CAJ | 학술논문

传统的羽绒品种定量检测方法为显微镜检测法,费时长且依赖检测人员的经验.本研究建立了基于实时荧光PCR技术的鹅鸭混合绒定量检测方法.通过设计针对鹅、鸭线粒体DNA序列的特异性引物和TaqMan探针,且两组引物探针具有几近相同的扩增效率,建立羽绒品种检测的定量标准曲线(R2>0.99).以不同产地的鹅鸭混合绒验证该方法,均得到较好结果,证明线粒体拷贝数对定量结果基本没有影响.该方法有望用于羽绒品种的大批量快速定量检测.
전통적우융품충정량검측방법위현미경검측법,비시장차의뢰검측인원적경험.본연구건립료기우실시형광PCR기술적아압혼합융정량검측방법.통과설계침대아、압선립체DNA서렬적특이성인물화TaqMan탐침,차량조인물탐침구유궤근상동적확증효솔,건립우융품충검측적정량표준곡선(R2>0.99).이불동산지적아압혼합융험증해방법,균득도교호결과,증명선립체고패수대정량결과기본몰유영향.해방법유망용우우융품충적대비량쾌속정량검측.
In this project, a quantitative method based on real-time PCR technique was developed to determine the weight of goose down and duck down in down products. Two sets of primers and TaqMan probes that can react specifically to goose and duck mitochondrial DNA were designed, and they exert almost the same amplification rate. The two systems were used to establish the quantitative standard curve of down species determination(R2>0.99). Goose and duck down blends collected from different producing areas were employed to verify the method, and satisfactory results were obtained, which proved that the copy number of mitochondrion has little impact on quantifying results. The method can be applied to quick and large-batch quantitative identification of down species in down products.