听力学及言语疾病杂志
聽力學及言語疾病雜誌
은역학급언어질병잡지
Journal of Audiology and Speech Pathology
2015年
6期
622-625
,共4页
西地那非%噪声性聋%毛细胞
西地那非%譟聲性聾%毛細胞
서지나비%조성성롱%모세포
Sildenafil%Noise-induced hearing loss%Hair cell
目的：探讨5－磷酸二酯酶（phosphodiesterasetype 5，PDE5）抑制剂西地那非对噪声性聋豚鼠耳蜗显微结构的影响。方法30只豚鼠随机分为对照组、噪声暴露组和PD E5抑制剂组，每组10只。PD E5抑制剂组和噪声暴露组豚鼠在110 dB SPL白噪声暴露2周后分别腹腔注射西地那非10 mg?kg －1?d－1及生理盐水4 ml?kg －1?d－1，连续给药4周；对照组安静环境饲养，不给予噪声暴露及任何药物。分别于噪声暴露前1天、噪声暴露后给药前及给药第1、2及4周各组行ABR检测，并通过光镜观察噪声暴露后4周豚鼠耳蜗外毛细胞缺失率变化。结果与噪声暴露前相比，PDE5抑制剂组和噪声暴露组ABR反应阈明显升高，波I潜伏期明显延长，但PDE5抑制剂组的增幅均小于噪声暴露组，差异有统计学意义（P＜0．01）。正常对照组外毛细胞无明显缺失，噪声暴露组和PDE5抑制组外毛细胞缺失率分别为39．30％±5．69％和37．13％±7．81％，两组间差异无统计学意义（P＞0．05）。结论 PD E5抑制剂不能促使坏死脱落的毛细胞再生，但能在一定程度上恢复噪声性听力损失豚鼠的听功能。
目的：探討5－燐痠二酯酶（phosphodiesterasetype 5，PDE5）抑製劑西地那非對譟聲性聾豚鼠耳蝸顯微結構的影響。方法30隻豚鼠隨機分為對照組、譟聲暴露組和PD E5抑製劑組，每組10隻。PD E5抑製劑組和譟聲暴露組豚鼠在110 dB SPL白譟聲暴露2週後分彆腹腔註射西地那非10 mg?kg －1?d－1及生理鹽水4 ml?kg －1?d－1，連續給藥4週；對照組安靜環境飼養，不給予譟聲暴露及任何藥物。分彆于譟聲暴露前1天、譟聲暴露後給藥前及給藥第1、2及4週各組行ABR檢測，併通過光鏡觀察譟聲暴露後4週豚鼠耳蝸外毛細胞缺失率變化。結果與譟聲暴露前相比，PDE5抑製劑組和譟聲暴露組ABR反應閾明顯升高，波I潛伏期明顯延長，但PDE5抑製劑組的增幅均小于譟聲暴露組，差異有統計學意義（P＜0．01）。正常對照組外毛細胞無明顯缺失，譟聲暴露組和PDE5抑製組外毛細胞缺失率分彆為39．30％±5．69％和37．13％±7．81％，兩組間差異無統計學意義（P＞0．05）。結論 PD E5抑製劑不能促使壞死脫落的毛細胞再生，但能在一定程度上恢複譟聲性聽力損失豚鼠的聽功能。
목적：탐토5－린산이지매（phosphodiesterasetype 5，PDE5）억제제서지나비대조성성롱돈서이와현미결구적영향。방법30지돈서수궤분위대조조、조성폭로조화PD E5억제제조，매조10지。PD E5억제제조화조성폭로조돈서재110 dB SPL백조성폭로2주후분별복강주사서지나비10 mg?kg －1?d－1급생리염수4 ml?kg －1?d－1，련속급약4주；대조조안정배경사양，불급여조성폭로급임하약물。분별우조성폭로전1천、조성폭로후급약전급급약제1、2급4주각조행ABR검측，병통과광경관찰조성폭로후4주돈서이와외모세포결실솔변화。결과여조성폭로전상비，PDE5억제제조화조성폭로조ABR반응역명현승고，파I잠복기명현연장，단PDE5억제제조적증폭균소우조성폭로조，차이유통계학의의（P＜0．01）。정상대조조외모세포무명현결실，조성폭로조화PDE5억제조외모세포결실솔분별위39．30％±5．69％화37．13％±7．81％，량조간차이무통계학의의（P＞0．05）。결론 PD E5억제제불능촉사배사탈락적모세포재생，단능재일정정도상회복조성성은력손실돈서적은공능。
Objective To study the effect of the PDE5 inhibitor on noise-induced hearing loss .Methods 30 guinea pigs were randomly divided into control group ,noise exposure group and PDE5 inbibiter group(10 in each) . Two weeks after white noise exposure of 110 dB ,sildenafil (10 mg?kg -1 ?d-1 ) and NS(4 ml?kg -1 ?d-1 ) were injected into guinea pigs of PDE5 inbibiter group and the noise group respectively .One week after noise exposure four weeks continuous administration was cavried out .The ABR thresholds and the PL of wave Ⅰ were measured respectively prior to the experiment ,1 week post-noise ,1 ,2 and 4 weeks post-drugs .The changes of cochlea hair cells were also observed by light microscopy .Results The ABR threshold shifts and the PL in sildenafil group were less obvious than that of in the noise group .The number of OHCs loss was relatively stable in the normal group , while the obvious OHC loss was observed in other groups .There was significant difference among the three groups , however ,the OHC loss in the sildenafil treatment group was not significantly different from that in the noise expo‐sure (P>0 .05) .Conclusion The inhibitor of PDE5 is able to reduce the decrease of ABR thresholds shift ,shorten the extension of the PL ,and it can significantly protect against noise-induced hearing loss .
