CAJ | 학술논문

背景：设计、制备新型温敏高分子三维细胞支架是目前高分子科学研究的热点之一。目的：制备3种不同的温控脱细胞载体，并评估其性能。方法：分别制备共聚异丙基丙烯酰胺温控脱细胞支架、大孔共聚异丙基丙烯酰胺温控脱细胞支架与大孔共聚异丙基丙烯酰胺交联醛基化海藻酸钠温控脱细胞支架，检测3组支架的比表面积、温敏性能、孔隙率、孔径及细胞相容性。结果与结论：共聚异丙基丙烯酰胺温控脱细胞支架、大孔共聚异丙基丙烯酰胺温控脱细胞支架与大孔共聚异丙基丙烯酰胺交联醛基化海藻酸钠温控脱细胞支架的比表面积分别为135，386，421 m2/g，最低临界溶解温度分别为30，28.5，29.5℃，细胞毒性反应分别为2级、2级、1级，说明3种支架均具备温度敏感特性，而且最低临界溶解温度相近；大孔共聚异丙基丙烯酰胺交联醛基化海藻酸钠温控脱细胞支架的细胞相容性明显优于另两种支架。大孔共聚异丙基丙烯酰胺温控脱细胞支架、大孔共聚异丙基丙烯酰胺交联醛基化海藻酸钠温控脱细胞支架的孔隙率与孔径大于共聚异丙基丙烯酰胺温控脱细胞支架(P <0.05)，说明大孔共聚异丙基丙烯酰胺温控脱细胞支架与大孔共聚异丙基丙烯酰胺交联醛基化海藻酸钠温控脱细胞支架具有更加明显的孔洞结构。
배경：설계、제비신형온민고분자삼유세포지가시목전고분자과학연구적열점지일。목적：제비3충불동적온공탈세포재체，병평고기성능。방법：분별제비공취이병기병희선알온공탈세포지가、대공공취이병기병희선알온공탈세포지가여대공공취이병기병희선알교련철기화해조산납온공탈세포지가，검측3조지가적비표면적、온민성능、공극솔、공경급세포상용성。결과여결론：공취이병기병희선알온공탈세포지가、대공공취이병기병희선알온공탈세포지가여대공공취이병기병희선알교련철기화해조산납온공탈세포지가적비표면적분별위135，386，421 m2/g，최저림계용해온도분별위30，28.5，29.5℃，세포독성반응분별위2급、2급、1급，설명3충지가균구비온도민감특성，이차최저림계용해온도상근；대공공취이병기병희선알교련철기화해조산납온공탈세포지가적세포상용성명현우우령량충지가。대공공취이병기병희선알온공탈세포지가、대공공취이병기병희선알교련철기화해조산납온공탈세포지가적공극솔여공경대우공취이병기병희선알온공탈세포지가(P <0.05)，설명대공공취이병기병희선알온공탈세포지가여대공공취이병기병희선알교련철기화해조산납온공탈세포지가구유경가명현적공동결구。
BACKGROUND:To design and fabricate a novel three-dimensional thermoresponsive polymer cel scaffold is one of the hot topics in the research of polymer science. OBJECTIVE: To prepare three different kinds of thermoresponsive acelular carriers and to evaluate their performance. METHODS:The copolymer N-isopropylacrylamide temperature acelular scaffold, macroporous copolymer N-isopropylacrylamide temperature acelular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acelular scaffold were prepared. The specific surface area, thermoresponsive performance, porosity, pore size and biocompatibility of these three groups of scaffolds were determined. RESULTS AND CONCLUSION: The specific surface area of copolymer N-isopropylacrylamide thermoresponsive acelular scaffold, macroporous copolymer N-isopropylacrylamide thermoresponsive acelular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive celular scaffold was respectively 135, 386, 421 m2/g. The lower critical solution temperature was 30, 28.5, 29.5℃. The cel toxicity reaction was respectively grade 2, 2, 1. These indicators showed that the three kinds of scaffolds were provided with a temperature-sensitive characteristics and similar lower critical solution temperature. The biocompatibility of macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acelular scaffold was significantly better than the other two scaffolds. The porosity and pore size of macroporous copolymer N-isopropylacrylamide thermoresponsive acelular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acelular scaffold were greater than those of the copolymer N-isopropylacrylamide thermoresponsive acelular scaffold (P < 0.05). These results demonstrate that macroporous copolymer N-isopropylacrylamide thermoresponsive acelular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acelular scaffold have more obvious pore structure.