中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2015年
11期
809-811
,共3页
孙昂远%刘越阳%李铁男%孙晓冬%孙晓杰%陈晴燕%王强
孫昂遠%劉越暘%李鐵男%孫曉鼕%孫曉傑%陳晴燕%王彊
손앙원%류월양%리철남%손효동%손효걸%진청연%왕강
银屑病%肿瘤坏死因子α%白细胞介素17%白细胞介素类%掌跖脓疱病%白细胞介素22%白细胞介素17F
銀屑病%腫瘤壞死因子α%白細胞介素17%白細胞介素類%掌蹠膿皰病%白細胞介素22%白細胞介素17F
은설병%종류배사인자α%백세포개소17%백세포개소류%장척농포병%백세포개소22%백세포개소17F
Psoriasis%Tumor necrosis factor-alpha%Interleukin-17%Intedeukins%Pustulosis palmaris et plantaris%Interleukin-22%Interleukin-17F
目的 了解掌跖脓疱病患者血清肿瘤坏死因子α(TNF-α)、白细胞介素(IL)17、IL-22和IL-17F表达水平,探讨它们和疾病活动的相关性.方法 掌跖脓疱病患者30例和健康人对照20例.采取掌跖脓疱病患者活动期、静止期及对照组外周静脉血,采用ELISA法检测外周血中TNF-α、IL-17、IL-22及IL-17F含量.患者组活动期与静止期细胞因子水平的差异采用配对秩和检验比较,组间差异采用Mann-Whitney U检验比较.结果 掌跖脓疱病患者活动期外周血TNF-α、IL-17、IL-22表达水平[中位数(范围)为186.35(113.48 ~ 412.69) ng/L、420.45(278.55 ~ 748.73) ng/L、106.48(69.13 ~ 251.86) ng/L)]分别高于静止期[42.52(18.83 ~ 95.37) ng/L、48.11(36.43~80.04) ng/L、20.32(10.55 ~48.75) ng/L),T值分别为1.5、2.0、3.0,均P< 0.05]和对照组[24.30(12.09 ~ 61.56) ng/L、10.49(6.24~24.44) ng/L、2.58(1.41~5.78) ng/L,u值分别为4.17、3.84、4.69,均P<0.05];静止期也显著高于对照组,差异有统计学意义(u值分别为2.71、3.53、2.18,均P<0.05).但各组间IL-17F差异无统计学意义(均P>0.05).结论 掌跖脓疱病患者外周血TNF-α、IL-17、IL-22表达水平与疾病活动相关,提示TNF-α、IL-17和IL-22可能参与掌跖脓疱病的发病过程.
目的 瞭解掌蹠膿皰病患者血清腫瘤壞死因子α(TNF-α)、白細胞介素(IL)17、IL-22和IL-17F錶達水平,探討它們和疾病活動的相關性.方法 掌蹠膿皰病患者30例和健康人對照20例.採取掌蹠膿皰病患者活動期、靜止期及對照組外週靜脈血,採用ELISA法檢測外週血中TNF-α、IL-17、IL-22及IL-17F含量.患者組活動期與靜止期細胞因子水平的差異採用配對秩和檢驗比較,組間差異採用Mann-Whitney U檢驗比較.結果 掌蹠膿皰病患者活動期外週血TNF-α、IL-17、IL-22錶達水平[中位數(範圍)為186.35(113.48 ~ 412.69) ng/L、420.45(278.55 ~ 748.73) ng/L、106.48(69.13 ~ 251.86) ng/L)]分彆高于靜止期[42.52(18.83 ~ 95.37) ng/L、48.11(36.43~80.04) ng/L、20.32(10.55 ~48.75) ng/L),T值分彆為1.5、2.0、3.0,均P< 0.05]和對照組[24.30(12.09 ~ 61.56) ng/L、10.49(6.24~24.44) ng/L、2.58(1.41~5.78) ng/L,u值分彆為4.17、3.84、4.69,均P<0.05];靜止期也顯著高于對照組,差異有統計學意義(u值分彆為2.71、3.53、2.18,均P<0.05).但各組間IL-17F差異無統計學意義(均P>0.05).結論 掌蹠膿皰病患者外週血TNF-α、IL-17、IL-22錶達水平與疾病活動相關,提示TNF-α、IL-17和IL-22可能參與掌蹠膿皰病的髮病過程.
목적 료해장척농포병환자혈청종류배사인자α(TNF-α)、백세포개소(IL)17、IL-22화IL-17F표체수평,탐토타문화질병활동적상관성.방법 장척농포병환자30례화건강인대조20례.채취장척농포병환자활동기、정지기급대조조외주정맥혈,채용ELISA법검측외주혈중TNF-α、IL-17、IL-22급IL-17F함량.환자조활동기여정지기세포인자수평적차이채용배대질화검험비교,조간차이채용Mann-Whitney U검험비교.결과 장척농포병환자활동기외주혈TNF-α、IL-17、IL-22표체수평[중위수(범위)위186.35(113.48 ~ 412.69) ng/L、420.45(278.55 ~ 748.73) ng/L、106.48(69.13 ~ 251.86) ng/L)]분별고우정지기[42.52(18.83 ~ 95.37) ng/L、48.11(36.43~80.04) ng/L、20.32(10.55 ~48.75) ng/L),T치분별위1.5、2.0、3.0,균P< 0.05]화대조조[24.30(12.09 ~ 61.56) ng/L、10.49(6.24~24.44) ng/L、2.58(1.41~5.78) ng/L,u치분별위4.17、3.84、4.69,균P<0.05];정지기야현저고우대조조,차이유통계학의의(u치분별위2.71、3.53、2.18,균P<0.05).단각조간IL-17F차이무통계학의의(균P>0.05).결론 장척농포병환자외주혈TNF-α、IL-17、IL-22표체수평여질병활동상관,제시TNF-α、IL-17화IL-22가능삼여장척농포병적발병과정.
Objective To determine the serum levels of tumor necrosis factor (TNF)-α,interleukin (IL)-17,IL-22 and IL-17F in patients with palmoplantar pustulosis (PP),and to estimate their relationship with disease activity in PP.Methods Venous blood samples were collected from 30 patients with PP at both active stage and stationary stage and from 20 healthy human controls.Enzyme-linked immunosorbent assay (ELISA) was conducted to determine the serum levels of TNF-α,IL-17,IL-22 and IL-17F.The paired Wilcoxon signed rank test was carried out to compare the serum levels of cytokines between patients at active stage and at stationary stage,and the Mann-Whitney U test to compare those among different groups.Results The median serum levels of TNF-α,IL-17 and IL-22 in patients with PP at active stage were 186.35 (range,113.48-412.69) ng/L,420.45 (range,278.55-748.73) ng/L and 106.48 (range,69.13-251.86) ng/L respectively,significantly higher than those at stationary stage (42.52(18.83-95.37) ng/L,48.11 (36.43-80.04) ng/L,20.32 (10.55-48.75) ng/L,respectively,all P < 0.05) and those in the controls (24.30 (12.0-61.56) ng/L,10.49 (6.24-24.44) ng/L,2.58 (1.41-5.78) ng/L,respectively,all P < 0.05).Moreover,the patients at stationary stage showed a significant elevation in serum levels of TNF-α,IL-17 and IL-22 compared with the controls (u =2.71,3.53,2.18,respectively,all P < 0.05).No statistical difference was noted in the serum level of IL-17F among the patients at different stages and controls (P > 0.05).Conclusion The circulating levels of TNF-α,IL-17 and IL-22 were associated with disease activity in PP,hinting that they may be involved in the development of PP.