CAJ | 학술논문

目的研究GRHL-3在弥漫大B细胞淋巴瘤(DLBCL)组织中的表达及其临床意义.方法收集2006年1月至2011年9月DLBCL患者病理石蜡包埋组织168例,利用免疫组织化学方法检测组织中GRHL-3蛋白的表达.结果 DLBCL非生发中心(non-GCB)型中GRHL-3阳性表达率高于生发中心(GCB)型[84.87％(101/119)比14.29％(7/49),P＜ 0.01].其中,在non-GCB型中临床分期为Ⅲ～Ⅳ期者的GRHL-3阳性表达率高于Ⅰ～Ⅱ期者[90.00％(63/70)比77.56％(38/49),P＜ 0.01];GRHL-3在乳酸脱氢酶升高组中的阳性表达率高于乳酸脱氢酶正常组[91.67％(77/84)比68.57％(24/35),P＜0.01];结外累及≥2处组的阳性表达率高于结外累及0～1处组[96.29％(26/27)比81.52％(75/92),P＜0.05];按国际预后指数(IPI)评分为高危组(IPI 4 ～5分)阳性表达率高于低危组(IPI 0 ～1分)[91.30％(65/69)比66.67％(18/27),P＜ 0.01]及中危组(IPI 2 ～3分)[91.30％(65/69)比79.96％(18/23),P＜ 0.05].但GRHL-3的表达与non-GCB型DLBCL患者性别、年龄及行为状态评分无相关性(P＞0.05).结论 GRHL-3在non-GCB型DLBCL中的阳性表达率明显高于GCB型,而且在non-GCB型中GRHL-3的表达率与Ann Arbor分期、乳酸脱氢酶、结外累及情况及IPI评分有相关性.
목적 연구GRHL-3재미만대B세포림파류(DLBCL)조직중적표체급기림상의의.방법 수집2006년1월지2011년9월DLBCL환자병리석사포매조직168례,이용면역조직화학방법검측조직중GRHL-3단백적표체.결과 DLBCL비생발중심(non-GCB)형중GRHL-3양성표체솔고우생발중심(GCB)형[84.87％(101/119)비14.29％(7/49),P＜ 0.01].기중,재non-GCB형중림상분기위Ⅲ～Ⅳ기자적GRHL-3양성표체솔고우Ⅰ～Ⅱ기자[90.00％(63/70)비77.56％(38/49),P＜ 0.01];GRHL-3재유산탈경매승고조중적양성표체솔고우유산탈경매정상조[91.67％(77/84)비68.57％(24/35),P＜0.01];결외루급≥2처조적양성표체솔고우결외루급0～1처조[96.29％(26/27)비81.52％(75/92),P＜0.05];안국제예후지수(IPI)평분위고위조(IPI 4 ～5분)양성표체솔고우저위조(IPI 0 ～1분)[91.30％(65/69)비66.67％(18/27),P＜ 0.01]급중위조(IPI 2 ～3분)[91.30％(65/69)비79.96％(18/23),P＜ 0.05].단GRHL-3적표체여non-GCB형DLBCL환자성별、년령급행위상태평분무상관성(P＞0.05).결론 GRHL-3재non-GCB형DLBCL중적양성표체솔명현고우GCB형,이차재non-GCB형중GRHL-3적표체솔여Ann Arbor분기、유산탈경매、결외루급정황급IPI평분유상관성.
Objective To study the expression of GRHL-3 in diffuse large B-cell lymphoma (DLBCL) tissues and its clinical significance.Methods One hundred and sixty-eight pathology paraffin-embedded diffuse large B-cell lymphomas tissues were collected from January 2006 to September 2011.Immunohistochemistry was used to detect the expression of GRHL-3 protein in the above tissues.Results The positive expression rate of GRHL-3 protein in the GCB type tissues was higher than that in the non-GCB type tissues [84.87 ％(101/119) vs 14.29 ％ (7/49), P ＜ 0.01].Further analysis indicated that in the non-GCB type tissues,the positive expression rate of GRHL-3 in the latter stage group was significantly higher than that in the early stage group [90.00 ％ (63/70) vs 77.56 ％ (38/49), P ＜ 0.01].The positive expression rate of GRHL-3 in the lactatede hydrogenase increased group was significantly higher than that in the normal lactated hydrogenase [91.67 ％ (77/84) vs 68.57 ％ (24/35), P ＜ 0.01].The positive expression rate of GRHL-3 in the extranodal involvement status ≥ 2 group was significantly higher than that in the extranodal involvement status 0-1 group [96.29 ％ (26/27) vs 81.52 ％ (75/92), P ＜ 0.05].The positive expression rate of GRHL-3 in the IPI score 4-5 group was significantly higher than that in the IPI score 0-1 group [91.30 ％ (65/69) vs 66.67 ％ (18/27), P ＜ 0.01] and IPI score 2-3 group [91.30 ％ (65/69) vs 79.96 ％ (18/23), P ＜ 0.05].However, the expression of GRHL-3 had no correlation with the gender, age, and performance status of DLBCL.Conclusion The positive expression rate of GRHL-3 protein in the GCB type tissues is higher than that in the non-GCB type tissues.The positive expression rate of GRHL-3 in the DLBCL is correlated with the Ann Arbor stage, lactate dehydrogenase, extranodal involvement status and IPI score.