中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
43期
6895-6899
,共5页
生物材料%材料相容性%氧等离子处理%生物活性玻璃%聚乳酸%细胞相容性%引导骨再生%增殖率%屏障膜材料%碱性磷酸酶%国家自然科学基金
生物材料%材料相容性%氧等離子處理%生物活性玻璃%聚乳痠%細胞相容性%引導骨再生%增殖率%屏障膜材料%堿性燐痠酶%國傢自然科學基金
생물재료%재료상용성%양등리자처리%생물활성파리%취유산%세포상용성%인도골재생%증식솔%병장막재료%감성린산매%국가자연과학기금
背景：目前生物活性玻璃和聚乳酸被应用于口腔科和整形外科临床上，但是由于二者各自的材料特性在临床上应用仍有一定的局限性，治疗效果并不理想。目的：探究氧等离子处理聚乳酸与生物活性玻璃引导骨再生膜细胞相容性的影响。方法：采用生物活性玻璃和聚乳酸为基础制备聚乳酸膜、聚乳酸与生物活性玻璃复合膜、氧等离子处理的聚乳酸与生物活性玻璃复合膜，用以培养MG63细胞，观察3种膜上的MG63细胞的细胞黏附率、增殖率以及碱性磷酸酶活性的差异。结果与结论：随着时间的增长，3组膜的细胞黏附率和增殖率均明显增加，碱性磷酸酶活性均呈先增高后降低的趋势，在培养第7天达峰值。经氧等离子处理聚乳酸与生物活性玻璃组的细胞黏附率和增殖率明显高于其他2组，而聚乳酸组与聚乳酸与生物活性玻璃组细胞黏附和增殖率率接近；而培养第3天时聚乳酸与生物活性玻璃组和氧等离子处理聚乳酸与生物活性玻璃组细胞碱性磷酸酶活性明显高于聚乳酸组，而培养第7，14天时，3组细胞的碱性磷酸酶活性差异无显著性意义。说明经氧等离子处理的聚乳酸与生物活性玻璃复合膜具有良好的生物相容性，可以较好促进细胞黏附、增殖，促进成骨细胞分泌基质。
揹景：目前生物活性玻璃和聚乳痠被應用于口腔科和整形外科臨床上，但是由于二者各自的材料特性在臨床上應用仍有一定的跼限性，治療效果併不理想。目的：探究氧等離子處理聚乳痠與生物活性玻璃引導骨再生膜細胞相容性的影響。方法：採用生物活性玻璃和聚乳痠為基礎製備聚乳痠膜、聚乳痠與生物活性玻璃複閤膜、氧等離子處理的聚乳痠與生物活性玻璃複閤膜，用以培養MG63細胞，觀察3種膜上的MG63細胞的細胞黏附率、增殖率以及堿性燐痠酶活性的差異。結果與結論：隨著時間的增長，3組膜的細胞黏附率和增殖率均明顯增加，堿性燐痠酶活性均呈先增高後降低的趨勢，在培養第7天達峰值。經氧等離子處理聚乳痠與生物活性玻璃組的細胞黏附率和增殖率明顯高于其他2組，而聚乳痠組與聚乳痠與生物活性玻璃組細胞黏附和增殖率率接近；而培養第3天時聚乳痠與生物活性玻璃組和氧等離子處理聚乳痠與生物活性玻璃組細胞堿性燐痠酶活性明顯高于聚乳痠組，而培養第7，14天時，3組細胞的堿性燐痠酶活性差異無顯著性意義。說明經氧等離子處理的聚乳痠與生物活性玻璃複閤膜具有良好的生物相容性，可以較好促進細胞黏附、增殖，促進成骨細胞分泌基質。
배경：목전생물활성파리화취유산피응용우구강과화정형외과림상상，단시유우이자각자적재료특성재림상상응용잉유일정적국한성，치료효과병불이상。목적：탐구양등리자처리취유산여생물활성파리인도골재생막세포상용성적영향。방법：채용생물활성파리화취유산위기출제비취유산막、취유산여생물활성파리복합막、양등리자처리적취유산여생물활성파리복합막，용이배양MG63세포，관찰3충막상적MG63세포적세포점부솔、증식솔이급감성린산매활성적차이。결과여결론：수착시간적증장，3조막적세포점부솔화증식솔균명현증가，감성린산매활성균정선증고후강저적추세，재배양제7천체봉치。경양등리자처리취유산여생물활성파리조적세포점부솔화증식솔명현고우기타2조，이취유산조여취유산여생물활성파리조세포점부화증식솔솔접근；이배양제3천시취유산여생물활성파리조화양등리자처리취유산여생물활성파리조세포감성린산매활성명현고우취유산조，이배양제7，14천시，3조세포적감성린산매활성차이무현저성의의。설명경양등리자처리적취유산여생물활성파리복합막구유량호적생물상용성，가이교호촉진세포점부、증식，촉진성골세포분비기질。
BACKGROUND:Currently, bioactive glass and polylactic acid have been used in clinical dentistry and plastic surgery; however, their therapeutic outcomes are not satisfactory, because the material properties have some limitations. OBJECTIVE:To explore the cytocompatibility of oxygen plasma-treated polylactic acid and bioactive glass guided bone regeneration membrane. METHODS:Bioactive glass and polylactic acid were used as the basic materials to prepare polylactic acid membrane, polylactic acid and bioactive glass composite membrane and oxygen plasme-treated polylactic acid and bioactive glass composite membrane, al of which were used to culture MG63 cels. Cel adhesion rate, cel proliferation rate and alkaline phosphatase activity of MG63 cels on these three kinds of membranes were observed. RESULTS AND CONCLUSION: With the growth of time, in these three groups of membranes, the cel adhesion rate and cel proliferation rate were al significantly increased. Alkaline phosphatase activity showed a decreasing trend after the first increase, and reached its peak at the 7thday of culture. The cel adhesion rate and cel proliferation rate in oxygen plasma-treated polylactic acid and bioactive glass group were significantly higher than those in the other two groups, while the cel adhesion and proliferation rates in polylactic acid and polylactic acid and bioactive glass groups were similar. At the 3rd day of culture, the alkaline phosphatase activity in the polylactic acid and bioactive glass group and oxygen plasma-treated polylactic acid and bioactive glass group was significantly higher than that in the polylactic acid group. At the 7th and 14th days, there was no significant difference in the alkaline phosphatase activity among these three groups. These results show that oxygen plasma-treated polylactic acid and bioactive glass composite membrane has good biocompatibility, which can better promote cel adhesion, proliferation and matrix secretion from osteogenic cels.
