CAJ | 학술논문

对产L-天冬氨酸酶大肠埃希菌噬菌体进行分离和生理特性研究，有助于为生产过程中噬菌体污染的防治提供指导。采用双层平板法对噬菌体进行分离纯化。利用透射电镜观察噬菌体形态。进行噬菌体全基因组测序和比对。通过测定不同处理条件下噬菌体活性，研究温度、pH、有机溶剂氯仿、去垢剂SDS对噬菌体的影响。从噬菌体污染的L-天冬氨酸酶生产菌种大肠埃希菌HY-05C发酵培养液中分离出1株噬菌体。电镜结果表明，该噬菌体由呈多面体对称的头部和极短的尾部构成。基因组测序和比对结果表明，噬菌体与T7样噬菌体的相似性最高。生理特性研究表明，噬菌体对高温和去垢剂SDS敏感，对有机溶剂氯仿不敏感；最适pH为7.0，碱性条件下活力较为稳定，酸性条件容易失活。噬菌体保藏编号为CICC 80001。
대산L-천동안산매대장애희균서균체진행분리화생리특성연구，유조우위생산과정중서균체오염적방치제공지도。채용쌍층평판법대서균체진행분리순화。이용투사전경관찰서균체형태。진행서균체전기인조측서화비대。통과측정불동처리조건하서균체활성，연구온도、pH、유궤용제록방、거구제SDS대서균체적영향。종서균체오염적L-천동안산매생산균충대장애희균HY-05C발효배양액중분리출1주서균체。전경결과표명，해서균체유정다면체대칭적두부화겁단적미부구성。기인조측서화비대결과표명，서균체여T7양서균체적상사성최고。생리특성연구표명，서균체대고온화거구제SDS민감，대유궤용제록방불민감；최괄pH위7.0，감성조건하활력교위은정，산성조건용역실활。서균체보장편호위CICC 80001。
CarryoutisolationandphysiologicalcharacteristicsstudyonthebacteriophageagainstL-aspartaseprodu-cing E. coli conduces to control the phage contamination during the production process. The phage was isolated and purified adopting double-layer plate method. And the phage morphology was determined using transmission electron microscopy( TEM). The phage omni-genome was sequenced and compared with gene bank. The effects of tempera-ture,pH,organic solvent chloroform and detergent SDS on phage was determined by measuring the phage activity un-der different treatment conditions. A bacteriophage was isolated from L-aspartase producing E. coli strain HY-05C cul-ture broth with phage contamination. The bacteriophage had a symmetrical head of regular polygon and short tails. Ge-nome sequence and comparison results showed that it had a close relationship with T7 like bacteriophage. Research of the bacteriophage physiological characteristics showed that it was sensitive to high temperatures and SDS,but insensi-tive to chloroform,fairly stable in pH 7. 0 and alkaline conditions,however,unstable under acidic condition. Bacterio-phage preservation number is CICC 80001.