CAJ | 학술논문

背景：前期研究发现人血小板源性生长因子B基因转染的牙龈成纤维细胞能够在体外快速增殖，且能够向胞外分泌血小板源性生长因子BB蛋白。目的：了解人血小板源性生长因子B基因修饰牙龈成纤维细胞植入脱细胞真皮基质后，在体内形成牙周组织工程化复合物的能力。方法：将人血小板源性生长因子B基因转染与未转染的Beagle犬牙龈成纤维细胞分别接种于脱细胞真皮基质上，观察细胞在脱细胞真皮基质上的生长情况。将人血小板源性生长因子B基因转染犬牙龈成纤维细胞-脱细胞真皮基质复合物(实验组)、犬牙龈成纤维细胞-脱细胞真皮基质复合物(对照组)及脱细胞真皮基质(空白组)分别植入裸鼠背部皮下，植入后2，4，8周，取背部标本进行组织学观察。结果与结论：人血小板源性生长因子B基因转染与未转染的犬牙龈成纤维细胞均能在脱细胞真皮基质上良好生长。植入后8周，空白组周围的细胞大面积进入脱细胞真皮基质内，部分脱细胞真皮基质出现完全自体化，尽管细胞进入较多，但新生成的胶原纤维较少，细胞只是占据原有的胶原支架生长；对照组开始出现大面积新生胶原纤维，脱细胞真皮基质上原有的胶原纤维逐步被新生的胶原替代，但原有的胶原结构得到保留；实验组出现大面积完全矿化，可见沿原有胶原支架排列的矿化颗粒。表明接种人血小板源性生长因子B基因修饰牙龈成纤维细胞的脱细胞真皮基质在体内获得了成骨性能。
배경：전기연구발현인혈소판원성생장인자B기인전염적아간성섬유세포능구재체외쾌속증식，차능구향포외분비혈소판원성생장인자BB단백。목적：료해인혈소판원성생장인자B기인수식아간성섬유세포식입탈세포진피기질후，재체내형성아주조직공정화복합물적능력。방법：장인혈소판원성생장인자B기인전염여미전염적Beagle견아간성섬유세포분별접충우탈세포진피기질상，관찰세포재탈세포진피기질상적생장정황。장인혈소판원성생장인자B기인전염견아간성섬유세포-탈세포진피기질복합물(실험조)、견아간성섬유세포-탈세포진피기질복합물(대조조)급탈세포진피기질(공백조)분별식입라서배부피하，식입후2，4，8주，취배부표본진행조직학관찰。결과여결론：인혈소판원성생장인자B기인전염여미전염적견아간성섬유세포균능재탈세포진피기질상량호생장。식입후8주，공백조주위적세포대면적진입탈세포진피기질내，부분탈세포진피기질출현완전자체화，진관세포진입교다，단신생성적효원섬유교소，세포지시점거원유적효원지가생장；대조조개시출현대면적신생효원섬유，탈세포진피기질상원유적효원섬유축보피신생적효원체대，단원유적효원결구득도보류；실험조출현대면적완전광화，가견연원유효원지가배렬적광화과립。표명접충인혈소판원성생장인자B기인수식아간성섬유세포적탈세포진피기질재체내획득료성골성능。
BACKGROUND:Previous studies have found that human platelet-derived growth factor-B (PDGF-B)-transfected gingival fibroblasts are capable of rapid proliferationin vitro, which can secrete platelet-derived growth factor BB proteins. OBJECTIVE:To explore the ability of PDGF-B-modified gingival fibroblasts in the acelular dermal matrixin vivo to form periodontal tissue engineering compound. METHODS: Gingival fibroblasts from Beagle dogs transfected with or without PDGF-B gene were implanted into the acelular dermal matrix. Cel growth on the acelular dermal matrix was observed. PDGF-B gene-transfected gingival fibroblasts/acelular dermal matrix composite (experimental group), gingival fibrobalsts/acelular dermal matrix composite (control group) and acelular dermal matrix (blank group) were implanted subcutaneously into the nude mice, respectively. At 2, 4, 8 weeks after implantation, skin tissues were taken and observed histologicaly. RESULTS AND CONCLUSION: PDGF-B gene-modified gingival fibroblasts and non-transfected gingival fibroblasts both grew and proliferated wel in the acelular dermal matrix. At 8 weeks after implantation, in the blank group, the surrounding cels largely entered into the acelular dermal matrix, but produce less new colagen fibers, and the cels only grew on the original colagen scaffold; in the control group, a great amount of colagen fibers formed, the original colagen fibers in the acelular dermal matrix were replaced by newly formed colagens, but the original colagen structure was reserved; in the experimental group, a large scale of permineralization formed, and mineralized nodes were arranged along the original colagen scaffold. These findings indicate that PDGF-B gene modified gingival fibroblasts can acquire osteoplastic abilities in the acelular dermal matrix in vivo.