CAJ | 학술논문

在前期获得 DREB1A／Bar 双价转基因马铃薯的基础上，对转基因植株进行了耐旱性和除草剂抗性分析。耐旱性分析显示，在正常浇水条件下，对照和各转基因马铃薯株系生长状态良好且大致相同，各株系的丙二醛含量、相对电导率和 SOD 酶活性无显著差异（P ＞0．05）。经过控水10 d 后，非转基因对照植株叶片明显萎蔫卷曲，而转基因植株仍然保持良好的生长状态；转基因株系的丙二醛含量和相对电导率显著低于非转基因株系（P ＜0．05），而SOD 酶活性显著高于非转基因对照（P ＜0．05）。控水18 d 时，大部分对照植株死亡，死亡率为74．33％；转基因植株只有极少数植株死亡，DR2和 DR5的死亡率分别为20．43％和5．65％。用0．3％的市售草铵膦喷施各株系，10 d后，对照植株全部枯死，转基因株系的个别叶片干枯，绝大多数叶片及所有茎秆生长状态良好。以上分析表明， DREB1A 和 Bar 基因的导入，明显增强了转基因马铃薯对干旱和除草剂的抗性。
재전기획득 DREB1A／Bar 쌍개전기인마령서적기출상，대전기인식주진행료내한성화제초제항성분석。내한성분석현시，재정상요수조건하，대조화각전기인마령서주계생장상태량호차대치상동，각주계적병이철함량、상대전도솔화 SOD 매활성무현저차이（P ＞0．05）。경과공수10 d 후，비전기인대조식주협편명현위언권곡，이전기인식주잉연보지량호적생장상태；전기인주계적병이철함량화상대전도솔현저저우비전기인주계（P ＜0．05），이SOD 매활성현저고우비전기인대조（P ＜0．05）。공수18 d 시，대부분대조식주사망，사망솔위74．33％；전기인식주지유겁소수식주사망，DR2화 DR5적사망솔분별위20．43％화5．65％。용0．3％적시수초안련분시각주계，10 d후，대조식주전부고사，전기인주계적개별협편간고，절대다수협편급소유경간생장상태량호。이상분석표명， DREB1A 화 Bar 기인적도입，명현증강료전기인마령서대간한화제초제적항성。
In order to compare drought resistance of DREB1A/Bar transgenic potato plants,with a control cul-tivar (non-transgenic Longshu 10),5 -7 cm seedlings of DREB1A transgenic lines were grown in pots using vermiculite and regularly watered.Watering continued to the 15-16 leaf stage after which plants were not wa-tered for 18 days to impose for drought stress.During the drought stress,plant phenotypic features were re-corded using visual observation and digital camera images.After 10 days of drought stress,stress-related phys-iological and biochemical parameters were determined.During the normal watering phase both the non-trans-genic control and the transgenic potato lines grew well,the MDA content,relative electrical conductivity and superoxide dismutase (SOD)activity were not significantly different (P >0.05 ).After 10 days of drought stress the control plants began to wilt,but the transgenic lines remained in good condition.MDA content and relative electrical conductivity in the transgenic line was significantly lower than those of the non-transgenic plants (P <0.05),while the SOD activity was significantly higher than that of non-transgenic plants (P <0. 05).After for 18 days of drought stress 74.33% of the control plants were dead whereas fewer transgenic plants had died;the mortality of DR2 and DR5 was 20.43% and 5.65% respectively.The resistance of DREB 1A/Bar transgenic potatoes to glufosinate,a systemic non-selective herbicide,compared with non-trans-genic lines was determined by treating plants with a 0.3% glufosinate spray.Ten days after treatment all non-transgenic plants had died,while the transgenic plants were affected slightly.The study indicated that intro-duction of DREB1A and Bar genes significantly enhanced drought tolerance and herbicide resistance in trans-genic potato plants.