西部中医药
西部中醫藥
서부중의약
Western Journal of Traditional Chinese Medicine
2015年
10期
49-52
,共4页
郭鸿雁%冷晓红%陈海燕%郝彩琴
郭鴻雁%冷曉紅%陳海燕%郝綵琴
곽홍안%랭효홍%진해연%학채금
苦豆子籽%大孔树脂%分离纯化
苦豆子籽%大孔樹脂%分離純化
고두자자%대공수지%분리순화
the seeds of KuDouZi%macroporous resin%separation and purification
目的：选用大孔树脂纯化苦豆子籽提取液的生物碱的纯度，提高生物碱的纯度。方法：pH=9，浓度为2.0～2.5 mg/mL的料液，抽滤加氯化钠(1.0 mol/L)，以树脂重量与样品的体积比为1∶3上柱，用3倍样品体积pH=9的30%乙醇先洗脱，再用3倍样品体积pH=9为70%乙醇洗脱。结论：选用非极性D101大孔树脂直接从苦豆子籽提取液中分离纯化生物终产品总碱纯度可达60%以上，洗脱率为97%以上，分离效果好。
目的：選用大孔樹脂純化苦豆子籽提取液的生物堿的純度，提高生物堿的純度。方法：pH=9，濃度為2.0～2.5 mg/mL的料液，抽濾加氯化鈉(1.0 mol/L)，以樹脂重量與樣品的體積比為1∶3上柱，用3倍樣品體積pH=9的30%乙醇先洗脫，再用3倍樣品體積pH=9為70%乙醇洗脫。結論：選用非極性D101大孔樹脂直接從苦豆子籽提取液中分離純化生物終產品總堿純度可達60%以上，洗脫率為97%以上，分離效果好。
목적：선용대공수지순화고두자자제취액적생물감적순도，제고생물감적순도。방법：pH=9，농도위2.0～2.5 mg/mL적료액，추려가록화납(1.0 mol/L)，이수지중량여양품적체적비위1∶3상주，용3배양품체적pH=9적30%을순선세탈，재용3배양품체적pH=9위70%을순세탈。결론：선용비겁성D101대공수지직접종고두자자제취액중분리순화생물종산품총감순도가체60%이상，세탈솔위97%이상，분리효과호。
Objective:To increase the purity of alkaloids by applying macroporous resin to total alkaloids from the extract of KuDouZi (Sophora alopecuroides L) seeds. Methods:Feed liquid, scaling pH=9, in the concentrations of 2.0 and 2.5 mg/mL, leaching and adding sodium chloride (1.0 mol/L), the weight of macroporous resin and the volume of the samples were in the ratio of 1:3 on the column, three times of the volume of the samples, pH=9, were used to elute firstly, and then three times volume of 70%ethanol with pH=9 were used. Conclusion:Non-polar D101 macroporous resin is chosen to separate and purify total alkaloids of the extract of KuDouZi seeds directly, which could make the purity of total alkaloids more than 60%, elution rate higher than 97%and obtaining good separation effects.
