草业学报
草業學報
초업학보
Acta Prataculturae Sinica
2015年
11期
82-90
,共9页
匍匐翦股颖%2,3-丁二醇%2R,3R-丁二醇%褐斑病
匍匐翦股穎%2,3-丁二醇%2R,3R-丁二醇%褐斑病
포복전고영%2,3-정이순%2R,3R-정이순%갈반병
creeping bentgrass%2,3-BD%2R,3R-BD%Rhizoctonia solani
用250μmol／L 的2，3-丁二醇（2，3-BD）与100μmol／L 的2R，3R-丁二醇（2R，3R-BD）注射至匍匐翦股颖根部后接种立枯丝核菌，诱导匍匐翦股颖对褐斑病的抗性。测定两诱导剂处理对立枯丝核菌发病率的影响，分析诱导后匍匐翦股颖叶片抗坏血酸－谷胱甘肽循环中关键酶活性及氧化还原水平变化情况，确定2，3-BD 与2R，3R-BD在诱导匍匐翦股颖抗病性过程中，抗坏血酸－谷胱甘肽循环的变化及其与抗病性的相关性。结果表明，2，3-BD 与2R，3R-BD 处理匍匐翦股颖后明显降低接菌后的病叶率，同时叶片抗坏血酸过氧化物酶（APX）活性增幅低于对照，谷胱甘肽还原酶（GR）活性提高，还原型抗坏血酸（AsA）含量呈前期减少后期增加趋势，脱氢抗坏血酸（DHA）含量在第1，9天出现两次高峰，与对照相比显著提高，且两处理的 AsA／DHA 在第5天达到最大值，分别为对照的5．0，3．4倍，还原型谷胱甘肽（GSH）含量显著提高，并且两处理的 GSH 与氧化型谷胱甘肽（GSSG）比值在第9天达到最大值，分别为对照的2．34，1．66倍。2，3-BD 与2R，3R-BD 诱导匍匐翦股颖抗褐斑病的过程中，抗坏血酸－谷胱甘肽循环维持较高效率参与植物抗病反应。
用250μmol／L 的2，3-丁二醇（2，3-BD）與100μmol／L 的2R，3R-丁二醇（2R，3R-BD）註射至匍匐翦股穎根部後接種立枯絲覈菌，誘導匍匐翦股穎對褐斑病的抗性。測定兩誘導劑處理對立枯絲覈菌髮病率的影響，分析誘導後匍匐翦股穎葉片抗壞血痠－穀胱甘肽循環中關鍵酶活性及氧化還原水平變化情況，確定2，3-BD 與2R，3R-BD在誘導匍匐翦股穎抗病性過程中，抗壞血痠－穀胱甘肽循環的變化及其與抗病性的相關性。結果錶明，2，3-BD 與2R，3R-BD 處理匍匐翦股穎後明顯降低接菌後的病葉率，同時葉片抗壞血痠過氧化物酶（APX）活性增幅低于對照，穀胱甘肽還原酶（GR）活性提高，還原型抗壞血痠（AsA）含量呈前期減少後期增加趨勢，脫氫抗壞血痠（DHA）含量在第1，9天齣現兩次高峰，與對照相比顯著提高，且兩處理的 AsA／DHA 在第5天達到最大值，分彆為對照的5．0，3．4倍，還原型穀胱甘肽（GSH）含量顯著提高，併且兩處理的 GSH 與氧化型穀胱甘肽（GSSG）比值在第9天達到最大值，分彆為對照的2．34，1．66倍。2，3-BD 與2R，3R-BD 誘導匍匐翦股穎抗褐斑病的過程中，抗壞血痠－穀胱甘肽循環維持較高效率參與植物抗病反應。
용250μmol／L 적2，3-정이순（2，3-BD）여100μmol／L 적2R，3R-정이순（2R，3R-BD）주사지포복전고영근부후접충립고사핵균，유도포복전고영대갈반병적항성。측정량유도제처리대립고사핵균발병솔적영향，분석유도후포복전고영협편항배혈산－곡광감태순배중관건매활성급양화환원수평변화정황，학정2，3-BD 여2R，3R-BD재유도포복전고영항병성과정중，항배혈산－곡광감태순배적변화급기여항병성적상관성。결과표명，2，3-BD 여2R，3R-BD 처리포복전고영후명현강저접균후적병협솔，동시협편항배혈산과양화물매（APX）활성증폭저우대조，곡광감태환원매（GR）활성제고，환원형항배혈산（AsA）함량정전기감소후기증가추세，탈경항배혈산（DHA）함량재제1，9천출현량차고봉，여대조상비현저제고，차량처리적 AsA／DHA 재제5천체도최대치，분별위대조적5．0，3．4배，환원형곡광감태（GSH）함량현저제고，병차량처리적 GSH 여양화형곡광감태（GSSG）비치재제9천체도최대치，분별위대조적2．34，1．66배。2，3-BD 여2R，3R-BD 유도포복전고영항갈반병적과정중，항배혈산－곡광감태순배유지교고효솔삼여식물항병반응。
The compounds 2,3-butanediol (BD)and 2R,3R-BD can cause creeping bentgrass to show increased resistance against Rhizoctonia solani .To evaluate the mechanisms of this induced resistance,we determined the activities of antioxidant enzymes in the ascorbate-glutathione cycle and the redox states of various antioxi-dant compounds in leaves of creeping bentgrass that were injected with 250 μmol/L 2,3-BD and 100 μmol/L 2R,3R-BD before inoculation with R.solani.The 2,3-BD and 2R,3R-BD treatments significantly decreased the disease incidence in creeping bentgrass plants inoculated with R.solani and increased the activities of gluta-thione reductase (GR)and ascorbate peroxidase (APX).However,the increase in APX activity was smaller than that in the control (no BD).In the plants treated with 2,3-BD and 2R,3R-BD,the ascorbic acid (AsA) content decreased at an early stage and increased at a later stage after inoculation,while the content of de-hydroascorbic acid (DHA)peaked at 1 and 9 d after inoculation at levels significantly higher than those in the control.The AsA/DHA ratios of plants treated with 2,3-BD and 2R,3R-BD reached a maximum at 5 days af-ter inoculation (5.0 and 3.4 times the AsA/DHA ratio in the no-BD control at 5 d after inoculation,respective-ly).In plants treated with 2,3-BD and 2R,3R-BD,the glutathione (GSH)content significantly increased and the GSH/GSSG ratios peaked at 9 days after inoculation at 2.34 and 1.66 times that in the no-BD control,re-spectively.These results suggest that in creeping bentgrass,the resistance against R.solani induced by 2,3-BD and 2R,3R-BD involves the ascorbate-glutathione cycle,which maintains efficient metabolism and participates in the plant disease resistance response.
