CAJ | 학술논문

目的：探讨 SD 大鼠孕期宫内慢性缺氧(CIH)后,其子代大鼠(简称为子鼠)内耳 Cx26表达水平及内耳发育改变。方法选择12只健康 SD 孕大鼠为研究对象,并随机分为 CIH 组及正常组,每组各6只。对 CIH 组建立 CIH 孕鼠模型。统计学比较两组子鼠生后不同日龄的体质量差异,并在两组子鼠日龄为56 d 时获取其内耳标本,分别采用光镜(HE 染色)及电镜观察子鼠内耳毛细胞发育改变,采用原位细胞凋亡检测(TUNEL)法检测内耳考蒂器毛细胞凋亡情况。蛋白质印迹法比较子鼠内耳 Cx26表达水平改变。结果① CIH 组新生子鼠生后12 h 内,体质量显著低于正常组子鼠,且差异有统计学意义[(4.8±0.6)g vs (6.0±0.4)g,t=4.076,P =0.002]。②光镜及电镜检查结果显示,CIH 组考蒂器毛细胞缺失,电镜下可见毛细胞凋亡现象；CIH 组子鼠耳蜗考蒂器毛细胞、血管纹细胞及螺旋神经节平均相对光密度值,均较正常组显著增高,且差异均有统计学意义[(0.42±0.11)vs (0.26±0.09),t =2.758,P =0.0200；(0.40±0.14)vs (0.19±0.05),t =3.460,P =0.0060；(0.45±0.12)vs (0.19±0.03),t =5.149,P =0.0004]。③ CIH 组子鼠内耳 Cx26蛋白表达水平较正常组显著降低,且差异有统计学意义[(0.72±0.36)mg/(kg·d)vs (1.07±0.19)mg/(kg·d),t=0.785,P =0.042]。结论SD 大鼠产前发生 CIH,可导致胎鼠宫内生长受限、子鼠低出生体质量及听力损伤。
목적：탐토 SD 대서잉기궁내만성결양(CIH)후,기자대대서(간칭위자서)내이 Cx26표체수평급내이발육개변。방법선택12지건강 SD 잉대서위연구대상,병수궤분위 CIH 조급정상조,매조각6지。대 CIH 조건립 CIH 잉서모형。통계학비교량조자서생후불동일령적체질량차이,병재량조자서일령위56 d 시획취기내이표본,분별채용광경(HE 염색)급전경관찰자서내이모세포발육개변,채용원위세포조망검측(TUNEL)법검측내이고체기모세포조망정황。단백질인적법비교자서내이 Cx26표체수평개변。결과① CIH 조신생자서생후12 h 내,체질량현저저우정상조자서,차차이유통계학의의[(4.8±0.6)g vs (6.0±0.4)g,t=4.076,P =0.002]。②광경급전경검사결과현시,CIH 조고체기모세포결실,전경하가견모세포조망현상；CIH 조자서이와고체기모세포、혈관문세포급라선신경절평균상대광밀도치,균교정상조현저증고,차차이균유통계학의의[(0.42±0.11)vs (0.26±0.09),t =2.758,P =0.0200；(0.40±0.14)vs (0.19±0.05),t =3.460,P =0.0060；(0.45±0.12)vs (0.19±0.03),t =5.149,P =0.0004]。③ CIH 조자서내이 Cx26단백표체수평교정상조현저강저,차차이유통계학의의[(0.72±0.36)mg/(kg·d)vs (1.07±0.19)mg/(kg·d),t=0.785,P =0.042]。결론SD 대서산전발생 CIH,가도치태서궁내생장수한、자서저출생체질량급은력손상。
Objective To study the changes of connexin-26 (Cx26 )expression level and inner ear development of offspring rats after SD pregnant rats suffering from chronic intrauterine hypoxia(CIH)in gestation period.Methods A total of 12 healthy SD pregnant rats were chosen as research objects and randomly divided into CIH group and normal group,each consisted 6 SD pregnant rats.CIH pregnant rat models were established for the CIH group.Compared the postnatal weight of different days of offspring rats between two groups statistically and inner ear specimen of offspring rats were extracted on the 56th day after born.Light microscope (HE staining)and electron microscope were used to observe the changes of hair cell development in inner ears.In Situ Nick-End Labeling(TUNEL)method was used to detect the apoptosis of hair cells in corti organ.Western blotting method was used to detect the changes of Cx26 protein expression level in inner ears.Results ① The weight of newborn offspring rats within 12 hours after birth in CIH group was lower than that of normal group,and the difference was statistically significant [(4.8±0.6)g vs (6.0±0.4)g,t =4.076,P =0.002].② The results of light and electron microscopic examination showed that the hair cells in corti organ were deficient and apoptosis could be seen under the electron microscope in CIH group.The relative value of mean optical density of corti organ hair cells,stria vascularis cells and spiral ganglion in cochlear of CIH group were higher than those of normal group,and the differences were statistically significant[(0.42±0.1 1)vs (0.26 ±0.09),t =2.758,P =0.020 0;(0.40 ±0.14)vs (0.1 9 ± 0.05),t=3.460,P =0.006 0;(0.45±0.12)vs (0.1 9±0.03),t=5.149,P =0.000 4].③ The expression level of Cx26 protein of offspring rats of CIH group were lower than that of normal group,and the difference was statistically significant[(0.72 ± 0.36 )mg/(kg · d)vs (1.07 ± 0.1 9 )mg/(kg · d),t = 0.785,P =0.042].Conclusions Prenatal CIH of SD rats results in intrauterine growth restriction of fetal rats, therefore,offspring rats are born with low birth weight and suffer from hearing damage.