면금황색포도구균생물막적청제효과균우우청수충세，종합다인소，위피면의안대용후결막낭내미생물감염적발생，고려선용Polident급Boston SIMPLUS청길의안편。
BACKGROUND:The micro-ecological environment has been broken when the ocular prosthesis was inset into the conjunctival sac. The recede of self cleaning function is more conducive to the microbial growth and colonization. The cleaning of plaque biofilm on ocular prosthesis surface affects the patient's wearing comfort and quality of life. It is necessary to seek an effective cleaning method. OBJECTIVE:To compare the clearance effect of five cleaning methods on the palque biofilm on ocular prosthesis surface. METHODS: The conjunctival secretions from 84 patients who were subjected to ocular prosthesis repair were taken for bacterial culture and identification. Fifty pieces of self-curing resin and thermosetting resin artificial eyes were produced. The artificial eyes in each group were randomly divided into five groups, and were cleaned respectively with clear water, volume fraction of 75% ethanol, Boston SIMPLUS, polident and toothpaste. After the completion of the cleaning, the test piece was conducted residual biofilm culture. The clearance effects of different processing modes were evaluated using colony counting method. RESULTS AND CONCLUSION: Eighty-four specimens were submitted for inspection, of which 49 were positive. The Staphylococcusaureus separation rate was 14.29%.Staphylococcus epidermidis separation rate accounted for 13.10%. Maxwel Corynebacterium separation rate accounted for 7.14%. When water, Boston SIMPLUS and toothpaste were used for cleaning, theStaphylococcus aureus colony number in the self-curing resin group was higher than that in the thermosetting resin group (P< 0.05); when ethanol and polident were used for cleaning, there was no difference in the Staphylococcus aureus colony number between these two groups. In self-curing resin, the colony count in the clear water treatment group was higher than that in the other treatment groups (P < 0.05). The colony count in the ethanol treatment group was lower than that in the Boston SIMPLUS group (P < 0.05). There was no significant difference in the colony count between other groups. In thermosetting resin, the colony count in the clear water treatment group was higher than that in the other treatment groups (P < 0.05). There was no significant difference in the colony count between other groups. These results demonstrate that ethanol, Boston SIMPLUS, polident and toothpaste have better cleaning effects onStaphylococcus aureusbiofilms on the surface of two kinds of ocular prostheses than the clear water rinse. Overal, it is encouraged to clean the artificial eyes using polident and Boston SIMPLUS, in order to avoid the occurrence of microbial infection in the conjunctival sac after wearing ocular prosthesis.