CAJ | 학술논문

以野生早开堇菜为材料，研究了不同外植体类型（子叶节、叶片、叶柄）和不同激素配比对其不定芽诱导、愈伤组织诱导和分化的影响，成功建立了早开堇菜组织培养植株再生体系。结果表明，1）诱导子叶节和叶柄不定芽诱导的最适培养基为 MS＋2．0 mg／L 6-BA＋0．1 mg／L NAA，叶片不适合作为直接诱导不定芽的外植体。2）3种外植体均能诱导愈伤组织，子叶节愈伤组织诱导的时间最短，其次为叶柄，叶片最晚。子叶节和叶柄愈伤组织诱导的最适培养基为 MS＋1．0 mg／L 6-BA＋0．5 mg／L 2，4-D，诱导率分别为98．3％和96．7％；叶片愈伤组织诱导的最适培养基为 MS＋1．0 mg／L 6-BA＋0．5 mg／L 2，4-D＋0．05 mg／L NAA，诱导率可达88．3％。3）最适愈伤组织分化培养基为：MS＋1．5 mg／L 6-BA＋0．1 mg／L NAA，分化率为100％。4）不定芽增殖的最佳培养基为：MS＋1．5 mg／L 6-BA＋0．075 mg／L NAA，增殖率为4．68。5）再生苗移植到添加1．0 mg／L 6-BA 的1／2 MS 培养基上，生根率92．3％。6）组培苗移栽到珍珠岩∶河沙∶腐殖质（1∶2∶2）的混合基质时，100％成活，且植株长势好。
이야생조개근채위재료，연구료불동외식체류형（자협절、협편、협병）화불동격소배비대기불정아유도、유상조직유도화분화적영향，성공건립료조개근채조직배양식주재생체계。결과표명，1）유도자협절화협병불정아유도적최괄배양기위 MS＋2．0 mg／L 6-BA＋0．1 mg／L NAA，협편불괄합작위직접유도불정아적외식체。2）3충외식체균능유도유상조직，자협절유상조직유도적시간최단，기차위협병，협편최만。자협절화협병유상조직유도적최괄배양기위 MS＋1．0 mg／L 6-BA＋0．5 mg／L 2，4-D，유도솔분별위98．3％화96．7％；협편유상조직유도적최괄배양기위 MS＋1．0 mg／L 6-BA＋0．5 mg／L 2，4-D＋0．05 mg／L NAA，유도솔가체88．3％。3）최괄유상조직분화배양기위：MS＋1．5 mg／L 6-BA＋0．1 mg／L NAA，분화솔위100％。4）불정아증식적최가배양기위：MS＋1．5 mg／L 6-BA＋0．075 mg／L NAA，증식솔위4．68。5）재생묘이식도첨가1．0 mg／L 6-BA 적1／2 MS 배양기상，생근솔92．3％。6）조배묘이재도진주암∶하사∶부식질（1∶2∶2）적혼합기질시，100％성활，차식주장세호。
The effects of various hormone ratios on adventitious buds,callus inductions and differentiation were investigated using the cotyledon node,leaf blade and petiole of Viola prionantha as explants.Finally,a plant regeneration system via tissue culture was established.The results showed that the optimal medium for adven-titious bud induction from the cotyledon node and petiole was MS+2.0 mg/L 6-BA+0.1 mg/L NAA,but the leaf blade was not suitable as an explant.Three kinds of explants could induce callus formation.The optimal medium for callus induction from the cotyledon node and petiole was MS+1.0 mg/L 6-BA+0.5 mg/L 2,4-D with the highest callus induction rate being 98.3% and 96.7%,respectively.The optimal medium for callus induction from the leaf blade was MS+1.0 mg/L 6-BA+0.5 mg/L 2,4-D+0.05 mg/L NAA with the highest callus induction rate being 88.3%.The best medium for callus differentiation was MS+1.5 mg/L 6-BA+0.1 mg/L NAA with the highest callus differentiation rate being 100%.The optimal medium for the proliferation of adventitious buds was MS+1.5 mg/L 6-BA+0.075 mg/L NAA,the highest proliferation times being 4.68. The rooting rate of regenerated plants was up to 92.3% on half strength MS medium supplemented with 1.0 mg/L 6-BA.Rooted plantlets were grown on mixture with perlite:sand:humus (1:2:2);survival rate was up to 100% with good growth.