西部中医药
西部中醫藥
서부중의약
Western Journal of Traditional Chinese Medicine
2015年
10期
46-48
,共3页
愈伤胶囊%三七总皂苷%延胡索乙素%质量标准%高效液相色谱
愈傷膠囊%三七總皂苷%延鬍索乙素%質量標準%高效液相色譜
유상효낭%삼칠총조감%연호색을소%질량표준%고효액상색보
YuShang capsules%Panax Notoginseng Saponins%tetrahydropalmatine%quality standard%HPLC
目的:通过测定愈伤胶囊中三七总皂苷和延胡素乙素的含量,建立愈伤胶囊质量控制项下的含量测定标准。方法:采用高效液相色谱(HPLC)方法,Waters公司的 Sunfire C18色谱柱(150 mm×4.6 mm,5μm)。人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1以乙腈为流动相A,以水为流动相B,二元梯度洗脱,0~30分钟,18%~19%A;30~40分钟,19%~31%A;40~60分钟,31%~56%A,检测波长203 nm,流速为1.5 mL/min;延胡索乙素以甲醇-0.1%磷酸溶液(三乙胺调ph值至6.0)(55∶45)为流动相;检测波长为280 nm。结果:经测定愈伤胶囊粉末中,每克按干燥品计,三七皂苷R1、人参皂苷Rb1、人参皂苷Rg1的总皂苷含量为0.46 mg/g,延胡索乙素含量为0.046 mg/g。结论:HPLC法测定愈伤胶囊质量三七总皂苷含量与延胡索乙素准确可行,可作为愈伤胶囊含量控制的指标性成分。
目的:通過測定愈傷膠囊中三七總皂苷和延鬍素乙素的含量,建立愈傷膠囊質量控製項下的含量測定標準。方法:採用高效液相色譜(HPLC)方法,Waters公司的 Sunfire C18色譜柱(150 mm×4.6 mm,5μm)。人參皂苷Rg1、人參皂苷Rb1、三七皂苷R1以乙腈為流動相A,以水為流動相B,二元梯度洗脫,0~30分鐘,18%~19%A;30~40分鐘,19%~31%A;40~60分鐘,31%~56%A,檢測波長203 nm,流速為1.5 mL/min;延鬍索乙素以甲醇-0.1%燐痠溶液(三乙胺調ph值至6.0)(55∶45)為流動相;檢測波長為280 nm。結果:經測定愈傷膠囊粉末中,每剋按榦燥品計,三七皂苷R1、人參皂苷Rb1、人參皂苷Rg1的總皂苷含量為0.46 mg/g,延鬍索乙素含量為0.046 mg/g。結論:HPLC法測定愈傷膠囊質量三七總皂苷含量與延鬍索乙素準確可行,可作為愈傷膠囊含量控製的指標性成分。
목적:통과측정유상효낭중삼칠총조감화연호소을소적함량,건립유상효낭질량공제항하적함량측정표준。방법:채용고효액상색보(HPLC)방법,Waters공사적 Sunfire C18색보주(150 mm×4.6 mm,5μm)。인삼조감Rg1、인삼조감Rb1、삼칠조감R1이을정위류동상A,이수위류동상B,이원제도세탈,0~30분종,18%~19%A;30~40분종,19%~31%A;40~60분종,31%~56%A,검측파장203 nm,류속위1.5 mL/min;연호색을소이갑순-0.1%린산용액(삼을알조ph치지6.0)(55∶45)위류동상;검측파장위280 nm。결과:경측정유상효낭분말중,매극안간조품계,삼칠조감R1、인삼조감Rb1、인삼조감Rg1적총조감함량위0.46 mg/g,연호색을소함량위0.046 mg/g。결론:HPLC법측정유상효낭질량삼칠총조감함량여연호색을소준학가행,가작위유상효낭함량공제적지표성성분。
Objective:To establish the standard of the content determination of YuShang capsules for quality control by detecting the contents of Panax Notoginseng Saponins (PNS) and tetrahydropalmatine in YuShang cap-sules. Methods:HPLC method was adopted, Sunfire C18 chromatographic column (150 mm×4.6 mm, 5μm). Gin-senoside Rg1, Ginsenoside Rb1, PNS R1 with acetonitrile as mobile phase A and water as mobile phase B, dual gra-dient elution, 0~30 minutes, 18%~19%A; 30~40 minutes, 19%~31%A; 40~60 minutes, 31%~56%A, detection wavelength was 203 nm, flow rate was 1.5mL/min;methanol-0.1%phosphoric acid solution(ph of triethylamine was adjusted to 6.0)(55:45) were taken as mobile phase for tetrahydropalmatine;detection wavelength was 280 nm. Re-sults:The contents of total saponins and tetrahydropalmatine in PNS R1, Ginsenoside Rb1 and ginsenoside Rg1 con-tained in YuShang capsules after detected in the powder of dry produce per gram. Conclusion:HPLC method used to detect PNS and tetrahydropalmatine in YuShang capsules is accurate and feasible, which could be used as indicative indexes of YuShang capsules content control.