CAJ | 학술논문

目的探索社区获得性肺炎蛋白指纹图谱的特点. 方法从本院临床病例中,选择社区获得性细菌性肺炎患者与健康者各60例,进行血清蛋白指纹图谱检测,分析其相关蛋白峰值并进行统计学处理.结果对60例社区获得性细菌性肺炎患者与60例健康者的血清蛋白指纹图谱数据进行比较,发现有3个蛋白峰(1028. 49、4796. 56、7564. 77m/z)存在显著的差异(P<0. 01). 由此3个蛋白峰组成的诊断模型判别社区获得性细菌性肺炎的总有效率为95. 8%(115/120),特异度为92. 3%(60/65)、灵敏度为100%(55/55)、阳性预测值为91. 7%(55/60)、阴性预测值为100%(60/60). 结论蛋白质指纹图谱技术具有方法简便、检测快速,标本用量少等优点,可能成为社区获得性肺炎早期诊断的辅助指标.
목적 탐색사구획득성폐염단백지문도보적특점. 방법 종본원림상병례중,선택사구획득성세균성폐염환자여건강자각60례,진행혈청단백지문도보검측,분석기상관단백봉치병진행통계학처리.결과 대60례사구획득성세균성폐염환자여60례건강자적혈청단백지문도보수거진행비교,발현유3개단백봉(1028. 49、4796. 56、7564. 77m/z)존재현저적차이(P<0. 01). 유차3개단백봉조성적진단모형판별사구획득성세균성폐염적총유효솔위95. 8%(115/120),특이도위92. 3%(60/65)、령민도위100%(55/55)、양성예측치위91. 7%(55/60)、음성예측치위100%(60/60). 결론 단백질지문도보기술구유방법간편、검측쾌속,표본용량소등우점,가능성위사구획득성폐염조기진단적보조지표.
Objective To explore the characteristics of community acquired pneumonia using proteinfingerprinting technology. Method 60 patients with community acquired bacterial pneumonia, and 60 healthy volunteers were se-lected from known clinical cases. Serum protein fingerprint studies were used to analyze their protein peaks and per-form statistical processing. Result Comparison of the serum protein fingerprinting data from the pool of 60 patients and 60 healthy volunteers, Significant difference in 3 protein peaks(1028. 49、4796. 56、7564. 77 m/z)identified be-tween community acquired bacterial pneumonia and healthy volunteers (P<0. 01). The total effective rate of the 3 protein peaks as a diagnosis model for differential diagnosis of community acquired bacterial pneumonia and healthy volunteers was 95. 8%(115/120), The specificity was 92. 3%(60/65),the sensitivity was 100%(55/55), The positive predictive value was 91. 7%(55/60), the negative predictive value was 100%(60/60). Conclusion Pro-tein fingerprinting technology is advantageous of being a simple method, quick detection, and requires less amount of sample. It is expected to become the early auxiliary diagnosis index for differential diagnosis of community ac-quired pneumonia.