CAJ | 학술논문

为了探明立枯丝核菌对苜蓿种苗的致病性，本研究采用菌饼上放置种子的方法，将分离自3个地点6个苜蓿品种上的6个菌株接种于14个紫花苜蓿品种的种子上，14 d 测定相对发芽率、根长、苗长和病情指数。结果表明，参试菌株的菌降低了种子的发芽，从金皇后分离到的菌株对苗长的抑制作用最强；致病性差异显著（P ＜0．05），其中从金皇后分离出菌株的致病性最强，从丰宝中分离出菌株的致病性最弱；从金皇后、8920MF 和爱菲尼特中分离出的立枯丝核菌对苜蓿种子与幼苗的致病力较强；参试的苜蓿品种对同一菌株的抗病性不同，佛拿尔、阿尔冈金和苜蓿王的抗病力最强，丰宝、丰叶721抵抗力最弱。
위료탐명립고사핵균대목숙충묘적치병성，본연구채용균병상방치충자적방법，장분리자3개지점6개목숙품충상적6개균주접충우14개자화목숙품충적충자상，14 d 측정상대발아솔、근장、묘장화병정지수。결과표명，삼시균주적균강저료충자적발아，종금황후분리도적균주대묘장적억제작용최강；치병성차이현저（P ＜0．05），기중종금황후분리출균주적치병성최강，종봉보중분리출균주적치병성최약；종금황후、8920MF 화애비니특중분리출적립고사핵균대목숙충자여유묘적치병력교강；삼시적목숙품충대동일균주적항병성불동，불나이、아이강금화목숙왕적항병력최강，봉보、봉협721저항력최약。
The aim of this study was to evaluate the pathogenicity of Rhizoctonia solani isolates to various alfal-fa (Medicago sativa )varieties.We used 14 varieties of alfalfa and six pathogen isolates that were isolated from roots of six different alfalfa varieties from three locations.We estimated the disease index,relative germination rate,relative root length,and relative seedling length at 14 days after inoculation with the various isolates. There were significant differences among the 14 alfalfa varieties inoculated with the six pathogen isolates (P <0.05).The isolate from ‘Goldenqueen’showed significantly stronger pathogenicity (P <0.05)than the other isolates.The isolate from ‘Powerplant’showed the weakest pathogenicity.All the fungi used in this study re-sulted in lower germination rates and shorter roots of alfalfa seedlings,and the isolate from ‘Goldenqueen’ showed the strongest effects.Based on the comprehensive evaluation of all traits,R.solani isolated from‘Goldenqueen’,‘8920MF’,and ‘Affinity’showed the strongest pathogenicity to alfalfa seedlings.Among the 14 alfalfa varieties,‘Vernal’,‘Algonguin’,and ‘Alfaking’were more resistant to R.solani,while ‘Power-plant’and ‘Amerileaf 721’were the most sensitive.