浙江化工
浙江化工
절강화공
Zhejiang Chemical Industry
2015年
10期
51-54
,共4页
RP-HPLC%碱性疏水肽%纯化
RP-HPLC%堿性疏水肽%純化
RP-HPLC%감성소수태%순화
RP-HPLC%alkaline hydrophobic peptide%purification
本研究对象碱性疏水12肽粗品因组分复杂,经反相高效液相色谱纯化后其纯度仅有70%,因此,本研究从纯化过程中的进样量以及梯度方式两个方面进行条件优化,最终可得纯度为96.4%的碱性疏水12肽.
本研究對象堿性疏水12肽粗品因組分複雜,經反相高效液相色譜純化後其純度僅有70%,因此,本研究從純化過程中的進樣量以及梯度方式兩箇方麵進行條件優化,最終可得純度為96.4%的堿性疏水12肽.
본연구대상감성소수12태조품인조분복잡,경반상고효액상색보순화후기순도부유70%,인차,본연구종순화과정중적진양량이급제도방식량개방면진행조건우화,최종가득순도위96.4%적감성소수12태.
In this study, the crude product of 12 peptides was complex and the crude product was com-plex,the purity of the high performance liquid chromatography was only 70%.Therefore, this study was carried out in two aspects: the process of purification, the sample size and the gradient method.Finally, 96.4% of the alkaline hydrophobic 12 peptides can be purified.
