CAJ | 학술논문

目的：探讨Ctip2在口颌面部炎性疼痛中的作用。方法：取成年健康雌性SD大鼠132只,随机分为3组；空白对照组大鼠(n=12)不作任何处理,生理盐水(n=60)和福尔马林组(n=60)各大鼠分别在其左侧上唇注射50μL生理盐水( n =60)或等量25 mL/L 的福尔马林。注射结束后立即观察各组大鼠在45 min内的疼痛行为,并分别于注射后30 min及1、2、3、4 h各时间点取各组大鼠的脑干延髓段组织( n=12)；然后分别采用免疫组化染色(n=4)、免疫荧光染色(n=4)以及RT-PCR(n=4)检测各大鼠Vc核中Ctip2的表达情况。结果：①注射25 mL/L福尔马林后的各大鼠均表现出典型的双时相性疼痛反应,生理盐水对照组仅在注射初期出现自发性痛行为反应,空白对照组无类似表现；②注射福尔马林后30 min、1 h和2 h各时间点大鼠Vc核中的 Ctip2阳性颗粒数量均明显增多,且分布集中、染色较深(P<0.05)；注射福尔马林后30 min、1 h各时间点的荧光阳性颗粒均明显增多(P<0.05)；注射福尔马林后30 min时,其Vc核中Ctip2 mRNA表达水平即明显升高,2 h时达到峰值(P<0.05)。结论：Ctip2参与中枢神经系统对炎性疼痛刺激的调节。
목적：탐토Ctip2재구합면부염성동통중적작용。방법：취성년건강자성SD대서132지,수궤분위3조；공백대조조대서(n=12)불작임하처리,생리염수(n=60)화복이마림조(n=60)각대서분별재기좌측상진주사50μL생리염수( n =60)혹등량25 mL/L 적복이마림。주사결속후립즉관찰각조대서재45 min내적동통행위,병분별우주사후30 min급1、2、3、4 h각시간점취각조대서적뇌간연수단조직( n=12)；연후분별채용면역조화염색(n=4)、면역형광염색(n=4)이급RT-PCR(n=4)검측각대서Vc핵중Ctip2적표체정황。결과：①주사25 mL/L복이마림후적각대서균표현출전형적쌍시상성동통반응,생리염수대조조부재주사초기출현자발성통행위반응,공백대조조무유사표현；②주사복이마림후30 min、1 h화2 h각시간점대서Vc핵중적 Ctip2양성과립수량균명현증다,차분포집중、염색교심(P<0.05)；주사복이마림후30 min、1 h각시간점적형광양성과립균명현증다(P<0.05)；주사복이마림후30 min시,기Vc핵중Ctip2 mRNA표체수평즉명현승고,2 h시체도봉치(P<0.05)。결론：Ctip2삼여중추신경계통대염성동통자격적조절。
AIM:To investigate the changes of Ctip2 expression in caudal subnucleus of the spinal trigemi-nal nucleus ( Vc) in rats with formalin-induced orofacial pain . METHODS:132 adult female SD rats were random-ly divided into 3 groups. The rats in control group(n=12) received no injection, in formalin or saline group (n=60) were injected with 50μL of 25 mL/L formalin or the same volume of 0. 9% saline into the left upper lips, respectively. The behavior of the rats was observed for 45 minutes. Their Vc were taken at 30, 60, 120, 180 and 240 minutes after injection respectively, Citp 2 expression in Vc was examined by immunochemistry, immunofluorescence and RT-PCR. RESULTS:The rats scratched the injected sites after administration of formalin, but did not in saline or control groups. Ctip2 protein expression was significantly upregulated in the rats 30 min,1 h and 2 h after formalin injection. Ctip2 mRNA was remarkably increased and reached the peak 2 h after administration of formalin. CONCLUSION:Ctip2 participates in regulating inflammatory pain in central nerve system.