CAJ | 학술논문

背景：止血敷料是作用于创伤表面与人体组织直接接触，其生物相容性是评价敷料优劣的重要指标之一，以海藻酸钙为原料的制备的止血敷料有着廉价、相容性好等优点已成为研究的热点。目的：观察海藻酸钙止血敷料的细胞毒性，并与明胶止血海绵、纳吸棉、普通纱布等材料作对比。方法：①浸提液法：以DMEM高糖培养液作为浸提介质，分别制备海藻酸钙止血敷料、明胶止血海绵、纳吸棉、普通纱布浸提液，并设置100%，75%，50%，25%，10%5个浓度梯度；采用上述材料浸提液培养L-929小鼠成纤维细胞24 h，以含体积分数10%DMEM高糖培养液为空白对照，以含5%二甲基亚砜DMEM高糖培养液为阳性对照组，观察细胞增殖及形态变化。②直接接触法：将 L-929小鼠成纤维细胞分别接种于海藻酸钙止血敷料、明胶止血海绵、纳吸棉、普通纱布上培养24 h，观察细胞形态变化。结果与结论：①浸提液法：不同浓度梯度海藻纤维止血敷料、纱布、纳吸棉浸提液的细胞毒性均为1级，符合GB/T16886/ISO10993医疗器械生物学评价标准；100%，75%明胶止血海绵浸提液的细胞毒性为3级，严重抑制细胞增殖。②直接接触法：纱布与海藻纤维止血敷料的细胞毒性1级，纳吸棉为2级，明胶止血海绵的细胞毒性为3级。表明海藻酸钙止血敷料无细胞毒性。
배경：지혈부료시작용우창상표면여인체조직직접접촉，기생물상용성시평개부료우렬적중요지표지일，이해조산개위원료적제비적지혈부료유착렴개、상용성호등우점이성위연구적열점。목적：관찰해조산개지혈부료적세포독성，병여명효지혈해면、납흡면、보통사포등재료작대비。방법：①침제액법：이DMEM고당배양액작위침제개질，분별제비해조산개지혈부료、명효지혈해면、납흡면、보통사포침제액，병설치100%，75%，50%，25%，10%5개농도제도；채용상술재료침제액배양L-929소서성섬유세포24 h，이함체적분수10%DMEM고당배양액위공백대조，이함5%이갑기아풍DMEM고당배양액위양성대조조，관찰세포증식급형태변화。②직접접촉법：장 L-929소서성섬유세포분별접충우해조산개지혈부료、명효지혈해면、납흡면、보통사포상배양24 h，관찰세포형태변화。결과여결론：①침제액법：불동농도제도해조섬유지혈부료、사포、납흡면침제액적세포독성균위1급，부합GB/T16886/ISO10993의료기계생물학평개표준；100%，75%명효지혈해면침제액적세포독성위3급，엄중억제세포증식。②직접접촉법：사포여해조섬유지혈부료적세포독성1급，납흡면위2급，명효지혈해면적세포독성위3급。표명해조산개지혈부료무세포독성。
BACKGROUND:Hemostatic dressing can directly contact with the body tissues on the wound surface. The biocompatibility is one of the important indicators of evaluating the advantages and disadvantages of dressing. The hemostatic dressing prepared with calcium alginate as raw material has become a research focus owing to its low cost and good compatibility. OBJECTIVE:To observe the cytotoxicity of calcium alginate hemostatic dressings and to compare the cytotoxicity between calcium alginate hemostatic dressing and gelatin hemostatic sponge, absorbing cotton, ordinary gauze. METHEDS:Leaching solution method: the DMEM high glucose culture solution was taken as the leaching medium. The calcium alginate hemostatic dressing, gelatin hemostatic sponge, absorbing cotton and ordinary gauze leaching solution were respectively prepared. Five concentration gradients of 100%, 75%, 50%, 25%, 50% were set. The fibroblast cels of L-929 mouse were cultured for 24 hours with the above material leaching solution. The volume fraction of 10% DMEM high glucose culture medium was taken as control group, and DMEM high glucose culture medium containing 5% DMSO was taken as positive control group to observe the cel proliferation and morphological changes. Direct contact method: The fibroblast cels of L-929 mouse were respectively seeded in calcium alginate hemostatic dressing, gelatin hemostatic sponge, absorbing cotton and ordinary gauze and cultured for 24 hours. The changes in cel morphology were observed. <br> RESULTS AND CONCLUSION: Leaching solution method: The cytotoxicity of alginate fiber hemostatic dressing, gauze, absorbing cotton leaching solution with different concentration gradients was grade 1, which was in line with GB/T16886/ ISO10993 biological evaluation standard of medical apparatus and instruments. The cytotoxicity of 100%, 75% gelatin hemostatic sponge extract solution was grade 3, causing severe inhibition of cel proliferation. Direct contact method: The cytotoxicity of gauze and alginate fiber hemostatic dressing was grade 1, absorbing cotton was grade 2, gelatin hemostatic sponge was grade 3. These results demonstrate that calcium alginate hemostatic dressing has no cytotoxicity.