中国循环杂志
中國循環雜誌
중국순배잡지
Chinese Circulation Journal
2015年
10期
971-975
,共5页
罗鹏%庞玲品%吴源聪%陈普文%朱秀龙%陈强%黄石安%何建国
囉鵬%龐玲品%吳源聰%陳普文%硃秀龍%陳彊%黃石安%何建國
라붕%방령품%오원총%진보문%주수룡%진강%황석안%하건국
先天性心脏病相关性肺动脉高压%芳香烃受体
先天性心髒病相關性肺動脈高壓%芳香烴受體
선천성심장병상관성폐동맥고압%방향경수체
Pulmonary arterial hypertension associated with congenital heart disease%Aryl-hydrocarbon receptor
目的:了解先天性心脏病相关性肺动脉高压(CHD-PAH)患者肺组织是否有芳香烃受体(AHR)表达,同时探讨AHR表达量与肺血管重构是否相关。<br> 方法:入选超声心动图和右心导管检查确诊的预行外科修补术的CHD-PAH患者32例。术中行肺组织活检。采用组织免疫荧光检测肺组织标本AHR表达情况,运用图像分析软件计算肺小血管的管壁面积/管总面积(WA/TA)和管壁厚/管外径(WD/TD)2个相对比值,采用实时荧光定量多聚酶链反应(Real-Time PCR)方法检测AHR mRNA、缺氧诱导因子-1α(HIF-1α)mRNA、芳香烃受体核转位蛋白(ARNT)mRNA和血管内皮生长因子(VEGF)mRNA表达情况。此外,术前采集患者外周血,采用酶联免疫吸附法(ELISA)测定血清AHR浓度。<br> 结果:32例患者肺组织中均有AHR表达。且肺组织AHR mRNA表达量与平均肺动脉压(mPAP)(r=0.809, P<0.001)、肺小血管WA/TA(r=0.723,P<0.001)和WD/TD(r=0.746,P<0.001)、HIF-1αmRNA表达量(r=0.889, P<0.001)、ARNT mRNA表达量(r=0.738,P<0.001)、VEGF mRNA表达量(r=0.822,P<0.001)呈正相关。肺组织VEGF mRNA表达量与mPAP(r=0.739,P<0.001)、WD/TD (r=0.702,P<0.001)和WA/TA (r=0.657,P<0.001)呈正相关。血清AHR浓度与mPAP(r=0.754,P<0.001)、WD/TD(r=0.754,P<0.001)和WA/TA (r=0.739,P<0.001)呈正相关。<br> 结论:AHR可能参与CHD-PAH患者的肺血管重构。
目的:瞭解先天性心髒病相關性肺動脈高壓(CHD-PAH)患者肺組織是否有芳香烴受體(AHR)錶達,同時探討AHR錶達量與肺血管重構是否相關。<br> 方法:入選超聲心動圖和右心導管檢查確診的預行外科脩補術的CHD-PAH患者32例。術中行肺組織活檢。採用組織免疫熒光檢測肺組織標本AHR錶達情況,運用圖像分析軟件計算肺小血管的管壁麵積/管總麵積(WA/TA)和管壁厚/管外徑(WD/TD)2箇相對比值,採用實時熒光定量多聚酶鏈反應(Real-Time PCR)方法檢測AHR mRNA、缺氧誘導因子-1α(HIF-1α)mRNA、芳香烴受體覈轉位蛋白(ARNT)mRNA和血管內皮生長因子(VEGF)mRNA錶達情況。此外,術前採集患者外週血,採用酶聯免疫吸附法(ELISA)測定血清AHR濃度。<br> 結果:32例患者肺組織中均有AHR錶達。且肺組織AHR mRNA錶達量與平均肺動脈壓(mPAP)(r=0.809, P<0.001)、肺小血管WA/TA(r=0.723,P<0.001)和WD/TD(r=0.746,P<0.001)、HIF-1αmRNA錶達量(r=0.889, P<0.001)、ARNT mRNA錶達量(r=0.738,P<0.001)、VEGF mRNA錶達量(r=0.822,P<0.001)呈正相關。肺組織VEGF mRNA錶達量與mPAP(r=0.739,P<0.001)、WD/TD (r=0.702,P<0.001)和WA/TA (r=0.657,P<0.001)呈正相關。血清AHR濃度與mPAP(r=0.754,P<0.001)、WD/TD(r=0.754,P<0.001)和WA/TA (r=0.739,P<0.001)呈正相關。<br> 結論:AHR可能參與CHD-PAH患者的肺血管重構。
목적:료해선천성심장병상관성폐동맥고압(CHD-PAH)환자폐조직시부유방향경수체(AHR)표체,동시탐토AHR표체량여폐혈관중구시부상관。<br> 방법:입선초성심동도화우심도관검사학진적예행외과수보술적CHD-PAH환자32례。술중행폐조직활검。채용조직면역형광검측폐조직표본AHR표체정황,운용도상분석연건계산폐소혈관적관벽면적/관총면적(WA/TA)화관벽후/관외경(WD/TD)2개상대비치,채용실시형광정량다취매련반응(Real-Time PCR)방법검측AHR mRNA、결양유도인자-1α(HIF-1α)mRNA、방향경수체핵전위단백(ARNT)mRNA화혈관내피생장인자(VEGF)mRNA표체정황。차외,술전채집환자외주혈,채용매련면역흡부법(ELISA)측정혈청AHR농도。<br> 결과:32례환자폐조직중균유AHR표체。차폐조직AHR mRNA표체량여평균폐동맥압(mPAP)(r=0.809, P<0.001)、폐소혈관WA/TA(r=0.723,P<0.001)화WD/TD(r=0.746,P<0.001)、HIF-1αmRNA표체량(r=0.889, P<0.001)、ARNT mRNA표체량(r=0.738,P<0.001)、VEGF mRNA표체량(r=0.822,P<0.001)정정상관。폐조직VEGF mRNA표체량여mPAP(r=0.739,P<0.001)、WD/TD (r=0.702,P<0.001)화WA/TA (r=0.657,P<0.001)정정상관。혈청AHR농도여mPAP(r=0.754,P<0.001)、WD/TD(r=0.754,P<0.001)화WA/TA (r=0.739,P<0.001)정정상관。<br> 결론:AHR가능삼여CHD-PAH환자적폐혈관중구。
Objective: To study if there is an aryl-hydrocarbon receptor (AHR) expression in patients of pulmonary arterial hypertension associated with congenital heart disease (CHD-PAH) and to explore if the amount of AHR expression related to pulmonary vascular remodeling. <br> Methods:A total of 32 CHD-PAH patients diagnosed by echocardiography and right heart catheterization for surgical repair were enrolled, and the lung tissue biopsy was performed during the operation. The pulmonaryAHR was detected by immunolfuorescence assay, the ratios of vessel wall area/total area (WA/TA) and vessel wall thickness/vessel external diameter (WD/TD) of small pulmonary arteries were calculated with the imaging software, the mRNA expression of AHR, hypoxia-inducible factor-1α (HIF-1α), aryl-hydrocarbon receptor nuclear translocator (ARNT) and vascular endothelial growth factor (VEGF) were examined by RT-PCR. In addition, blood level of AHR was measured by ELISA. <br> Results: There was AHR expression in pulmonary tissue in all 32 patients. And AHR mRNA expressions were positively related to mPAP (r=0.809,P<0.001), WA/TA (r=0.723,P<0.001), WD/TD (r=0.746,P<0.001); and positively related to mRNA expressions of HIF-1α (r=0.889,P<0.001), ARNT (r=0.738,P<0.001), VEGF (r=0.822,P<0.001). Pulmonary tissue VEGF mRNA expressions were positively related to mPAP (r=0.739,P<0.001), WD/TD (r=0.702,P<0.001) and WA/TA (r=0.657,P<0.001). Blood levels of AHR were positively related to mPAP (r=0.754,P<0.001), WD/TD (r=0.754, P<0.001) and WA/TA (r=0.739,P<0.001). <br> Conclusion: AHR might be involved in pulmonary vascular remodeling in CHD-PAHpatients.