CAJ | 학술논문

目的：应用基因芯片技术分析通窍开玄法对鼻窦炎模型大鼠鼻黏膜水通道蛋白（AQP）的影响，并探讨其治疗鼻窦炎的生物学机制。方法：实验大鼠随机分为实验组、模型组和正常组，每组10只，实验组和模型组据 Y.Gel 的改良方法造模，正常组不做任何处理。造模成功后实验组分别给予三和通窍开玄汤低剂量组、三和通窍开玄汤中剂量组、三和通窍开玄汤高剂量组、青霉素 V 钾片灌胃7天，处死动物取鼻黏膜组织，应用 Agi-lent 基因芯片检测各组大鼠鼻黏膜水通道蛋白基因表达，并进行显著性分析。结果：中药各组均上调 AQP1表达，中剂量组、高剂量组又上调 AQP2，青霉素 V 钾组上调 AQP12b 基因；4组均下调 AQP9基因，中药低剂量组还可下调 AQP2、AQP12b，青霉素 V 钾组还可下调 AQP1、AQP2。结论：三和通窍开玄汤与青霉素 V 钾片均对鼻窦炎具良好治疗效果，但调控的 AQP 相关基因表达机制不同，且中药组作用更为广泛，表明对 AQP 相关基因的调控可能是通窍开玄法治疗鼻窦炎生物学机制之一，AQP 是玄府重要实质之一。
목적：응용기인심편기술분석통규개현법대비두염모형대서비점막수통도단백（AQP）적영향，병탐토기치료비두염적생물학궤제。방법：실험대서수궤분위실험조、모형조화정상조，매조10지，실험조화모형조거 Y.Gel 적개량방법조모，정상조불주임하처리。조모성공후실험조분별급여삼화통규개현탕저제량조、삼화통규개현탕중제량조、삼화통규개현탕고제량조、청매소 V 갑편관위7천，처사동물취비점막조직，응용 Agi-lent 기인심편검측각조대서비점막수통도단백기인표체，병진행현저성분석。결과：중약각조균상조 AQP1표체，중제량조、고제량조우상조 AQP2，청매소 V 갑조상조 AQP12b 기인；4조균하조 AQP9기인，중약저제량조환가하조 AQP2、AQP12b，청매소 V 갑조환가하조 AQP1、AQP2。결론：삼화통규개현탕여청매소 V 갑편균대비두염구량호치료효과，단조공적 AQP 상관기인표체궤제불동，차중약조작용경위엄범，표명대 AQP 상관기인적조공가능시통규개현법치료비두염생물학궤제지일，AQP 시현부중요실질지일。
Objective: To analyze the effects of TongQiao KaiXuan method on aquaporin (AQP) of nasal mu-cosa in rat model with nasosinusitis by gene chip technology, and discuss its biological mechanism of treating nasosi-nusitis. Methods: The rats were randomized into the experiment group, the model group and the normal group, the rats in the experiment group and the model group were prepared by Y.Gel reforming method, the normal group were un-handled. After successfully establishing the models, the experiment group accepted intragastric administration of San-He TongQiao KaiXuan Tang in high, moderate and low dose, and phenoxymethylpenicillin potassium tablets for seven consecutive days, nasal mucosa was obtained from the animals, the AQP expressions of the rats in all the groups were detected with Agilent gene chip, and analyzed significantly. Results: AQP1 expressions raised in the groups of TCM, AQP2 expressions raised in moderate and high doses groups, AQP12b gene was up-regulated in phenoxymethylpeni-cillin potassium tablets group; AQP9 gene was down-regulated in four groups, AQP2 and AQP12b decreased in low dose groups of TCM, AQP1 and AQP2 were down-regulated in phenoxymethylpenicillin potassium tablets group. Conclusion: SanHe TongQiao KaiXuan Tang and phenoxymethylpenicillin potassium tablets show better clinical ef-fects in treating nasosinusitis, but the mechanisms of regulating AQP-related gene expression are different, and the functions of TCM groups are extensive, which demonstrates the regulation of AQP-related genes might be one of bio-logical mechanisms of TongQiao KaiXuan method in treating nasosinusitis, AQP is one of the important substances of the sweat pores.