CAJ | 학술논문

茶叶中富含单宁化合物。从分离自黑茶的真菌菌株中，筛选高产单宁酶的菌株；进而分离纯化单宁酶，分析单宁酶对茶汤的转溶效果。从不同产地的3个黑茶样品中，共分离获得44个真菌分离物；经初步鉴定，这些真菌分离物以曲霉属（ Aspergillus）、青霉属（ Penicillium）和散囊菌属（ Eurotium）的真菌居多。以单宁酸为底物的鉴别培养基初筛表明，其中26个真菌分离物在鉴别平板上产生透明圈，显示单宁水解酶活性；通过固体发酵复筛，筛选到1株产单宁酶活性较高的菌株，初步鉴定为青霉属（ Penicillium）菌株，命名为青霉MP-24菌株。青霉MP-24可以以茶叶、茶梗和麸皮等农副产品作为原料固体发酵产生单宁酶。以麸皮为原料的发酵产物经过硫酸铵分级沉淀、DEAE阴离子交换层析和葡聚糖G-150凝胶层析等分离纯化步骤，得到分子量为70 kDa的单一蛋白质条带，单宁酶活力达到603.68 U/mg。纯化获得的单宁酶对茶汤有良好的转溶效果。研究结果表明，在黑茶相关微生物中含有丰富的产单宁酶菌株，是工业酶制剂的重要资源。
다협중부함단저화합물。종분리자흑다적진균균주중，사선고산단저매적균주；진이분리순화단저매，분석단저매대다탕적전용효과。종불동산지적3개흑다양품중，공분리획득44개진균분리물；경초보감정，저사진균분리물이곡매속（ Aspergillus）、청매속（ Penicillium）화산낭균속（ Eurotium）적진균거다。이단저산위저물적감별배양기초사표명，기중26개진균분리물재감별평판상산생투명권，현시단저수해매활성；통과고체발효복사，사선도1주산단저매활성교고적균주，초보감정위청매속（ Penicillium）균주，명명위청매MP-24균주。청매MP-24가이이다협、다경화부피등농부산품작위원료고체발효산생단저매。이부피위원료적발효산물경과류산안분급침정、DEAE음리자교환층석화포취당G-150응효층석등분리순화보취，득도분자량위70 kDa적단일단백질조대，단저매활력체도603.68 U/mg。순화획득적단저매대다탕유량호적전용효과。연구결과표명，재흑다상관미생물중함유봉부적산단저매균주，시공업매제제적중요자원。
Tealeavesarerichintannincompounds.Thepresentstudywastoscreentannasehigh-producingstrains from fungi isolated from Chinese dark tea and proceed to isolate and purify the tannase,analyze the trans-soluble effect of tannase on tea broth. A total of 44 fungal strains were isolated from 3 kinds of Chinese dark tea samples. Most of them were initially identified as members of Aspergillus,Penicillium and Eurotium. Twenty-six isolated fungal strains showed tannic acid hydrolysis activity by producing transparent circle on identification agar plate,which indicated tan-nase producing potential,by using agar plate tests. A fungal strain MP-24 with fairly high tannase activity after re-screening on solid state fermentation was screened,it was initially identified as a strain of Penicillium,and named as Penicillium MP-24 strain. It could produce tannase by solid fermentation using tea leaf,tea stem,wheat bran and oth-er agricultural and side-line products as raw materials. Fermented product using wheat bran as raw material obtained a single protein band of 70 kDa in molecular weight,after precipitated in grades with ammonium sulfate,DEAE-cellu-lose anion exchange chromatography and Sephadex G-150 chromatography and other isolation and purification meas-ures,and the tannase activity was as high as 603. 68 U/mg. The purified tannase had a good trans-soluble effect on tea broth. The results of the study suggested that microbes related to Chinese dark tea are rich in tannin producing strains;it is an important resource of industrial tannase preparation.