实用肝脏病杂志
實用肝髒病雜誌
실용간장병잡지
Journal of Practical Hepatology
2015年
6期
643-646
,共4页
肝癌%乙酰肝素酶%微血管密度
肝癌%乙酰肝素酶%微血管密度
간암%을선간소매%미혈관밀도
Hepatocellular carcinoma%Heparanase%Microvascular density
目的:观察乙酰肝素酶(Hpa)在肝癌组织中的表达,并探讨其与微血管密度(MVD)的关系。方法采用RT-PCR法检测47例原发性肝癌组织、癌旁组织1(A组)和癌旁组织2(B组)Hpa mRNA水平;采用免疫组织化学法检测肝癌组织Hpa蛋白和CD34表达水平,并分析Hpa蛋白与肝癌临床病理特征的关系,以及Hpa mRNA水平与MVD值的相关性。结果在不同直径和分化程度、不同临床分期、是否转移复发、AFP水平和有无门脉癌栓等肿瘤组织,Hpa蛋白表达存在明显的差异(P<0.05);肝癌组织Hpa mRNA水平(0.793±0.184)和MVD值(34.5±12.2)均明显高于A组和B组(P<0.05),而A组Hpa mRNA水平(0.577±0.145)和MVD值(22.2±10.7)又明显高于B组[(0.384±0.117)和(14.7±7.4),P<0.05];各组Hpa mRNA水平与MVD值呈正相关(r=0.627,P<0.05)。结论Hpa在肝癌的生长、侵袭和血管生成中发挥着重要作用。
目的:觀察乙酰肝素酶(Hpa)在肝癌組織中的錶達,併探討其與微血管密度(MVD)的關繫。方法採用RT-PCR法檢測47例原髮性肝癌組織、癌徬組織1(A組)和癌徬組織2(B組)Hpa mRNA水平;採用免疫組織化學法檢測肝癌組織Hpa蛋白和CD34錶達水平,併分析Hpa蛋白與肝癌臨床病理特徵的關繫,以及Hpa mRNA水平與MVD值的相關性。結果在不同直徑和分化程度、不同臨床分期、是否轉移複髮、AFP水平和有無門脈癌栓等腫瘤組織,Hpa蛋白錶達存在明顯的差異(P<0.05);肝癌組織Hpa mRNA水平(0.793±0.184)和MVD值(34.5±12.2)均明顯高于A組和B組(P<0.05),而A組Hpa mRNA水平(0.577±0.145)和MVD值(22.2±10.7)又明顯高于B組[(0.384±0.117)和(14.7±7.4),P<0.05];各組Hpa mRNA水平與MVD值呈正相關(r=0.627,P<0.05)。結論Hpa在肝癌的生長、侵襲和血管生成中髮揮著重要作用。
목적:관찰을선간소매(Hpa)재간암조직중적표체,병탐토기여미혈관밀도(MVD)적관계。방법채용RT-PCR법검측47례원발성간암조직、암방조직1(A조)화암방조직2(B조)Hpa mRNA수평;채용면역조직화학법검측간암조직Hpa단백화CD34표체수평,병분석Hpa단백여간암림상병리특정적관계,이급Hpa mRNA수평여MVD치적상관성。결과재불동직경화분화정도、불동림상분기、시부전이복발、AFP수평화유무문맥암전등종류조직,Hpa단백표체존재명현적차이(P<0.05);간암조직Hpa mRNA수평(0.793±0.184)화MVD치(34.5±12.2)균명현고우A조화B조(P<0.05),이A조Hpa mRNA수평(0.577±0.145)화MVD치(22.2±10.7)우명현고우B조[(0.384±0.117)화(14.7±7.4),P<0.05];각조Hpa mRNA수평여MVD치정정상관(r=0.627,P<0.05)。결론Hpa재간암적생장、침습화혈관생성중발휘착중요작용。
Objective To explore the expression of heparanase (Hpa) in liver cancer tissues, and its relationship with the microvascular density (MVD). Methods The Hpa mRNA levels in tumor, close-adjacent cancerous (group A) and peri-cancerous liver tissues (group B) from 47 patients with primary liver cancer were detected by RT-PCR. The expression of Hpa protein and CD34 in liver and cancer tissues were detected by immunohistochemistry. The correlation of Hpa mRNA to MVD in tissues with different clinical and pathological characteristics were analyzed. Results There were significant differences between Hpa levels in tumor tissues with different differentiation and clinical stages,with or without metastasis and recurrence,high or low AFP levels,with or without portal vein tumor emboli,and large or small tumor sizes (P<0.05);The Hpa mRNA levels (0.793±0.184) and the MVD (34.5±12.2) in liver cancerous tissues were significantly higher than those in group A and group B (P<0.05),while the Hpa mRNA levels and MVD in group A were significantly higher than those in group B [(0.577±0.145) vs. (0.384±0.117),and (22.2±10.7) vs. (14.7±7.4),respectively,P<0.05];The Hpa mRNA level in each group was positively correlated with MVD (r=0.627,P<0.05). Conclusion Hpa plays an important role in the growth,invasion and angiogenesis of hepatocellular carcinoma.