CAJ | 학술논문

背景：椎体终板由椎体的骨骺骨化形成，脊柱侧凸畸形导致椎体终板承受不正常的应力，此病理情况下椎体终板结构的改变尚未完全明确。 　　目的：使用microCT对大鼠不对称力脊柱侧凸模型椎体终板结构的变化进行观察。 　　方法：选取5周龄雌性SD大鼠15只，手术将钛钉由大鼠L2、L5右侧横突根部置入椎体，并连结镍钛弹簧建立腰椎不对称力脊柱侧凸模型。7周后使用microCT对脊柱侧凸顶点处椎体终板进行扫描分析，Mimics软件对椎体终板骨性结构及内部孔道结构进行3D重建。 　　结果：7周后大鼠脊柱腰段侧凸Cobb角由术后即刻平均25.7°±2.4°进展至平均61.5°±5.4°，microCT平扫重建数据显示脊柱侧凸的凹侧椎体终板较凸侧明显变薄，其内部孔道结构稀疏，骨体积分数（BV/TV）明显增加。 　　结论：腰椎不对称力导致脊柱侧凸的凹侧椎体终板趋向形成一致密的薄骨板，这种病理改变可能对凹侧椎体的生长及椎间盘的营养产生不利影响。
배경：추체종판유추체적골후골화형성，척주측철기형도치추체종판승수불정상적응력，차병리정황하추체종판결구적개변상미완전명학。 　　목적：사용microCT대대서불대칭력척주측철모형추체종판결구적변화진행관찰。 　　방법：선취5주령자성SD대서15지，수술장태정유대서L2、L5우측횡돌근부치입추체，병련결얼태탄황건립요추불대칭력척주측철모형。7주후사용microCT대척주측철정점처추체종판진행소묘분석，Mimics연건대추체종판골성결구급내부공도결구진행3D중건。 　　결과：7주후대서척주요단측철Cobb각유술후즉각평균25.7°±2.4°진전지평균61.5°±5.4°，microCT평소중건수거현시척주측철적요측추체종판교철측명현변박，기내부공도결구희소，골체적분수（BV/TV）명현증가。 　　결론：요추불대칭력도치척주측철적요측추체종판추향형성일치밀적박골판，저충병리개변가능대요측추체적생장급추간반적영양산생불리영향。
Background:The vertebral endplate is formed by epiphyseal ossification of the vertebral body. Abnormal mechanical loading appears in scoliosis as compared to normal spines. How the vertebral endplate was changed in scoliosis is not well understood. Objective:To determine structural changes of vertebral endplate in asymmetric load scoliosis rat models by microCT. Methods:Fifteen 5-week-old female Sprague-Dawley rats were selected to develop lumbar scoliosis by using a nickel-titani-um (NT) coil spring. Two bone screws were implanted in vertebral bodies via the roots of right transverse processes of L2 and L5. The two ends of NT coil spring attached to L2 and L5 screws. MicroCT was used to analyze the vertebral endplate at the curve apex in rat lumbar scoliosis models after 7 weeks and the 3D models of the endplate bone structure and the inter-nal canal structure were reconstructed by Mimics software. Results:The average initial coronal Cobb angle was 25.7?±2.4?immediately after the operation and increased to 61.5?±5.4?on average over 7 weeks. The 3D models showed that the concave side of the scoliotic endplate was thinner than the convex side, the structure of canals was sparse, and the bone volume fraction significantly increased. Conclusions:The concave side of the vertebral endplate tends to form a thin dense bony plate under lumbar asymmetric load. Such pathological changes may adversely affect the growth of concave side of the vertebrae and the intervertebral disc nutrition.