CAJ | 학술논문

背景：虽然镍钛合金封堵器置入治疗先天性心脏病具有较好的临床效果，但后期发生心律失常并发症的概率较大。目的：观察镍钛合金对大鼠心肌细胞肌浆网钙泵及雷尼丁受体表达的影响。方法：取30只SD大鼠，随机均分为2组，实验组于心尖部植入镍钛合金丝，对照组不做任何处理特殊处理，术后1，3，6个月取材，采用RT-PCR检测心肌细胞肌浆网钙泵与雷尼丁受体基因的表达，采用免疫组化检测心肌细胞肌浆网钙泵及雷尼丁受体蛋白的表达，采用苏木精-伊红染色检测炎症反应情况。结果与结论：实验组镍钛合金植入大鼠心肌后，炎症细胞浸润，引发炎症反应，伴纤维组织增生，随着植入时间延长，炎症反应逐渐消失；对照组未见炎细胞浸润。两组术后不同时间点的心肌细胞肌浆网钙泵、雷尼丁受体基因表达比较差异无显著性意义，相应的蛋白表达比较差异也无显著性意义。表明镍钛合金丝不影响心肌细胞肌浆网钙泵、雷尼丁受体的表达，提示镍钛合金封堵器相关心律失常可能与肌浆网钙泵、雷尼丁受体蛋白表达异常机制关系不大。
배경：수연얼태합금봉도기치입치료선천성심장병구유교호적림상효과，단후기발생심률실상병발증적개솔교대。목적：관찰얼태합금대대서심기세포기장망개빙급뢰니정수체표체적영향。방법：취30지SD대서，수궤균분위2조，실험조우심첨부식입얼태합금사，대조조불주임하처리특수처리，술후1，3，6개월취재，채용RT-PCR검측심기세포기장망개빙여뢰니정수체기인적표체，채용면역조화검측심기세포기장망개빙급뢰니정수체단백적표체，채용소목정-이홍염색검측염증반응정황。결과여결론：실험조얼태합금식입대서심기후，염증세포침윤，인발염증반응，반섬유조직증생，수착식입시간연장，염증반응축점소실；대조조미견염세포침윤。량조술후불동시간점적심기세포기장망개빙、뢰니정수체기인표체비교차이무현저성의의，상응적단백표체비교차이야무현저성의의。표명얼태합금사불영향심기세포기장망개빙、뢰니정수체적표체，제시얼태합금봉도기상관심률실상가능여기장망개빙、뢰니정수체단백표체이상궤제관계불대。
BACKGROUND:Although the nickel-titanium occluder in the treatment of congenital heart disease has a better clinical effect, arrhythmia wil be more likely to develop in late stage. OBJECTIVE:To investigate the effect of nickel-titanium wire on expression of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor of rat myocardial cels. METHODS:Thirty Sprague-Dawley rats were obtained and randomly divided into two groups: experimental and control groups. The nickel-titanium wire was implanted to the apex of heart of rats in the experimental group. Rats in the control group received no special treatment. Rat mycardial cels were harvested at the 1th, 3rdand 6th months after operation. The gene and protein expressions of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor of rat myocardial cels were detected using RT-PCR and immunohistochemistry, respectively. The inflammatory reactions were detected using hematoxylin-eosin staining. RESULTS AND CONCLUSION:After the nickel-titanium wire was implanted into the rat myocardium, inflammatory reaction was induced by inflammatory cel infiltration in the experimental group, with hyperplasia of fibrous tissue. The inflammatory reaction gradualy disappeared as the implanted time extended. No inflammatory cel infiltration was visible in the control group. There was no significant difference in the gene and protein expressions of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor of rat myocardial cels at different time points after operation between these two groups. It showed that nickel-titanium wire had no influence on the expressions of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor of rat myocardial cels. These results suggest that nickel-titanium occluder-related arrhythmia may have little relationship with abnormal protein expression of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor.