浙江化工
浙江化工
절강화공
Zhejiang Chemical Industry
2015年
10期
9-14
,共6页
虫草素%优化%正交实验
蟲草素%優化%正交實驗
충초소%우화%정교실험
cordyceps%optimization%orthogonal experiment
以啤酒糟为基质,蛹虫草为出发菌株,固态发酵生产虫草菌素. 用单因素法和正交实验对培养基中的含水量、碳氮源及其浓度和无机盐进行优化. 结果表明:培养基70%含水量、2%淀粉、1%蛋白胨、0.05% KH2PO4和0.05% K2HPO4为最优条件. 在优化的发酵条件下,最大虫草素积累量5000.48 μg/g,较优化前提高了133.99%.
以啤酒糟為基質,蛹蟲草為齣髮菌株,固態髮酵生產蟲草菌素. 用單因素法和正交實驗對培養基中的含水量、碳氮源及其濃度和無機鹽進行優化. 結果錶明:培養基70%含水量、2%澱粉、1%蛋白胨、0.05% KH2PO4和0.05% K2HPO4為最優條件. 在優化的髮酵條件下,最大蟲草素積纍量5000.48 μg/g,較優化前提高瞭133.99%.
이비주조위기질,용충초위출발균주,고태발효생산충초균소. 용단인소법화정교실험대배양기중적함수량、탄담원급기농도화무궤염진행우화. 결과표명:배양기70%함수량、2%정분、1%단백동、0.05% KH2PO4화0.05% K2HPO4위최우조건. 재우화적발효조건하,최대충초소적루량5000.48 μg/g,교우화전제고료133.99%.
The beer distiller's grains was used as substrate to produce cordycepin from cordyceps sinen-sis to increase the value of agricultural coproducts. Solid state fermentation(SSF) of cordycepin production in cordyceps sinensis were carried out.The single factor and orthogonal experiments were used to optimize the media conditions of cordyceps militaris. The study was conducted to explore the optimum media conditions to enhance cordycepin production in SSF. The results show that the optimum conditions were as follows:70% moisture content, 2% starch, 1% peptone, 0.05% KH2PO4 and 0.05% K2HPO4, with 5000.48 μg/g,which improved 133.99% than before.
