CAJ | 학술논문

为了探索微生物除臭剂对畜禽粪便环境污染的治理效果，本研究利用平板划线分离法从5份样品中分离出130株菌株，通过氨气和硫化氢选择性培养基定性初筛和硼酸吸收法、锌胺络盐吸收比色法定量复筛相结合的方法，分别筛选出了可高效抑制氨气、硫化氢释放的菌株5株和3株，其中命名为 BX3的菌株对氨气和硫化氢的去除率分别达到80．07％和76．92％；采用 L8（27）正交试验优化设计其高效除臭组合，结果表明，“AF2＋DZ1＋BX3＋DZ3＋BZ1＋EZ3＋AX4”组合氨气和硫化氢的释放量显著或极显著低于其他组合，为最佳组合；与空白对照相比，第5天时氨气和硫化氢的去除率达到82．14％和80．84％。对最佳组合除臭发酵工艺研究，在接种量分别为10％和15％，含水率40％和30％，麸皮添加量为10％时氨气和硫化氢的释放量最小。筛选的微生物在除臭剂制备及治理畜禽粪便对环境污染方面具有较大的应用潜力。
위료탐색미생물제취제대축금분편배경오염적치리효과，본연구이용평판화선분리법종5빈양품중분리출130주균주，통과안기화류화경선택성배양기정성초사화붕산흡수법、자알락염흡수비색법정량복사상결합적방법，분별사선출료가고효억제안기、류화경석방적균주5주화3주，기중명명위 BX3적균주대안기화류화경적거제솔분별체도80．07％화76．92％；채용 L8（27）정교시험우화설계기고효제취조합，결과표명，“AF2＋DZ1＋BX3＋DZ3＋BZ1＋EZ3＋AX4”조합안기화류화경적석방량현저혹겁현저저우기타조합，위최가조합；여공백대조상비，제5천시안기화류화경적거제솔체도82．14％화80．84％。대최가조합제취발효공예연구，재접충량분별위10％화15％，함수솔40％화30％，부피첨가량위10％시안기화류화경적석방량최소。사선적미생물재제취제제비급치리축금분편대배경오염방면구유교대적응용잠력。
By combining NH 3 and H2 S selective media for a first qualitative screening,and the boric acid ab-sorption and zinc amine complex salt absorption colorimetric methods for a second quantitative screening,5 strains which could efficiently reduce NH 3 release,and 3 strains reducing H2 S release were identified,of which a strain designated BX3 achieved emission reduction rates of NH 3 and H2 S,respectively,compared with control treatments,of 80.07%,76.92%.An L8 (2 7 )orthogonal optimum design was employed to identify combina-tions of different strains with high deodorizing efficiency,and AF2+DZ1+BX3 +DZ3 +BZ1 +EZ3 +AX4 was found to be the optimal strain combination.With this combination,the emission rates of NH 3 and H2 S were significantly lower than that of other combinations.Compared with a blank control group,the emission reduc-tions of NH 3 and H2 S,respectively,were 82.14%,80.84% on the fifth day of the experiment.The optimal microbial combination for deodorizing activity was studied under differing fermentation conditions and the quan-tities of NH 3 and H2 S released were smallest with additive combinations of 10% inoculation,30% moisture content and 10% bran addition,and 15% inoculation,40% moisture content and 10% bran addition,respec-tively.The microbial strains isolated in this study have potentially broad application prospects in the fields of odor prevention and control of environmental pollution caused by livestock and poultry manure.