电针合谷穴对实验性牙髓痛大鼠三叉神经节P2X2、P2X3受体的影响
전침합곡혈대실험성아수통대서삼차신경절P2X2、P2X3수체적영향
Effect of Electroacupuncture at Hegu (LI 4) on P2X2 and P2X3 Receptors in Trigeminal Ganglion in Pulpalgia Rats
  • 万方数据
    上海针灸杂志 상해침구잡지
    Shanghai Journal of Acupuncture and Moxibustion
    2015年 11期 1117-1121 ,共5页

    陈莉%张英%严琴%乐凯

    진리%장영%엄금%악개

    电针%P2X2受体%P2X3受体%牙髓痛%穴,合谷%A-317491%大鼠 電針%P2X2受體%P2X3受體%牙髓痛%穴,閤穀%A-317491%大鼠
    전침%P2X2수체%P2X3수체%아수통%혈,합곡%A-317491%대서
    Electroacupuncture%P2X2 receptor%P2X3 receptor%Pulpalgia%Point,Hegu (LI 4)%A-317491%Rats
    目的：探讨电针合谷穴对实验性牙髓痛大鼠三叉神经节P2X2、P2X3受体表达的影响。方法将42只成年雄性SD大鼠随机分为正常组(N组)、对照组(C组)、牙髓痛组(M组)、拮抗剂组(A组)、电针组(E组)和拮抗剂+电针组(AE组),每组7只。N组不做任何处理；C组在钻好的牙髓腔内注入与M组等量的生理盐水,浸润5～6 min后用牙科填料将牙洞封闭；M组用小型电钻或手钻(钻头直径约1 mm)在上颌一侧第一和第二磨牙上钻孔,深入牙髓腔并暴露牙髓,用微量注射器注入浓度为5μg/μL的大肠杆菌内毒素(LPS)溶液(每孔注入1～3μL),浸润5～6 min后用牙科填料将牙洞封闭；A组造模方法同M组,在注射器注入LPS溶液时,将A-317491(0.5 mg/kg)同时注射进牙髓；E组电针双侧合谷穴,留针30 min,每日1次,共治疗3次；AE组按A组造模后按E组方法治疗。每日治疗后观察大鼠的行为学变化及体重变化30 min。第4天处死所有大鼠,运用RT-PCR方法检测大鼠三叉神经节P2X2、P2X3受体及β-actin的基因(mRNA)表达,比较各组P2X2、P2X3受体mRNA相对表达量。结果 M组、A组、E组和AE组行为学变化均显著高于C组和N组(P＜0.01)；A组、E组和AE组行为学变化均显著低于M组(P＜0.01)。C组体重显著低于N组(P＜0.01)；M组、A组、E组和AE组体重均显著低于C组和N组(P＜0.01)；E组、AE组体重均显著高于M组和A组(P＜0.01,P＜0.05)；A组体重稍高于M组(P＜0.05)；AE组体重显著高于E组(P＜0.01)。M组、A组、E组和AE组P2X2受体mRNA表达量均显著高于N组和C组(P＜0.01)；A组、E组和AE组P2X2受体mRNA表达量低于M组(P＜0.05)；A组P2X2受体mRNA表达量低于E组(P＜0.05)；E组P2X2受体mRNA表达量高于AE组(P＜0.05)。M组、A组和E组P2X3受体mRNA表达量均高于C组(P＜0.05),且显著高于N组(P＜0.01)；A组、E组和AE组P2X3受体mRNA表达量显著低于M组(P＜0.01)；AE组P2X3受体mRNA表达量明显低于E组(P＜0.01)。结论 LPS诱导大鼠牙髓痛时,三叉神经节P2X2、P2X3受体蛋白表达量增加。电针合谷穴和注射A-317491均可降低LPS诱导牙髓痛大鼠P2X2、P2X3受体mRNA表达,这可能是电针合谷穴镇痛的机制。
    목적：탐토전침합곡혈대실험성아수통대서삼차신경절P2X2、P2X3수체표체적영향。방법장42지성년웅성SD대서수궤분위정상조(N조)、대조조(C조)、아수통조(M조)、길항제조(A조)、전침조(E조)화길항제+전침조(AE조),매조7지。N조불주임하처리；C조재찬호적아수강내주입여M조등량적생리염수,침윤5～6 min후용아과전료장아동봉폐；M조용소형전찬혹수찬(찬두직경약1 mm)재상합일측제일화제이마아상찬공,심입아수강병폭로아수,용미량주사기주입농도위5μg/μL적대장간균내독소(LPS)용액(매공주입1～3μL),침윤5～6 min후용아과전료장아동봉폐；A조조모방법동M조,재주사기주입LPS용액시,장A-317491(0.5 mg/kg)동시주사진아수；E조전침쌍측합곡혈,류침30 min,매일1차,공치료3차；AE조안A조조모후안E조방법치료。매일치료후관찰대서적행위학변화급체중변화30 min。제4천처사소유대서,운용RT-PCR방법검측대서삼차신경절P2X2、P2X3수체급β-actin적기인(mRNA)표체,비교각조P2X2、P2X3수체mRNA상대표체량。결과 M조、A조、E조화AE조행위학변화균현저고우C조화N조(P＜0.01)；A조、E조화AE조행위학변화균현저저우M조(P＜0.01)。C조체중현저저우N조(P＜0.01)；M조、A조、E조화AE조체중균현저저우C조화N조(P＜0.01)；E조、AE조체중균현저고우M조화A조(P＜0.01,P＜0.05)；A조체중초고우M조(P＜0.05)；AE조체중현저고우E조(P＜0.01)。M조、A조、E조화AE조P2X2수체mRNA표체량균현저고우N조화C조(P＜0.01)；A조、E조화AE조P2X2수체mRNA표체량저우M조(P＜0.05)；A조P2X2수체mRNA표체량저우E조(P＜0.05)；E조P2X2수체mRNA표체량고우AE조(P＜0.05)。M조、A조화E조P2X3수체mRNA표체량균고우C조(P＜0.05),차현저고우N조(P＜0.01)；A조、E조화AE조P2X3수체mRNA표체량현저저우M조(P＜0.01)；AE조P2X3수체mRNA표체량명현저우E조(P＜0.01)。결론 LPS유도대서아수통시,삼차신경절P2X2、P2X3수체단백표체량증가。전침합곡혈화주사A-317491균가강저LPS유도아수통대서P2X2、P2X3수체mRNA표체,저가능시전침합곡혈진통적궤제。
    Objective To explore the effect of electroacupuncture at Hegu (LI 4) on the expressions of P2X2 and P2X3 receptors in experimental pulpalgia rats.Method Forty-two male SD rats were randomized into a normal group (group N), a control group (group C), a pulpalgia model group (group M), an antagonist group (group A), an electroacupuncture group (group E), and an antagonist+electroacupuncture group (group AE), 7 rats in each group. Group N didn’t receive any interventions; group C received injection of normal saline into pulp cavity of the same dose as the injection in group M, and the cavity was then blocked by dental fillings 5-6 min later; in group M, maxillary first and second molar teeth were drilled (drill bit of 1 mm in diameter) to expose pulp and lipopolysaccharide (LPS) solution 5μg/μL was injected into the holes (1~3μL for each hole), and the holes were then covered by dental fillings 5-6 min later; group A received the same modeling method as that in group M, but A-317491 was injected together with LPS (0.5 mg/kg); group E received electroacupuncture at bilateral Hegu (LI 4) with needles retained for 30 min, once a day, totally for 3 times; group AE received the same electroacupuncture intervention after receiving the same treatments as that in group A. The rats’ behaviors and weight were observed for 30 min after intervention each day. The rats were sacrificed on the 4th day, and the mRNA expressions of P2X2 and P2X3 receptors andβ-actin in trigeminal ganglion were detected by using RT-PCR. The mRNA expressions were then compared among the groups.Result The behavioral changes in group M, E, and AE were more significant than that in group C and N (P<0.01); the behavioral changes in group A, E, and AE were less significant than that in group M (P<0.01). The weight in group C was significantly lower than that in group N (P<0.01); the weights in group M, A, E and AE were significantly lower than that in group C and N (P<0.01); the weights in group E and AE were significantly higher than that in group M and A (P<0.01,P<0.05); the weight in group A was slightly higher than that in group M (P<0.05); the weights in group AE was significantly higher than that in group E (P<0.01). The mRNA expressions of P2X2 receptor in group M, A, and AE were significantly higher than that in group N and C (P<0.01); the mRNA expressions of P2X2 receptor in group A, E, and AE were lower than that in group M (P<0.05); the mRNA expression of P2X2 receptor in group A was lower than that in group E (P<0.05); the mRNA expression of P2X2 receptor in group E was higher than that in group AE (P<0.05). The mRNA expressions of P2X3 receptor in group M, A, and E were significantly higher than that in group C (P<0.05) and group N (P<0.01); the mRNA expressions of P2X3 receptor in group A, E, and AE were significantly lower than that in group M (P<0.01); the mRNA expression of P2X3 receptor in group AE was markedly lower than that in group E (P<0.01).Conclusion The expressions of P2X2 and P2X3 receptors in trigeminal ganglion were increased in LPS-induced pulpalgia rats. Electroacupuncture at Hegu (LI 4) and injection of A-317491 both can down-regulate the mRNA expressions of P2X2 and P2X3 receptors, which is plausibly the action mechanism of electroacupuncture at Hegu (LI 4) in analgesia.
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