中华妇幼临床医学杂志（电子版）
中華婦幼臨床醫學雜誌（電子版）
중화부유림상의학잡지（전자판）
Chinese Journal of Obstetrics & Gynecology and Pediatrics (Electronic Edition)
2015年
5期
564-569
,共6页
魏宝霞%安锦丹%邹丹峰%刘晓梅%王洪伟%徐晓华
魏寶霞%安錦丹%鄒丹峰%劉曉梅%王洪偉%徐曉華
위보하%안금단%추단봉%류효매%왕홍위%서효화
卵巢上皮癌%KiSS1 蛋白,人类%MTA1 蛋白,人类%免疫组织化学
卵巢上皮癌%KiSS1 蛋白,人類%MTA1 蛋白,人類%免疫組織化學
란소상피암%KiSS1 단백,인류%MTA1 단백,인류%면역조직화학
Ovarian epithelial cancer%KiSS 1 protein,human%MTA1 protein,human%Immunohistochemistry
目的：探讨肿瘤转移抑制基因 KiSS-1与肿瘤转移相关基因 MTA1在卵巢上皮性肿瘤中的表达及其临床意义。方法选择1994年1月至2003年12月在哈尔滨医科大学附属第三医院和牡丹江医学院附属红旗医院进行卵巢肿瘤切除术的121例卵巢肿瘤患者为研究对象。采用免疫组织化学 SP 法检测受试者切除卵巢肿瘤组织中 KiSS-1及 MTA1的表达,并统计学比较二者在不同病理类型卵巢上皮性肿瘤中的表达阳性率差异,在卵巢上皮性癌的不同临床病理参数下表达阳性率差异,以及在卵巢上皮性癌中表达的相关性及其表达与卵巢上皮性癌患者5年生存率之间的关系。结果①卵巢良性上皮性肿瘤及卵巢交界性上皮性肿瘤细胞质中,KiSS-1表达阳性率均显著高于卵巢上皮性癌,且差异均有统计学意义(83.3% vs 29.5%,χ2=23.375,P =0.0001；63.3% vs 29.5%,χ2=29.536,P =0.0002)；而卵巢良性上皮性肿瘤及卵巢交界性上皮性肿瘤细胞质中,MTA1表达阳性率均显著低于卵巢上皮性癌,且差异均有统计学意义(23.3% vs 73.8%,χ2=20.889,P =0.0004；40.0% vs 73.8%,χ2=9.800,P =0.0020)。② KiSS-1或 MTA1在卵巢上皮性癌的国际妇产科联盟(FIGO)临床分期、淋巴结转移及腹水等临床病理参数下的表达阳性率比较,差异均有统计学意义(P <0.05)；而在年龄、肿瘤直径、组织学类型及世界卫生组织(WHO)病理分级的临床病理参数下,表达阳性率比较,差异均无统计学意义(P >0.05)。③ KiSS-1和MTA1在卵巢上皮性癌中的表达呈负相关关系(r =-0.268,P =0.038)。④在卵巢上皮性癌中,KiSS-1表达呈阳性者的5年生存率(33.3%)显著高于 KiSS-1表达呈阴性者(14.0%),而 MTA1表达呈阳性者的5年生存率(11.1%)显著低于 MTA1表达呈阴性者(43.8%),且差异均有统计学意义(χ2=6.473,14.869；P <0.05)。结论KiSS-1与 MTA1基因可能参与了卵巢上皮性肿瘤的发生、发展和转移过程,或可作为评估恶性上皮性卵巢肿瘤预后的可行性指标。
目的：探討腫瘤轉移抑製基因 KiSS-1與腫瘤轉移相關基因 MTA1在卵巢上皮性腫瘤中的錶達及其臨床意義。方法選擇1994年1月至2003年12月在哈爾濱醫科大學附屬第三醫院和牡丹江醫學院附屬紅旂醫院進行卵巢腫瘤切除術的121例卵巢腫瘤患者為研究對象。採用免疫組織化學 SP 法檢測受試者切除卵巢腫瘤組織中 KiSS-1及 MTA1的錶達,併統計學比較二者在不同病理類型卵巢上皮性腫瘤中的錶達暘性率差異,在卵巢上皮性癌的不同臨床病理參數下錶達暘性率差異,以及在卵巢上皮性癌中錶達的相關性及其錶達與卵巢上皮性癌患者5年生存率之間的關繫。結果①卵巢良性上皮性腫瘤及卵巢交界性上皮性腫瘤細胞質中,KiSS-1錶達暘性率均顯著高于卵巢上皮性癌,且差異均有統計學意義(83.3% vs 29.5%,χ2=23.375,P =0.0001；63.3% vs 29.5%,χ2=29.536,P =0.0002)；而卵巢良性上皮性腫瘤及卵巢交界性上皮性腫瘤細胞質中,MTA1錶達暘性率均顯著低于卵巢上皮性癌,且差異均有統計學意義(23.3% vs 73.8%,χ2=20.889,P =0.0004；40.0% vs 73.8%,χ2=9.800,P =0.0020)。② KiSS-1或 MTA1在卵巢上皮性癌的國際婦產科聯盟(FIGO)臨床分期、淋巴結轉移及腹水等臨床病理參數下的錶達暘性率比較,差異均有統計學意義(P <0.05)；而在年齡、腫瘤直徑、組織學類型及世界衛生組織(WHO)病理分級的臨床病理參數下,錶達暘性率比較,差異均無統計學意義(P >0.05)。③ KiSS-1和MTA1在卵巢上皮性癌中的錶達呈負相關關繫(r =-0.268,P =0.038)。④在卵巢上皮性癌中,KiSS-1錶達呈暘性者的5年生存率(33.3%)顯著高于 KiSS-1錶達呈陰性者(14.0%),而 MTA1錶達呈暘性者的5年生存率(11.1%)顯著低于 MTA1錶達呈陰性者(43.8%),且差異均有統計學意義(χ2=6.473,14.869；P <0.05)。結論KiSS-1與 MTA1基因可能參與瞭卵巢上皮性腫瘤的髮生、髮展和轉移過程,或可作為評估噁性上皮性卵巢腫瘤預後的可行性指標。
목적：탐토종류전이억제기인 KiSS-1여종류전이상관기인 MTA1재란소상피성종류중적표체급기림상의의。방법선택1994년1월지2003년12월재합이빈의과대학부속제삼의원화모단강의학원부속홍기의원진행란소종류절제술적121례란소종류환자위연구대상。채용면역조직화학 SP 법검측수시자절제란소종류조직중 KiSS-1급 MTA1적표체,병통계학비교이자재불동병리류형란소상피성종류중적표체양성솔차이,재란소상피성암적불동림상병리삼수하표체양성솔차이,이급재란소상피성암중표체적상관성급기표체여란소상피성암환자5년생존솔지간적관계。결과①란소량성상피성종류급란소교계성상피성종류세포질중,KiSS-1표체양성솔균현저고우란소상피성암,차차이균유통계학의의(83.3% vs 29.5%,χ2=23.375,P =0.0001；63.3% vs 29.5%,χ2=29.536,P =0.0002)；이란소량성상피성종류급란소교계성상피성종류세포질중,MTA1표체양성솔균현저저우란소상피성암,차차이균유통계학의의(23.3% vs 73.8%,χ2=20.889,P =0.0004；40.0% vs 73.8%,χ2=9.800,P =0.0020)。② KiSS-1혹 MTA1재란소상피성암적국제부산과련맹(FIGO)림상분기、림파결전이급복수등림상병리삼수하적표체양성솔비교,차이균유통계학의의(P <0.05)；이재년령、종류직경、조직학류형급세계위생조직(WHO)병리분급적림상병리삼수하,표체양성솔비교,차이균무통계학의의(P >0.05)。③ KiSS-1화MTA1재란소상피성암중적표체정부상관관계(r =-0.268,P =0.038)。④재란소상피성암중,KiSS-1표체정양성자적5년생존솔(33.3%)현저고우 KiSS-1표체정음성자(14.0%),이 MTA1표체정양성자적5년생존솔(11.1%)현저저우 MTA1표체정음성자(43.8%),차차이균유통계학의의(χ2=6.473,14.869；P <0.05)。결론KiSS-1여 MTA1기인가능삼여료란소상피성종류적발생、발전화전이과정,혹가작위평고악성상피성란소종류예후적가행성지표。
Objective To investigate the expression and clinical significance of tumor metastasis suppressor gene KiSS-1 (KiSS-1 )and metastasis associated gene 1 (MTA1 )in epithelial ovarian tumor. Methods A total of 121 ovarian tumor patients who were underwent ovarian tumor resection in the Third Hospital Affiliated to Harbin Medical University and Hongqi Hospital Affiliated to Mudanjiang Medical School from January 1 994 to December 2003 were chosen as research objects.Histochemical technique was used to detect the KiSS-1 and MTAl protein expression levels in 121 ovarian tumor samples.Statistical method was used to compare the differences of expression positive rates of KiSS-1 and MTA1 in different pathological types of epithelial ovarian tumors and in different clinical pathological parameters of ovarian epithelial carcinoma,also the correlation of expression levels of these two proteins in ovarian epithelial carcinoma and the relationship between their expression level and five year survival rate of ovarian epithelial carcinoma.Results ① Expression positive rates of KiSS-1 in benign ovarian epithelial and borderline of epithelial ovarian tumors in cytoplasm were both higher than that of in ovarian epithelial carcinoma,and the differences were statistically significant(83.3% vs 29.5%,χ2 =23.375,P =0.000 1;63.3% vs 29.5%,χ2 =29.536,P = 0.000 2 );However,expression positive rates of MTA1 in benign ovarian epithelial and borderline of epithelial ovarian tumors in cytoplasm were both lower than that of in ovarian epithelial carcinoma,and the differences were statistically significant(23.3% vs 73.8%,χ2 = 20.889,P = 0.000 4;40.0% vs 73.8%,χ2 = 9.800,P = 0.002 0 ).② There were significant differences of KiSS-1 or MAT1 expression positive rate in clinical pathological parameters of International Federation of Gynecology and Obstetrics(FIGO)clinical stage,lymph node metastasis and ascites(P <0.05),but there were no significant differences in clinical pathological parameters of age,tumor size,histological types and the WHO pathological grades(P >0.05).③ The expression of KiSS-1 and MTA1 in ovarian epithelial carcinoma were negatively correlation(r = - 0.268,P = 0.038 ).④ In ovarian epithelial carcinoma,5 year survival rate of KiSS-1 positive patients(33.3%)was higher than that of KiSS-1 negative ones(14.0%),While,5 year survival rate of MTA1 positive patients (1 1.1%)was lower than that of MAT1 negative ones (43.8%),and the differences were statistically significant(χ2 = 6.473,14.869;P < 0.05 ).Conclusions The expressions of KiSS-1 and MTA1 gene may participate in carcinogenesis,invasion and metastasis of epithelial ovarian tumor,which may contribute to prognosis marker of malignant epithelial ovarian tumor.
